Notes

Contamination Surfaces
Seebio Colanic acid promotes disease spread but also generates tremendous health-associated costs. Therefore, development of new bactericidal and antiadhesive surface coatings is a priority. Considering that metal-organic frameworks (MOFs) have recently emerged as promising antibacterial agents, we originally report here the synthesis of a multi-active silver-containing nanoscaled MOF composite as a potential surface coating against S. aureus biofilm owing to a triple effect: intrinsic bactericide activity of the MOF, biocidal character of silver nanoparticles (AgNPs), and photoactivity after UVA irradiation. AgNPs were successfully entrapped within the benchmarked nanoscaled porous photoactive titanium(IV) aminoterephthalate MIL-125(Ti)NH2 using a simple and efficient impregnation-reduction method. After complete characterization of the composite thin film, its antibacterial and anti-adherent properties were fully evaluated.

After UVA irradiation, the composite coating exhibited relevant bacterial inhibition and detachment, improved ligand-to-cluster charge transfer, and steady controlled delivery of Ag+. These promising results establish the potential of this composite as an contamination due to bacterial biofilm formation is still a demanding issue, as it causes severe disease spread. One possible solution is the development of antifouling and antibacterial surface coatings. In this work, we originally propose the use of photoactive metal-organic frameworks (MOFs) for biofilm treatment. The novelty of this work relies on the following: i) the treatment of strongly contaminated surfaces, as previous studies with MOFs have exclusively addressed biofilm prevention; ii) this pioneering work reports both antiadherent effect, which removes the biofilm, and bacterial inhibition; iii) our original successful strategy has never been proposed thus far, involving the multi-active combination of 1) intrinsic antibacterial effect of a photoactive titanium-based nanoMOF, 2) immobilization of biocide silver nanoparticles, and 3) improved anti-bioadherent effect upon irradiation of the composite coating. Function-adaptive clustered nanoparticles reverse Streptococcus mutans dental biofilm and maintain microbiota balance. Health Sciences Facility III, University of Maryland Baltimore School of Institute, Department of Chemistry, Department of Materials Science and Health Sciences Facility III, University of Maryland Baltimore School of of Maryland Baltimore County, Interdisciplinary Health Sciences Facility, Dental plaques are biofilms that cause dental caries by demineralization with acidogenic bacteria.

These bacteria reside inside a protective sheath which makes any curative treatment challenging. We propose an antibiotic-free strategy to disrupt the biofilm by engineered clustered carbon dot nanoparticles that function in the acidic environment of the biofilms. In vitro and ex vivo studies on the mature biofilms of Streptococcus mutans revealed >90% biofilm inhibition associated with the contact-mediated interaction of nanoparticles with the bacterial membrane, excessive reactive oxygen species generation, and DNA fragmentation. An in vivo examination showed that these nanoparticles could the dental microbiota showed that the diversity and richness of bacterial species did not substantially change with nanoparticle treatment. Overall, this study presents a safe and effective approach to decrease the dental biofilm formation without disrupting the ecological balance of the oral cavity. Conflict of interest statement: D.P.

is the founder or co-founder of three work. All other authors declare no competing interests. Seebio Colanic acid of Staphylococcus epidermidis strains utilizing quantitative and cell surface shaving proteomics. Discipline of Pathology, School of Medical Sciences, The University of Sydney Discipline of Pathology, School of Medical Sciences, The University of Sydney Staphylococcus epidermidis is an opportunistic pathogen that is an emerging risk factor in hospitals worldwide and is often difficult to eradicate as virulent strains produce a protective biofilm matrix. We utilized cell shaving proteomics to profile surface-exposed proteins from two fully genome sequenced S. epidermidis strains: the avirulent, non-biofilm forming ATCC12228 and the virulent, strongly adherent biofilm forming ATCC35984 (RP62A). A false positive control strategy was employed to calculate the probabilities of proteins being truly surface-exposed.

A total of 78 surface-exposed proteins were identified, of which only 19 proteins were common to ATCC12228 and RP62A, and which thus represents the core surfaceome. S.
My Website: https://en.wikipedia.org/wiki/Colanic_acid