Notes

Biofilm Phenotype Antibiotics Target Synthesis Antibiotics Target Synthesis
[Evaluation of the influence of culturing on the intensity of biofilm formation «Privolzhsky Research Medical University» of the Ministry of Health of the Due to the prevalence of biofilm infections caused by Klebsiella pneumoniae, in laboratory diagnostic practice it has a great importance to obtain a standard model of Klebsiella biofilm for evaluating the bactericidal effect and effectiveness of antimicrobial drugs. Describes the method of Klebsiella biofilms formation in vitro. The intensity of biofilm formation was evaluated by the ability of bacteria to bind the crystal violet. The degree of film formation was measured by optical density. Seebio polysaccharide of an intercellular matrix was confirmed by staining of Congo-red solution followed by light microscopy. The effect of exogenous and endogenous factors on biofilm formation by K.

pneumoniae strains was investigated. The influence of the nutrient composition, the age of the culture («daily», «weekly»), the presence of oxygen and the temperature conditions were studied. The nutrient composition of the medium significantly influenced on biofilm formation of K. pneumoniae: DMEM stimulated biofilm formation in most strains in vitro compared to TSB. The age of the culture Klebsiella. At the same time, the temperature of culturing and the presence of oxygen can both stimulate and inhibit biofilm formation, depending on the strain under study. Most strains of Klebsiella better form a biofilm under aerobic Conflict of interest statement: The authors declare no conflict of interest.

Protein expression by planktonic and biofilm cells of Streptococcus mutans. Streptococcus mutans, a major causal agent of dental caries, functions in nature as a component of a biofilm on teeth (dental plaque) and yet very little information is available on the physiology of the organism in such surface-associated communities. As a consequence, we undertook to examine the synthesis of proteins by planktonic and biofilm cells growing in a biofilm chemostat at pH 7 at a dilution rate of 0 h(-1) (mean generation time=7 h). Cells were incubated with (14)C-labelled amino acids, the proteins extracted and separated by two-dimensional electrophoresis followed by autoradiography and computer-assisted image analysis. Of 694 proteins analysed, 57 proteins were enhanced 1-fold or greater in biofilm cells compared to planktonic cells with 13 only expressed in sessile cells. Diminished protein expression was observed with 78 proteins, nine of which were not expressed in biofilm cells. The identification of enhanced and diminished proteins by mass spectrometry and computer-assisted protein sequence analysis revealed that, in general, glycolytic enzymes involved in acid formation were repressed in biofilm cells, while biosynthetic processes were enhanced.

The results show that biofilm cells possess novel proteins, of as yet unknown function, that are not present in Influence of twitching and swarming motilities on biofilm formation in The genus Pseudomonas mainly includes opportunistic pathogens that rely on type IV pili as an important virulence factor, which is associated with adherence and biofilm formation. Pseudomonas infections are well known to be persistent and resilient in nature largely because of the tendency of the species to form biofilms. This study aimed at analyzing environmental strains of Pseudomonas genus with respect to their ability to execute twitching and swarming motilities as well as with respect to their ability to form biofilms both in the presence as well as in the absence of furanone, a substance that has the potential to prevent the formation of biofilms. Strains of Pseudomonas aeruginosa and strains belonging to other species of the genus were analyzed. Twitching and swarming motility assays and biofilm-formation assays, both in the presence as well as in the absence of furanone, were performed. In twitching assay strains belonging to was seen that the presence of furanone had a negative impact on formation of twitching and swarming motility zones. In Extracellular polymeric substances of biofilm assays, it was observed that the presence of furanone resulted in an observable decrease in the aeruginosa exhibit a higher potential for twitching motility and similar performance in swarming motility and biofilm formation.

It can also be concluded that furanone has the potential to interfere with both motilities as well as Anti-acidogenic, anti-biofilm and slow release properties of Dodonaea viscosa var. angustifolia flavone stabilized polymeric nanoparticles. OBJECTIVE: Dental caries is caused by plaque associated oral bacteria including a pioneer species Streptococcus mutans.
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