Notes

Aftereffect of Temperature Treatment Process upon Microstructure as well as Fatigue Conduct of the Nickel-Base Superalloy.
Synovial sarcoma is typical aggressive malignant without satisfactory treatment outcome in adult series. Cyclin-dependent kinases (CDKs) in transcription have been considered promising molecular targets in cancer. Among these, CDK7 has been shown to play important roles in the pathogenesis of malignancies. However, the modulation mechanism of CDK7-regulated transcription in synovial sarcoma is unknown. In the present study, we aim to determine the expression and function of CDK7 in the transcription cycle of RNA polymerase II (RNAP II), and evaluate its prognostic and therapeutic significance in synovial sarcoma. Results showed that overexpression of CDK7 correlates with higher clinical stage and grade, and worse outcomes in clinic. High CDK7 expression was confirmed in all tested human synovial sarcoma cell lines and CDK7 was largely localized to the cell nucleus. Downregulation through siRNA or inhibition with the CDK7-targeting agent BS-181 exhibited dose-dependent cytotoxicity and prevented cell colony formation. Western blots demonstrated that inhibition of CDK7 paused transcription by a reduction of RNAP II phosphorylation. Blocking CDK7-dependent transcriptional addiction was accompanied by promotion of apoptosis. Furthermore, the CDK7-specific inhibitor reduced 3D spheroid formation and migration of synovial sarcoma. Collectively, our findings highlight the role of CDK7-dependent transcriptional addiction in human synovial sarcoma. CDK7-specific cytotoxic agents are therefore promising novel treatment options for synovial sarcoma.Agency reflects an orientation in which the individual prioritizes the self. Agency has been associated with positive consequences, such as directing people to accomplish tasks and attain goals. Yet, agency is also associated with negative consequences, such as fueling materialistic and narcissistic behavior. This paper reviews recent research that conceptualizes agency as a hierarchical framework. Specifically, a general orientation toward agency can be rooted in two distinct and separable motives self-efficacy and self-enhancement. Specifically, these motives can differentially guide how agency affects consumption and psychological well-being. As such, recognizing the distinct motives underlying agency allows for greater precision in predicting and understanding both consumer behavior in particular and human behavior in general.The author comprehensively reviews compulsive shopping (CS), a disorder characterized by excessive shopping and/or spending that leads to subjective distress and impaired functioning. CS has been linked to substance use disorders, mood, and anxiety disorders, and to the obsessive-compulsive spectrum. More recently, CS has been considered a behavioral addiction. Most CS experts consider CS an independent disorder. CS has an estimated prevalence of 5% in the United States general population with an onset in the late teens/early 20s. Psychiatric comorbidity is common, including mood, anxiety, substance use, and personality disorders. Little is known about its neurobiology and genetics. There are no standard treatments, but cognitive-behavioral group therapy appears promising. Future research should focus on validating the disorder and developing effective treatments.People seek meaning in the marketplace, but can meaning be bought? We review emerging evidence and suggest that the typical association between meaning and well-being is weakened in consumption contexts. We outline two lay beliefs that help explain this gap the belief that purchases are extrinsic pursuits whereas meaning should come from intrinsic pursuits, and the belief that purchases are impure sources of meaning because companies profit at the expense of people. This conceptual model suggests three paths to enhance meaning and well-being through consumption reframe purchases as intrinsically rewarding, change (erroneous) lay theories that profit necessarily comes at the expense of the social good, or highlight the future, enduring benefits of consumption.People with a strong materialistic value orientation (MVO) believe that the acquisition of more money and expensive material possessions will improve their wellbeing and social standing. Paradoxically, striving for evermore money and material goods as a means of improving wellbeing often undermines quality of life. This paper documents how MVO has been linked to poorer wellbeing across different facets of wellbeing (personal, social, and environmental) and that these negative associations have been recorded across the lifespan. However, it also shows that the link is complex in that it can be moderated by certain personal and cultural factors and is bidirectional in its nature. By demonstrating a predominantly negative effect of MVO on wellbeing, the evidence highlights a need for interventions to reduce MVO and alter how people relate to material possessions.One core fundamental need that people may try to address through consumption is that of affiliation, or the need to belong. First, giving gifts may serve that goal, but it may also potentially hurt the formation of affiliative bonds if it creates a sense of indebtedness. Second, experiential consumption serves the need to belong if it is enjoyed socially. Third, symbolic consumption may help craft a prosocial image, but may also hinder it if one engages in conspicuous consumption to demonstrate one's wealth. Finally, consumption of media that allows the formation of parasocial relations with influencers and celebrities, and presence and activity in online communities can act as a surrogate for offline satisfaction of the need to belong.Few cases have been reported of the diagnosis and treatment of glioblastoma (GB) during pregnancy. Subsequently, surgical, medical, and obstetrical management of complicated primary central nervous system malignancy in antepartum and postpartum patients remains under-investigated. The authors report the case of a 24-year-old female patient who developed generalized tonic-clonic seizures and focal neurologic deficits. MRI imaging (3T Skyra, Siemens, Erlangen, Germany) revealed an intracranial mass suspicious for malignant tumor and surgical resection under awake sedation was scheduled. The patient was incidentally found to be in her first trimester of pregnancy. Using neuronavigation, neurophysiologic monitoring, and conscious sedation the tumor was debulked successfully and histopathologic analysis confirmed giant cell glioblastoma, WHO Grade IV, 1p/19q intact, IDH wild-type, with NF1 p.Y2285fs and RB1 p.S318fs somatic mutations. Post-surgical oncologic management continued with fractioned radiotherapy and use of the Optune® device. The patient underwent uncomplicated cesarean section at 34-weeks gestation, the child remains healthy and the patient remains disease-disease free at 1-year. Thus, this case presents an approach to management of complicated GBM during first trimester pregnancy.Cobalt-based nanoparticles (CBNPs) have recently received great attention in biomedical studies; however, the possible biotoxicity of these nanoparticles (NPs) has remained a foremost concern that should be addressed. As surface functionalization is one of the helpful proposed solutions, we aimed to apply Lipoamino acids (LAAs) as a coating agent to improve biocompatibility. To this purpose, cobalt oxide, cobalt ferrite, and iron oxide nanoparticles (IONs) were synthesized with and without 2-amino-hexadecanoic acid coating to assess the impacts of LAA coating on characteristics and biocompatibility of CBNPs in human cells and compare with IONs, a widely used magnetic NPs in biomedicine. selleck chemicals llc Antibacterial activities of NPs were evaluated against four Gram-negative and Gram-positive bacteria species to assess their biointerface interaction with prokaryotic cells. In addition, the antibacterial activities of synthesized NPs were compared to silver NPs, one of the widely used antimicrobial NPs and standard antibiotics (ampicillin). The structural characteristics properties of NPs were analyzed using TEM, FE-SEM, EDS, FTIR, XRD, and VSM. These NPs exhibited sphere-like to polygon-like morphology with desirable mean size. CBNPs displayed dose-dependent cytotoxicity and antimicrobial activities against human cell lines and all tested microbial species, as well as more cytotoxicity and bacterial inhibition compared to IONs. Besides, the results revealed that LAA coating could significantly improve the biocompatibility and antibacterial activity of NPs while impacting magnetic properties. To sum up, it seems that surface functionalization could provide more potent tools for bioapplications with improving biocompatibility and bacterial inhibition of CBNPs, though; further studies are needed in this regard.The aim of this study is to develop a dual-functional ingredient with antioxidant activity and emulsification. The emulsion stability of ovalbumin (OVA) was improved by procyanidins (PC). The interactions between OVA and PC were investigated using multi-spectroscopy and molecular docking. Furthermore, the effect of the addition of the OVA-PC mixture on emulsion stability was evaluated as well. The fluorescence results showed that the quenching mechanism of PC to OVA's endogenous fluorescence was static quenching, and the binding ratio of OVA and PC was 11. Circular dichroism (CD) and Fourier Transform Infrared Spectrometer (FT-IR) showed that the addition of PC promoted the unfolding of OVA, and transformed the secondary structure of OVA from α-helix to β-sheet. The main driving force of OVA and PC was hydrogen bonding, according to molecular docking analysis. Among all the samples, the stability of the emulsion of OVA-PC at a ratio of 130 exhibited extremely high stability and the smallest particle size. In comparison with individual OVA emulsions, the OVA-PC emulsions had excellent physical stabilities. Meanwhile, the oxidation degree of protein and oil for the OVA-PC emulsions was lower than that of the native OVA emulsion after 8-day storage. Our work provides important insights for understanding the interaction between OVA and expanding the application of OVA-PC.
Perilipin 5 (Plin5) acts as a pivotal mediator of oxidative stress and inflammation and is associated with the progression of relevant diseases. Cerebral ischemic stroke is a severe pathological condition that involves excess oxidative stress and inflammation. However, whether Plin5 plays a role in the progression of cerebral ischemic stroke remains unaddressed. This work focused on the investigation of Plin5 in oxygen-glucose deprivation/reoxygenation (OGD/R)-injured neurons, an in vitro model for studying cerebral ischemic stroke.

The primary neuronal cells were isolated from the hippocampus of newborn mice. Neurons were subjected to OGD/R treatment to establish an in vitro model for studying cerebral ischemic stroke. Neurons were infected with recombinant adenovirus expressing Plin5 to upregulate Plin5 expression. The mRNA levels were measured by real-time quantitative PCR (RT-qPCR). Protein levels were determined by immunoblotting. Cell viability was assessed via cell counting kit-8 (CCK-8) assay. Celf2-Akt-GSK-3β pathway. This work indicates a possible role of Plin5 in cerebral ischemic stroke and the up-regulation of Plin5 is a sort of survival strategy for neurons suffering from ischemic injury.
Plin5 confers neuroprotection for neurons against OGD/R damage via effects on the Nrf2-Akt-GSK-3β pathway. This work indicates a possible role of Plin5 in cerebral ischemic stroke and the up-regulation of Plin5 is a sort of survival strategy for neurons suffering from ischemic injury.
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