Notes

There was significant correlation between meat color and chemical parameters, including total lipid hydroperoxides, thiobarbituric acid reactive substances, and metMb
However, there was no significant correlation between pH value and meat discoloration. These results suggest that ERT in the hydrophilic extract of F. velutipes plays an important role as a color stabilizer of meats.Importance of the methyl group at C10 of squalene for hopene biosynthesis and Graduate School of Science and Technology, Niigata University, Ikarashi, Niigata (6E,10E,14E,18E)-2,6,10,19,23-pentamethyl-tetracosa-2,6,10,14,18,22-hexaene with Alicyclobacillus acidocaldarius hopene cyclase afforded four products having two types of carbocyclic skeletons, i.e., two hopane products and two products having an unprecedented carbocyclic skeleton of 6/5 + 5/5 +6 pentacyclic and 6/5 + 5/5 tetracyclic ring systems.

The former two hopane skeletons were formed from the bioconversion of C15-desmethylsqualene and the latter two skeletons from A rapid HPLC post-column reaction analysis for the quantification of ergothioneine in edible mushrooms and in animals fed a diet supplemented with extracts from the processing waste of cultivated mushrooms.and Technology, Konan 4, Minato-ku, Tokyo 108-8477, Japan.and Technology, Konan 4, Minato-ku, Tokyo 108-8477, Japan. Electronic address: For establishing an efficient and sensitive method for the quantitative determination of 2-thiol-l-histidine-betaine (ergothioneine, ERG) in edible mushrooms and the blood and muscles of animals, a technique using reversed-phase separation and post-column reaction between 2'-dipyridyl disulphide and ERG was developed. A corresponding derivative 2-thiopyridone, detected at 343 nm, was used for estimating ERG concentration. The flow rate, temperature, pH, and composition of the solution were optimised. what is squalane of quantification (11 ppm) and a simpler sample preparation made this technique more rapid compared to other methods using liquid chromatography-mass spectrometry.

The coefficient of variation (CV) values for the reproducibility and recovery of ERG were within the acceptable values of 6% and 97-100%, respectively. squalane cleanser of this methodology was compared with that of spectrophotometric and mass-spectrometric quantitative methods, and was assessed in the light of previous studies. The ERG contents in different mushrooms were 129-2345 mg/kg wet weight basis. Dietary supplementation with extracts from mushroom processing waste significantly improved ERG bioavailability in the blood of yellowtail fish and muscle tissue of cattle.Heterocyclic thiols as antioxidants: why ovothiol C is a better antioxidant than Spectral, acid-base, and redox properties of 4-mercaptoimidazoles were investigated by pulse radiolysis in aqueous solutions. Thiyl radicals of 1-methyl-5-ethyl-4-mercaptoimidazole (MEMI) have weak absorption band at 330 nm, epsilon = 300 +/- 60 M-1 cm-1. Because the ionic strength variation from 01 to 0 M in the pH range from 3 to 14 does not influence the rate constant of the radical decay, it is concluded that the MEMI thiyl radical is neutral.

At pH 7, the reduction potential of the MEMI radical, E7 = 05 V, is lower than E7 = 08 V of the Trolox C radical, which means that MEMI may restitute vitamin E under physiological conditions (assuming similar reduction potentials of Trolox C and vitamin E radicals). Because pKa = 10 of the SH group in MEMI is lower than pKa = 11 of the OH group of Trolox C, the redox equilibrium with Trolox C is reversed at pH 13, and E13(MEMI-radical) = 09 +/- 04 V is determined against E13(Trolox C--radical) = 09 V. In contrast to extraordinary electron donating properties, MEMI is only a moderately good H-atom donor. k(.CH3 + MEMI) structure of MEMI. The ability of MEMI to act as antioxidant in biological systems is further demonstrated by its ability to efficiently scavenge superoxide and linoleate peroxyl radicals.(ABSTRACT TRUNCATED AT 250 WORDS)Squalene feeding in experimental atherosclerosis.

KRITCHEVSKY D, MOYER AW, TESAR WC, LOGAN JB, BROWN RA, RICHMOND G.Improvement of joint range of motion (ROM) and reduction of chronic pain after consumption of an ergothioneine-containing nutritional supplement.OBJECTIVE: To evaluate anti-inflammatory properties of a nutraceutical blend containing L-ergothioneine in concert with other anti-inflammatory and analgesic ingredients, combined with nutritional cartilage support.METHODOLOGY: Twelve human subjects were tested over a 6-week period of product consumption followed by a 6-week wash-out period, conducted at NIS Labs during late fall/early winter 2010. Range of motion (ROM) assessment of joint motility was performed using JTECH dual digital inclinometry and included flexion, extension, and rotation through the vertical weight-bearing column (neck, thorax, lumbar, hip, knees) and shoulders. Pain evaluation included questionnaires and Visual Analogues Scales regarding primary and secondary pain RESULTS: ROM improvements were seen after 1 week, and further improved at 6 weeks (primary pain area P<0, secondary pain area P<03). Pain in primary and secondary areas at use was significantly reduced already at 1 week, compared to baseline (P<05).
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