Notes

ERAP1 binds peptide C-termini of numerous series and/or programs with a common reputation mechanism.
The chemical analysis of archaeological ceramic artifacts can help archaeologists to characterize the technology used in the manufacturing process, and to elucidate aspects related to the way of life of past peoples. In this context, six ceramic fragments found at Tapera Beach in Florianópolis were analyzed. FTIR spectroscopy was used to identify carbonates and oxalic acid originating from the biodegradation process. In the pictorial regions of the ceramics, iron oxides, manganese and chromium were present, along with calcium carbonate, which were used as pigments. In addition, the absence of characteristic bands for kaolinite suggests low firing temperatures (up to 900 °C). EDS confirmed the elements of the molecules identified by FTIR and showed the presence of Al and Si in the primary structure and Fe in the secondary structure of the ceramics. In addition, there were traces of rare earth elements in samples A3 and A5, which may be related to the geochemistry at the site from which the raw material was excavated. The GC-MS results showed the presence of lipids and the profile of the fatty acids detected suggested that the source of the oils adsorbed in the ceramic structure is vegetable.The pandemic of coronavirus disease 2019 (COVID-19) showed a significant impact on routine daily services in departments of otorhinolaryngology head and neck surgery. The city of Wuhan, as the first reported epicenter in the world, resumed medical service since April 8, 2020. As the biggest ENT services provider in Wuhan, we share out institution's triage and screening system in the resuming period.
Current therapies for neuropathic pain are generally symptomatic and possess several side effects, limiting their prolonged usage.

Thus, it is urgent to develop novel and safe candidates for the management of this chronical condition. For this purpose, we investigated the analgesic effect of a standardized extract from Zingiber officinale Roscoe rhizomes (ZOE) obtained by CO
supercritical extraction, in a mice model of peripheral neuropathy. We also explored the mechanism of action of ZOE and its main constituents using an in vitro model of neuroinflammation.

Peripheral mono-neuropathy was induced in mice, by spared nerve injury (SNI). The analgesic effect of ZOE after oral administration was assessed by measuring mechanical and thermal allodynia in SNI mice. The mechanism of action of ZOE and its main constituents were investigated using spinal cords samples and in an in vitro model of neuroinflammation by ELISA, western blotting and immunofluorescence techniques.

Oral administration of ZOE 200 mg kg
ameliorated mechanical and thermal allodynia in SNI mice, with a rapid and a long-lasting effect. ZOE did not alter locomotor activity. In BV2 cells and spinal cord samples, ZOE, 6-gingerol and 6-shogaol reduced pERK levels, whereas ZOE and terpene fraction reduced HDAC1 protein levels, inhibited NF-κB signalling activation and decreased IL-1β, TNF-α and IL-6 release. ZOE and each tested constituent had a positive effect on inflammation-impaired SH-SY5Y cell viability.

The oral administration of ZOE attenuated SNI-induced neuropathic pain symptoms by reducing spinal neuroinflammation, suggesting ZOE as a novel and interesting candidate for the management of neuropathic pain.
The oral administration of ZOE attenuated SNI-induced neuropathic pain symptoms by reducing spinal neuroinflammation, suggesting ZOE as a novel and interesting candidate for the management of neuropathic pain.New, sensitive and rapid chiral HPLC method for enantioseparation and determination of widely used hypnotic drug, zopiclone, was developed. Enantioseparation was achieved on the immobilized amylose based Lux i-Amylose 1 column in polar organic mode in less than 7 min. The effects of the mobile phase composition, type and content of major components as well as acid/base ratio and column temperature on the retention and enantioseparation were investigated. The mobile phase consisted of acetonitril and methanol with small addition of triethylamine and acetic acid with a flow rate of 1 mL min-1. Calibration curves of both zopiclone enantiomers were linear over the concentration range of 5-125 μg mL-1. The limits of detection and quantification for (R)-zopiclone were 5 and 15 ng mL-1 and for (S)-zopiclone were 7 and 21 ng mL-1, respectively. It was demonstrated that the proposed method was selective, precise and robust. Finally, the validated method was applied to the determination of zopiclone enantiomers in the commercial tablets.Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal carcinomas due to an inefficient early diagnosis and a disastrous prognosis. High resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) detection of pancreatic tissues would facilitate the understanding of metabolomic characteristics of PDAC and further its clinical diagnosis by in vivo magnetic resonance spectroscopy (MRS). Pancreatic tissues from PDAC patients and Sprague-Dawley (SD) rats model and corresponding controls were detected and comparatively analyzed with HR-MAS NMR-based metabolomic strategy in order to get the underlying biomechanism and diagnostic information of PDAC. According to the univariate and multivariate statistical analysis, eight shared characteristic metabolites by PDAC patients and rats, including glycerophosphocholine (GPC), lactate, myo-inositol, methanol, taurine, methylene of lipid (L-CH2), β-glucose and phosphocholine (PC), were identified as potential biomarkers of PDAC. Especially, GPC, PC and myo-inositol demonstrated high levels in pancreatic tissue and kept consistent metabolic changes both in PDAC patients and rat models. The occurrence of PDAC mainly involved the aberrations in glycerophospholipid metabolism, galactose metabolism and taurine and hypotaurine metabolism. find more As an in vitro alternative technique of in vivo MRS, HR-MAS NMR provided good resolution and sensitivity, thus underpinning a potential translation to the in vivo MRS setting for noninvasive detection and monitoring of clinical PDAC. The metabolic differences caused in the different species not only enhance the understanding of metabolic reprogramming of PDAC but also promote the intercommunication of PDAC metabolomes between the different species.Hypochlorous acid (HOCl) is involved in numerous cellular processes, such as pathogen response, immune regulation, and anti-inflammation. Consequently, the development of HOCl detection at the cellular level has been an important issue in investigating the dynamic distributions of HOCl. Herein, a fluorescent probe, Lyso-NA, containing a HOCl-reactive aminophenol group and a lysosomal-targeting morpholine group, has been effectively designed for detecting lysosomal HOCl. The reaction of Lyso-NA with HOCl induces the oxidation of aminophenol and accompanied by a 136-fold fluorescence enhancement. The detection limit is found at 13 nM. The fluorescence enhancement is accomplished through the suppression of twisted intramolecular charge transfer (TICT). With morpholine, the probe Lyso-NA shows the great lysosomal targetable ability for imaging endogenous lysosomal HOCl in living cells and tissues by two-photon microscopy, providing an opportunity to monitor HOCl in the lysosomes for understanding its biological functions.Accumulating evidence has been suggesting that combining two or more anticancer drugs can provide additive or synergistic effects, improving therapeutic efficacy and delaying resistance. Nowadays, advances in nanotechnology-based delivery systems have enabled the association of different drugs into a single carrier and provided therapeutic gains to the proposed regimen. However, a new strategy also requires innovative analytical approaches that assess loading capacity, biological performance, and also comprehend the mechanisms of action. Alpha-cyano-4-hydroxycinnamic acid (CHC) and the monoclonal antibody (mAb) cetuximab (CTX) are explored worldwide for their therapeutic benefits against multiple cancer cells. The present work aims to develop and validate a new method for simultaneous quantification of CHC and CTX in nanoparticulate systems by using reverse phase high-performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detection for CHC, and fluorescence detection for CTX. This method was designfy CHC and CTX in future studies applied to anticancer therapy.Greening analytical methods has become of great interest in the field of pharmaceutical analysis to protect both the operators' health and the environment. In this work, an innovative methodology combining Quality-by-Design (QbD) and Green Chemistry principles was followed to develop a single, green and robust RP-HPLC method for the quantitative analysis of impurities of both artesunate and amodiaquine drugs. Ethanol was selected as the best ecofriendly alternative solvent in substitution to the commonly used organic solvents such as acetonitrile and methanol. To achieve method objectives, resolutions between the 10 peaks were chosen as critical method attributes (CMAs) to be optimized through QbD approach. Based on a quality risk assessment, pH, temperature, and gradient slope were then selected as critical method parameters (CMPs) and a three level full factorial design was used to model the CMAs as function of the CMPs. Response surface methodology associated to Monte Carlo simulations allowed to determine the method operable domain region (MODR), i.e., the multidimensional combination of CMPs where CMAs simultaneously satisfied specifications (Rs ≥ 1.5) with a probability at least equal to 95 %. Inside the MODR, the working point was chosen based on green criteria, involving a mobile phase composed of ethanol and 10 mM acetic acid only as pH modifier. The method was successfully validated for all impurities using accuracy profile methodology, which was fully compliant with the ICH Q2(R1) requirements. Finally, the method was applied to the analysis of amodiaquine and artesunate impurities in raw materials and formulations.Harmful illicit drug use, such as opioid use disorder (OUD), causes multiple diseases that result in physiological, pathological, and structural changes in serum biochemical parameters based on the period of use. Fourier-transform infrared (FTIR) spectrometry is a noninvasive optical technique that can provide accurate evidence about the biochemical compounds of analytical samples. This technique is based on the detection of functional groups and the spectral analysis of the region of the selected bands, which provides a reliable and accurate tool for evaluating changes in the biochemical parameters of OUD patients. In the present study, the Attenuated Total Reflection (ATR)-FTIR technique and clinical laboratory biochemical results were used to investigate the phospholipid-protein balance in the blood serum of participants with OUD by comparing their data to that of healthy controls. To compare the biochemical laboratory results with serum vibrational spectroscopy, we used infrared (IR) spectroscopy to distints were explored. The results successfully specified the distinctions between OUD and the healthy controls (HCs). We compared the results with biochemical markers, such as albumin (Alb), Tg, and total cholesterol (Tc) levels of the patients, as well as the data of the healthy subjects obtained from the hospital. Additionally, we found that the Tg, Tc, and Alb levels decreased as the duration of heroin use increased based on the biochemical markers of the OUD patients. The laboratory biochemical reports and the vibrational spectroscopic analysis were correlated. The confidence of specificity, sensitivity, and accuracy was 100%, 92.85%, and 97.06% in the second derivative, respectively. Thus, we demonstrated that IR spectroscopy, multivariate data analysis, and clinical reports are consistent and correlated. Furthermore, FTIR is a simple and readily available diagnostic test that can successfully differentiate the serum samples of OUD patients from those of healthy subjects.
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