Notes

Impact regarding ultrasound request around the microbiota involving organic goat whole milk plus some foodstuff pathoenic agents which includes Brucella melitensis.
05). Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation, and its degradation could be associated with microbes belonging to the order Coriobacteriales. Our results suggest that multiple factors (e.g. diet and time) should be considered in animal experiments to test the effect of polyphenol or other plant extracts on rumen fermentation, methane emission and microbial composition. © 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.An analytical method for the simultaneous and reliable determination of 20 anti-gout and anti-osteoporosis pharmaceutical compounds in adulterated health food products was developed using LC-electrospray-ionization-tandem MS and LC-quadrupole-time-of-flight-MS. The method was validated through the determination of specificity, linearity, limit of detection (LOD) and quantification (LOQ), method detection limit, method quantitation limit, precision, accuracy, recovery, and stability. The matrix effect was also determined. The validation results of the developed method are as follows for solid and liquid blank samples, LODs ranged from 0.05 to 5.00 ng/mL and LOQs ranged from 0.15 to 15.00 ng/mL. Linearity was acceptable, and the correlation coefficients (R2 ) were ≥ 0.99 for all target compounds. Both intra and inter-day precision were less than 9.16% RSD, and accuracies ranged from 95.31 to 116.68%. Mean recoveries for different types of dietary supplements classified as powders, liquids, tablets, and capsules were found to be 80.81 to 117.62% with less than 15.00% RSD. The stability of the standard mixture solution was less than 11.72% RSD after 48 h. By the proposed method, the presence of dexamethasone was determined in seized herbal food products at concentrations which ranged from 126 to 215 μg/g. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.Invited for the cover of this issue is the group of Ulrich Schwaneberg at the Institute of Biotechnology, RWTH-Aachen University and DWI Lebniz Institute of Interactive Materials. The picture calls for special attention to be paid to the extra Cu binding site of Copper efflux Oxidase (CueO), due to its predominant function in tuning the electrocatalytic kinetics towards oxygen reduction. Read the full text of the article at 10.1002/chem.201905598. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.Manic-depressive illness has too often been outshined by Bipolar Disorders (BD). Young psychiatrists risk forgetting the tight link between mania and depression and regard the two poles of illness as separate entities. This approach is unsupported by historical and psychopathological standpoints. Malhi's and colleagues recently proposed ACE (Activity, Cognition and Emotion) model, which is based on Weygandt's and Kraepelin's observations (1), encourages the framing of BD in a larger perspective. This article is protected by copyright. All rights reserved.The hypothalamus and hippocampus are sensitive to early exposure to endocrine disrupting chemicals (EDCs). Two EDCs that have raised particular concerns are bisphenol A (BPA), a widely prevalent chemical in many common household items, and genistein (GEN), a phyto-oestrogen present in soy and other plants. We hypothesised that early exposure to BPA or GEN may lead to permanent effects on gene expression profiles for both coding RNAs (mRNAs) and microRNAs (miRs), which can affect the translation of mRNAs. Such EDC-induced biomolecular changes may affect behavioural and metabolic patterns. California mice (Peromyscus californicus) male and female offspring were developmentally exposed via the maternal diet to BPA (5 mg kg-1 feed weight low dose [LD] and 50 mg kg-1 feed weight upper dose [UD]), GEN (250 mg kg-1 feed weight) or a phyto-oestrogen-free diet (AIN) control. Behavioural and metabolic tests were performed at 180 days of age. A quantitative polymerase chain reacttion analysis was performed for candidate mRNAs and miRs in the hypothalamus and hippocampus. LD BPA and GEN exposed California mice offspring showed socio-communication impairments. Hypothalamic Avp, Esr1, Kiss1 and Lepr were increased in LD BPA offspring. miR-153 was elevated but miR-181a was reduced in LD BPA offspring. miR-9 and miR-153 were increased in the hippocampi of LD BPA offspring, whereas GEN decreased hippocampal miR-7a and miR-153 expression. Correlation analyses revealed neural expression of miR-153 and miR-181a was associated with socio-communication deficits in LD BPA individuals. The findings reveal a cause for concern such that developmental exposure of BPA or GEN in California mice (and potentially by translation in humans) can lead to long standing neurobehavioural consequences. © 2020 British Society for Neuroendocrinology.We report a novel synthesis of CsPbX 3 perovskite nanoplates (PNPLs) driven by SnX 4 (X = Cl, Br, I) salts and address the role played by these hard Lewis acids in directing the formation of PNPLs. Sn 4+ disturbs the acid-base equilibrium of the system, increasing the protonation rate of oleylamine and inducing an anisotropic growth of the nanocrystals. Sn 4+ cations influence the reaction dynamics due to its complexation with oleylamine molecules. By monitoring the photoluminescence excitation and photoluminescence (PL) spectra of the PNPLs grown at different synthesis temperatures, we map the influence of the thickness on their optical properties. Time and spectrally resolved PL for colloidal dispersions with different optical densities, reveals that the dependence of the overall PL lifetime on the emission wavelength do not originate from energy transfer between PNPLs, but results from the contribution of PNPLs with distinct thickness, indicating that thicker PNPLs exhibit longer PL lifetimes. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.In order to improve the poor solvent resistance and poor temperature resistance caused by traditional sizing agents, crystalline poly(ether ether ketone) (PEEK) is introduced to the interfacial phases of carbon fiber (CF) reinforced PEEK composites by a soluble precursor named PEEK-1,3-dioxolane. By changing the soluble precursor molecular weight and concentration in the sizing solution, the content of PEEK coated on the CF fiber surface can be controlled and the different interfacial properties of the PEEK composites can be obtained. The results shows that, with this method, crystalline PEEK can be completely coated on the CF surface, and the interfacial shear strength of the PEEK composites increases from 43.42 to 83.13 MPa. Due to none of any soluble compounds in the PEEK composites, the interfacial layer is well preserved under organic solvents and hygrothermal conditions, and the interfacial shear strength (IFSS) of the PEEK composites maintained above 85.4% and 90.44%, respectively. Scanning electron microscope clarifies that the mechanism of interface enhancement comes from a better wetting of crystalline PEEK on the fiber surface. Additionally, the sizing system of this investagation has the potential commercial value because of no toxic reagent (such as 2,4,5-trichloro-1-hydroxy-benzene or concentrated sulfuric acid) is required during sizing. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Cotton fiber is a kind of biodegradable material which possesses the properties such as high specific area, adjustable shape and hygroscopicity. In this work, organic polymer was directly in situ grown on the surface of cotton thread and packed into PEEK tube for online in-tube solid-phase microextraction. The novel strategy solves the problems like high backpressure and tedious optimization process of normal monolithic polymer based in-tube SPME capillary. The quaternary ammonium typed ionic liquid of 1-allyl-methylimidazolium chloride, 4-vinylbiphenyl and ethylene dimethacrylate were co-polymerized and in situ grown on the surface of cotton thread as extraction phase. The SPME tube showed excellent performance for the extraction of three non-steroidal anti-inflammatory drugs including ketoprofen, naproxen and flurbiprofen due to the strong ion exchange and hydrophobic interactions. After online coupling with HPLC system by six-port valve, the method was applied for the quantitative analysis of non-steroidal anti-inflammatory drugs in human plasma samples showing good enrichment performance (enrichment factor between 263-279), high sensitivity, good linearity and good reproducibility. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.Histone modifications play important roles in regulating chromatin dynamic changes. In this study, acetylated histone H3 lysine 9 and 18 (H3K9ac and H3K18ac), acetylated histone H4 lysine 5 and 8 (H4K5ac and H4K8ac), tri-methylation histone H3 lysine 4 (H3K4me3), di-methylation histone H3 lysine 9 (H3K9me2) are investigated in bovine oocytes, zygote, and preimplantation. During meiosis, H3K9ac and H3K18ac are erased after germinal vesicle breakdown, H4K8ac is erased after metaphase I (MI). Although H4K5ac is erased at MI, it is redetectable after this stage. However, histone methylations have no significant change during meiosis. During fertilization, intensive H4K5ac and H4K8ac are resumed on male and female chromatins at postfertilization 4 and 8 hr, respectively. H3K9ac and H3K18ac are resumed on both male and female chromatins at postfertilization 8 and 12 hr, respectively. H3K4me3 and H3K9me2 gradually increased on male chromatin after postfertilization 8 hr, while these two signals on female chromatin are detectable from postfertilization 2-18 hr. Metabolism inhibitor During embryo cleavage, H3K9ac, H3K18ac, and H3K4me3 are reduced at 8-cell stage, and then start to increase. H4K5ac, H4K8ac, and H3K9me2 increase after the 4-cell stage. At interphase, H4K5ac and H4K8ac are more intensive in nuclear periphery from 2- to 8-cell stages. During mitosis, the signal of H4K8ac is intensive at chromosome periphery. In summary, during both oocyte meiosis and fertilization, the dynamic changes of both histone acetylations and methylations happen in a process of lysine residue-specific and species-specific. During preimplantation development, the dynamic patterns of both H3K9ac and H3K18ac are similar to that of H3K4me3, while the dynamic pattern of H4K5ac is similar to that of H4K8ac. These results will be helpful for understanding the effect of histone posttranslational modifications on bovine reproduction and development. © 2020 Wiley Periodicals, Inc.Yunling cattle, a three-breed cross consisting of 1/2 Brahman cattle, 1/4 Murray Grey cattle and 1/4 Yunnan Yellow cattle, has advantage of rapid growth, good meat quality, enhanced tolerance towards a hot and humid climate, tick resistance and crude feed forbearance. Here, we investigated the genetic diversity, global and local ancestry proportions by sequencing the genomes of 131 Yungling cattle and 31 Brahman cattle (for control). Furthermore, we used 35 published genomes of ancestry breeds (including Angus cattle [ancestry of Murray Grey], Wannan cattle [ancestry of Yunnan Yellow cattle Wenshan and Dianzhong], Wenshan and Dianzhong cattle) to characterize the formation process of Yunling cattle. The highest nucleotide diversity was found in the Wannan cattle, followed by Wenshan, Dianzhong, Brahman, Yunling and Angus cattle. The results of LD decay in each breed was largely consistent with the results of nucleotide diversity, except for the faster decay in Angus cattle out of a smaller effective population size and a strong bottleneck during the breed formation.
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