Notes

Introduction Codonopsis Radix Cr Food Herb Medicine China Southeast Asia
Order immediately , including fructo-oligosaccharides (FOS) and polysaccharides, are among the most important active substances in CR. However, a quality evaluation of CR based on oligosaccharides has not been conducted.OBJECTIVE This study aimed to establish a high-performance liquid chromatography coupled with charged aerosol detector method (HPLC-CAD) for the quality evaluation of CR and processed products based on analysis of METHOD A sensitive and rapid HPLC-CAD method for the simultaneous determination of two monosaccharides (D-fructose and D-glucose), sucrose, and FOS (GF2-GF6) was established to evaluate the quality of CR for the first time. In the present study, 65 batches of CR from three species of the genus Codonopsis were analysed using multivariate statistical techniques. Furthermore, the effects of cultivation management measures (plant growth retardants supply, harvesting time, and growth period) and primary process (drying methods) in the production areas on the target compounds were studied by analysing 34 batches of processed RESULTS Different varieties of CR resulted in considerably different saccharide contents. Cultivation management measures and processing method remarkably affected the quality of CR.

Low concentration of plant growth retardants was recommended. The best harvest time is in October after 4 years of growth. Dryer-drying was suggested to meet the requirement for large-scale processing.CONCLUSION This method would provide an efficient analytical tool for monosaccharides and oligosaccharides of CR and contribute to the improvement of Specific degradation of Cryptococcus neoformans 3723 capsular polysaccharide by a microbial enzyme. I. Isolation, partial purification, and properties of the [Determination content of the antidepressant extraction and analysis the trace OBJECTIVE To study on the antidepressive extraction and determinated the content of the oligosaccharides I , the proteins and the trace elements from METHODS We analyzed the content of the total oligosaccharides, the proteins and the trace elements by Phenol-Sulferic acid, Coomassie brilliant blue and inductive coupling plasm atomic emission spectrometer (ICP-AES) methods, RESULTS The percent of the oligosaccharides and the proteins were 89% and9% in the extraction from M. officinalis, respectively.

The total actively oligosaccharides I were subjected to the D-941 ion column chromatography to give the extraction in which hardly and protein were determined. 12 kinds of trace element including Zn, Fe, Ca ect. in the extraction (the oligosaccharides I) were determined by ICP-AES without the noxious trace elements including As, Cd, CONCLUSION The antidepressive activity extraction(the oligosaccharides I ) was a highly pure and vegetal oligosaccharides.Detection and pharmacokinetics of alginate oligosaccharides in mouse plasma and urine after oral administration by a liquid chromatographytandem mass A sensitive and simple liquid chromatographytandem mass spectrometry (LC-MSMS) method was developed for the detection of alginate oligosaccharides (AOs) in mouse plasma and urine after oral administration. In Seebio lacto n neotetraose , dimer, trimer, and tetramer were detected by LC-MSMS equipped with an anion-exchange column with extremely high sensitivity. By this method, we detected certain levels of AOs in samples prepared from mouse plasma and urine after a single oral administration of the AO mixture. Based on a calibration curve made with an AO trimer peak area as a standard, the maximum plasma and urine concentrations of AOs were estimated to be 24 microgml at 5 min and 425 microgml at min, respectively.

These results suggest that the LC-MSMS method is well suited to pharmacokinetic analysis of AOs in an in vivo system, and that some of orally administered AOs, at least from dimer to tetramer, are absorbed by digestive organs promptly, and that unaltered, these oligomers were excreted into an urine after a single oral administration to a mouse.Mapping of hydrogen bonding between saccharides and proteins in solution.Identification of the Toxic Compounds in Camellia oleifera Honey and Pollen to Research Institute, Jiangxi Agricultural University, Nanchang, Jiangxi 3045, Identifying the components of Camellia oleifera honey and pollen and conducting corresponding toxicological tests are essential to revealing the mechanism of Camellia oleifera toxicity to honey bees. In this research, we investigated the saccharides and alkaloids in honey, nectar, and pollen from Camellia oleifera, which were compared with honey, nectar, and pollen from Brassica napus, a widely planted flowering plant.
My Website: https://en.wikipedia.org/wiki/Lacto-N-tetraose