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Hypoxic preconditioning revitalizes mesenchymal originate cells and also improves neuroprotection pursuing intracerebral lose blood through the miR-326-mediated autophagy.
Chronically treating spontaneously hypertensive rats with increasing doses of crude hydroalcoholic E. grandiflorus extract resulted in significant dose-dependent reductions in systolic blood pressure and an anti-inflammatory effect on the brain microcirculation of DM-spontaneously hypertensive rat animals. Using laser speckle contrast imaging, we observed that intravenous administration of the major isolated clerodane diterpene metabolite (1 - 10 mg/kg) increased microvascular blood flow by 25% in spontaneously hypertensive rat skeletal muscle. The results of this study show that E. grandiflorus extracts can be useful in the prevention and reduction of microcirculatory damage in arterial hypertension and other diseases that involve microvascular dysfunction. Georg Thieme Verlag KG Stuttgart · New York.Zingiber montanum rhizomes are traditionally used for the treatment of numerous human ailments. The present study was carried out to investigate the inhibitory activity of the crude extract, chromatographic fractions, and purified compounds from Z. montanum rhizomes on the migration of MDA-MB-231 cells. The effect of the extract on cell migration was investigated by a scratch assay, which showed significant inhibition in a concentration-dependent manner. Vacuum liquid chromatography on silica gel afforded four fractions (Frs. 1 - 4), which were tested on cell migration in the scratch assay. Frs. 1 and 2 showed the most significant inhibition of MDA-MB-231 cell migration. The effect of the most potent fraction (Fr. 2) was further confirmed in a transwell migration assay. The study of Frs. 1 and 2 by gelatin zymography showed significant inhibition of MMP-9 enzyme activity. Chromatographic separation of Frs. 1 and 2 afforded buddledone A (1 ), zerumbone (2 ), (2E,9E)-6-methoxy-2,9-humuradien-8-one (3 ), zerumbone epoxide (4 ), stigmasterol (5 ), and daucosterol (6 ). In a cell viability assay, compounds 1  - 4 inhibited the viability of MDA-MB-231 cells in a concentration-dependent manner. The study of buddledone A (1 ) and zerumbone epoxide (4 ) on cell migration revealed that 4 significantly inhibited the migration of MDA-MB-231 cells in both scratch and transwell migration assays. The results of the present study may lead to further molecular studies behind the inhibitory activity of zerumbone epoxide (4 ) on cell migration and support the traditional use of Z. montanum rhizomes for the treatment of cancer. Georg Thieme Verlag KG Stuttgart · New York.The aim of the study was to highlight the aspect of oral and cutaneous changes in anorexia nervosa (AN) as a purging type. On the basis of three clinical cases, a description has been made of the beginning and the possible course of the disease, teeth, oral mucosa, and skin changes resulting from the illness. The research method was based on the analysis of the available literature on AN supplemented by clinical experience of presented cases. On the basis of the interdisciplinary analysis, it was concluded that the appropriate diagnosis and treatment of AN are possible only with the collaboration of many specialists-psychiatrist or pediatric psychiatrist, general practitioner, dermatologist, dentist, and if necessary other medical specialists. In this context, psychotherapy is an integral aspect of treatment. Therefore, early intervention is essential to obtain good results of treatment.OBJECTIVES  This in vitro study investigated the remineralization potential of 1450 ppm, fluoride-containing toothpastes containing different active remineralization agents after cariogenic challenge with pH cycling. The enamel surface roughness after brushing and the chemical and physical characteristics of the toothpastes tested were also analyzed. MATERIALS AND METHODS  Fifty-six bovine enamel blocks were obtained (4 × 4 × 6 mm) and divided into three thirds intact (untreated), demineralized (artificial caries lesion), and treated (caries lesion, pH cycling, and brushing with dentifrices). Seven commercially available fluoride toothpastes (1450 ppm F) three with anti-erosion claims (Candida Professional [CPP], Colgate Total 12 Daily Repair [CDR], Regenerate Enamel Science [RES]); three with desensitizing claims (Bianco Pro Clinical [BPP], Elmex Sensitive [ESS], and Regenerador Diário DentalClean [RDC]); and one standard regular-fluoride toothpaste Colgate Total 12 (CTT) were selected. During pH cycling (delationship between surface remineralization and subsurface remineralization. Although toothpastes CPP and RDC present the lowest %SHR means, both products effectively remineralize within the subsurface carious lesion. Regression analysis demonstrated no strong correlations of the enamel surface roughness with the chemical and physical parameters. CONCLUSIONS  Most but not all the fluoride toothpastes were able to remineralize the enamel surface. No specific chemical or physical parameter alone correlated with the surface roughness.OBJECTIVES  The objective was to assess modes of failures under static load (SL) among titanium (Ti) and one- and two-piece zirconia abutment (ZA) in vitro. MATERIALS AND METHODS  The Ti abutments were digitally scanned for the fabrication of the one- and two-piece zirconia abutment specimens. This was done to standardize the design of the one-piece abutment and make it the blueprint of the Ti abutment. Twenty-one implant abutments and 21 implant replicas were categorized into three groups as follows group 1 (Titanium group), group 2 (one-piece ZA group), and group 3 (two-piece ZA group). A 250K-cycle, linear fatigue-load, reaching 10 to 210 Newton (N), was put on all specimens using an all-electric dynamic test instrument and the specimens were loaded until fracture. STATISTICAL ANALYSIS  Assessment of mode of fracture among the groups was done visually. Significance was based below 0.05. RESULTS  Screw fracture (n = 7) and abutment bending at the apical part (n = 7) occurred in the Ti group. In the one-piece zirconia group, screw and abutment fractures occurred in seven and seven cases, respectively. In the two-piece zirconia group, screw fracture (n = 7) above the Ti zirconia junction (transgingival segment) and abutment fracture (n = 7) were determined as the failure modes. CONCLUSION  In conclusion, all abutments underwent failures under SL in vitro; and the mode of failure modes varied among the different abutment designs used.OBJECTIVE  The aim of study was to evaluate in vitro the surface hardness, sorption, solubility, and color stability of three light-cured resin cements, namely RelyX Veneer (RLX), Variolink Veneer (VLK), and All Cem Veneer (ACV). MATERIALS AND METHODS  Cylindrical samples (15 × 1 mm) were made for each group using a metallic mold (n = 10). Vickers microhardness test was performed, and average hardness was calculated from three indentations (300 gf/15s) per sample. The sorption and solubility of the materials were evaluated according to ISO 40492009 based on three samples weighing initial (m 1), after immersion in distilled water for 7 days (m 2), and final (after removal of all moisture [m 3]). The color change was observed using a digital spectrophotometer, at three different time points, baseline, 1 day, and 1 week of immersion in coffee and distilled water (control). STATISTICAL ANALYSIS  Shapiro-Wilk test was used to analyze the normality of the data, and groups were compared using Kruskal-Wallis and Mann-Whitney tests. A significance level of 5% was used. RESULTS  RLX showed the highest microhardness mean values (36.96 VHN), but higher sorption (23.2 µg/mm3) and solubility (2.40 µg/mm3), with statistically significant differences with the other groups. For color stability, higher ∆E was observed for the samples immersed in coffee (p = 0.009). The VLK resin cement presented statistically significant differences from the other groups, with higher color changes in coffee at 1 day (15.14) and after 1 week (23.65). CONCLUSION  RLX resin cement showed better hardness results. All materials tested performed satisfactorily for sorption and solubility according to ISO 40492009. All materials showed high-staining values after 1 week of immersion in coffee.OBJECTIVE  Dental pulp stem cells (DPSCs) can be used as a component in the formation of regenerative dentine during direct pulp capping therapy. Concanavalin A (ConA) is a type of lectin with a molecular weight of 26 kDa derived from the Canavalia ensiformis plant. Lectins possess strong proliferation and differentiation abilities in various animal cells including lymphocytes, osteoblasts, and chondrocytes. The aim of study was to determine the effect of ConA on the proliferation and osteogenic differentiation of DPSCs in vitro. MATERIALS AND METHODS  In this in vitro study, DPSCs were isolated from third molars before ConA induction was performed at concentrations of 5 and 10 μg/mL. The proliferation assay was determined by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Osteogenic differentiation was determined by means of mineralization. STATISTICAL ANALYSIS  Data were analyzed using analysis of variance and a Student's t-test. Lonafarnib datasheet The p-value was set at 0.05. RESULTS  The addition of 5 and 10 µg/mL of ConA to DPSCs can significantly increase the proliferation and osteogenic differentiation of DPSCs (p ≤0.05). CONCLUSION  ConA can increase the proliferation and osteogenic differentiation of DPSCs.OBJECTIVES  The aim of this research was to develop a sensor of approximation by reflectance for guided surgery with dental implants without flap detachment, and verify the effectiveness of this system. MATERIALS AND METHODS  Ten models of total edentulous arches were divided into two groups. Two implants of 3.5 × 11.5 mm (NeoDent) were inserted in each model; in Group 1 (G1), a stereolithographic guide NeoGuide system was used. In Group 2 (G2), the experimental approximation sensor was used for the insertion of the implants. The evaluation of the results was performed by overlapping the virtual planning images with the tomographies of the models of the implants inserted. RESULTS  There were no statistically significant differences between the guide and the sensor groups. The averages and standard deviations observed at the angulation of the guide was 4.15 (2.65 degrees) and 5.48 (2.85 degrees) at the sensor. The linear deviations at the cervical level were 0.002 (1.37) and 0.11 (1.47) mm and at the apical level 0.19 (1.28) and 0.21 (1.42) mm, respectively. CONCLUSIONS  The use of a guide is important for the stabilization of the drills; the greatest challenge is to control the apical position of the implants, especially in highly reabsorbed edges. The experimental sensor can become an auxiliary tool to the stereolithographic guides; however, several difficulties must still be overcome to recommend the use of a sensor.OBJECTIVES  This study aims to compare the stress distribution and displacement that resulted from the use of a Gerber space regainer and sagittal distalizer using three-dimensional finite element analysis. MATERIALS AND METHODS  Three-dimensional simulated models of the appliances were developed using a software. The forces applied by the two appliances were 3N (tipping) and 15N (bodily), respectively. Displacement and von Mises stress on the compact and cancellous bone, periodontal ligament (PDL), crowns of the mandibular first, second permanent molars, and deciduous canines were calculated. Stress distribution and displacement values were measured via linear static analysis. RESULTS  Gerber space regainer showed greater displacement than that produced by the sagittal distalizer at the first permanent molar. However, such displacement was less at the other tested points when compared with that delivered by sagittal distalizer. The stresses created by Gerber appliance were higher in the crown and PDL of the deciduous canine than the crown of the first permanent molar crown.
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