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ADAM17 mediates ectodomain getting rid of of the disolveable VLDL receptor fragment in the retinal epithelium.
OBJECTIVE The apparent absence of any specific underlying diseases challenges patient-provider communication about medically unexplained symptoms (MUS). Previous research focused on general communication patterns in these interactions; however, an overview of more detailed interactional and linguistic aspects is lacking. This review aims to gain a detailed understanding of communicative challenges in MUS consultations by synthesizing evidence from conversation and discourse analytic research. METHODS A systematic review of publications using eight databases (PubMed, Embase, CINAHL, PsychINFO, Web of Science, MLA International Bibliography, LLBA and Communication Abstracts). Search terms included 'MUS', 'linguistics' and 'communication'. Additional studies were identified by contacting experts and searching bibliographies. We included linguistic and/or interactional analyses of natural patient-provider interactions about MUS. Two authors independently extracted the data, and quality appraisal was based on internal and external validity. RESULTS We identified 18 publications that met the inclusion criteria. The linguistic and interactional features of MUS consultations pertained to three dimensions 1) symptom recognition, 2) double trouble potential (i.e. patients and providers may have differing views on symptoms and differing knowledge domains), and 3) negotiation and persuasion (in terms of acceptable explanations and subsequent psychological treatment). We describe the recurrent linguistic and interactional features of these interactions. CONCLUSIONS Despite the presence of a double trouble potential in MUS consultations, validation of symptoms and subtle persuasive conduct may facilitate agreement on illness models and subsequent (psychological) treatment. Macrophages are among the first cells to interact with biomaterials and ultimately determine their integrative fate. Biomaterial surface characteristics like roughness and hydrophilicity can activate macrophages to an anti-inflammatory phenotype. Wnt signaling, a key cell proliferation and differentiation pathway, has been associated with dysregulated macrophage activity in disease. However, the role Wnt signaling plays in macrophage activation and response to biomaterials is unknown. The aim of this study was to characterize the regulation of Wnt signaling in macrophages during classical pro- and anti-inflammatory polarization and in their response to smooth, rough, and rough-hydrophilic titanium (Ti) surfaces. Peri-implant Wnt signaling in macrophage-ablated (MaFIA) mice instrumented with intramedullary Ti rods was significantly attenuated compared to untreated controls. Wnt ligand mRNA were upregulated in a surface modification-dependent manner in macrophages isolated from the surface of Ti implanted in C57Bl/6 mice. In vitro, Wnt mRNAs were regulated in primary murine bone-marrow-derived macrophages cultured on Ti in a surface modification-dependent manner. When macrophageal Wnt secretion was inhibited, macrophage sensitivity to both physical and biological stimuli was abrogated. Loss of macrophage-derived Wnts also impaired recruitment of mesenchymal stem cells and T-cells to Ti implants in vivo. Finally, inhibition of integrin signaling decreased surface-dependent upregulation of Wnt genes. These results suggest that Wnt signaling regulates macrophage response to biomaterials and that macrophages are an important source of Wnt ligands during inflammation and healing. Exosomes and extracellular nanovesicles (NV) derived from mesenchymal stem cells (MSC) may be used for the treatment of ischemic stroke owing to their multifaceted therapeutic benefits that include the induction of angiogenesis, anti-apoptosis, and anti-inflammation. However, the most serious drawback of using exosomes and NV for ischemic stroke is the poor targeting on the ischemic lesion of brain after systemic administration, thereby yielding a poor therapeutic outcome. In this study, we show that magnetic NV (MNV) derived from iron oxide nanoparticles (IONP)-harboring MSC can drastically improve the ischemic-lesion targeting and the therapeutic outcome. Because IONP stimulated expressions of therapeutic growth factors in the MSC, MNV contained greater amounts of those therapeutic molecules compared to NV derived from naive MSC. Following the systemic injection of MNV into transient middle-cerebral-artery-occlusion (MCAO)-induced rats, the magnetic navigation increased the MNV localization to the ischemic lesion by 5.1 times. The MNV injection and subsequent magnetic navigation promoted the anti-inflammatory response, angiogenesis, and anti-apoptosis in the ischemic brain lesion, thereby yielding a considerably decreased infarction volume and improved motor function. Overall, the proposed MNV approach may overcome the major drawback of the conventional MSC-exosome therapy or NV therapy for the treatment of ischemic stroke. In this work, biochar based hydrogel microspheres were fabricated successfully to develop pH and ion strength dual-stimuli responsively controlled-release system for hydrophilic pesticide. Herein, gentian violet (GV) was selected as model hydrophilic pesticide. Taking advantage of the cross-linking reaction, GV was incorporated into biochar and the 3D network-structured hydrogel, guaranteeing a satisfying encapsulation efficiency and sustained release of pesticide. The leaching behavior of pesticide in simulated soil column at different pHs and ion strength was in accordance with the corresponding release performance, and bulk of pesticide was retarded on the surface. In addition, the pesticide carrier had nearly no toxic effect on the cell proliferation and zebrafish embryo, displaying a good biosafety. The work provides a promising strategy with a low-cost and simple procedure that could regulate pesticide release behavior, decrease leaching loss, and improve the utilization efficiency of pesticide. Soil nitrogen (N) mineralization is a microbially-mediated biogeochemical process that is strongly influenced by changing climates. However, little information is available on the mechanisms behind the response of N mineralization to prolonged warming coupled with drought in soils covered by biocrusts. We used open top chambers to investigate the rate of soil N transformation (ammonification, nitrification and mineralization), enzyme activity and gene abundance in response to warming coupled with reduced precipitation over three years (2016-2018). Warming and drought significantly reduced the N transformation rate, extracellular enzyme activity, and gene abundance in moss-covered soil. For cyanobacteria-covered soil, however, it inhibited enzyme activity and increased the abundance of the nitrification-related genes and therefore nitrification rate. Our treatments had no obvious effects on N transformation and enzyme activity, but reduced gene abundance in bare soil. Biocrusts may facilitate N transformation while the degradation of moss crust caused by climate warming will dampen any regulating effect of biocrusts on the belowground microbial community. Furthermore, belowground microbial communities can mediate N transformation under ongoing warming and reduced precipitation by suppressing ammonification- and nitrification-related gene families, and by stimulating nitrification-related gene families involved in cyanobacteria-covered soil. learn more This study provides a basis for identifying the functional genes involved in key processes in the N cycle in temperate desert ecosystems, and our results further highlight the importance of different biocrusts organisms in the N cycle in temperate deserts as Earth becomes hotter and drier. V.Humic acids (HA) play an important role in the distribution, toxicity, and bioavailability of metals in the environment. Humic-like acids (HLA) that simulate geochemical processes can be prepared by NaOH aqueous extraction from hydrochars produced by hydrothermal carbonization (HTC). HLA can exhibit properties such as those found in HA from soils, which are known for their ability to interact with inorganic and organic compounds. The molecular characteristics of HLA and HA help to explain the relationship between their molecular features and their interaction with metallic species. The aim of this study is to assess the molecular features of HA extracted from Terra Mulata (TM) and HLA from hydrochars as well as their interaction with metals by using Cu(II) ions as a model. The results from 13C NMR, elemental analysis, FTIR, and UV-Vis showed that HA are composed mostly of aromatic structures and oxygenated functional groups, whereas HLA showed a mutual contribution of aromatic and aliphatic structures as main constituents. The interactions of HA and HLA with Cu(II) ions were evaluated through fluorescence quenching, in which the density of complexing sites per gram of carbon for interaction was higher for HLA than for HA. Furthermore, the HLA showed similar values for stability constants, and higher than those found for other types of HA in the literature. In addition, the average lifetime in both humic extracts appeared to be independent of the copper addition, indicating that the main mechanism of interaction was static quenching with a non-fluorescent ground-state complex formation. Therefore, the HLA showed the ability to interact with Cu(II) ions, which suggests that their application can provide a new approach for remediation of contaminated areas. V.In this work, nitrogen-doped cathodes for high H2O2 production and sulfathiazole (STZ) degradation in electro-Fenton (EF) systems were prepared by the carbonization of three carbon/nitrogen-enriched precursors. Among the cathodes elaborated from different precursors, the one using 1h-1,2,4-triazole-3,5-diamine as the precursor showed the best oxygen reduction reaction (ORR) ability with the normalized H2O2 accumulation of 9.49 ± 0.03 mg L-1 h-1 cm-2 compared to the other two N-containing cathodes. The enhanced H2O2 accumulation was attributed to the high electroactive surface area and pyrrolic N (60.45%) content. Regarding reactive oxygen species in the absence of Fe2+, aside from the H2O2, O2-and 1O2 were identified using spectroscopic techniques and chemical probes. As a result, a degradation and mineralization efficiency of 98.25 ± 0.14% and 70.57 ± 0.27% of STZ were attained in the 180-min treatment, mainly coming from the homogeneous OH from classical Fenton, anodic OH on BDD anode and direct/indirect oxidation of O2-and 1O2. In addition, the plausible degradation pathway of STZ was proposed based on the density functional theory (DFT) combined with experimental data derived by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The frontier orbital theory and Fukui function theoretically suggested the vulnerable sites of STZ for different active species including OH, O2- and 1O2. This study provides a new strategy for improving the ORR process and analyzing the generation and conversion of reactive oxygen species in the EF process. Pollution of the seas due to plastic litter is a rapidly growing environmental problem. Among several actions, legal and technological, undertaken to alleviate this problem, included are the control of single use conventional plastics and the replacement of conventional non-biodegradable plastics with innovative biodegradable-in-the-sea polymers, both aiming at the mitigation of marine litter accumulation. Laboratory tests have been used to characterize plastics regarding their biodegradation in various environments. Biodegradation of plastics depends on the inherent characteristics of the polymer and the particular marine habitat conditions. In the present work, the international standard test method ISO 19679 (2016) for determining the aerobic biodegradation of non-floating plastic materials in a seawater-sediment interface of the coastal marine zone under laboratory simulated conditions is evaluated. Modifications are proposed to improve the reliability of this test method in some aspects. Agitation of the seawater surface in the bioreactor was found to enhance the continuous availability of oxygen at the seawater-sediment interface, thus assuring aerobic biodegradation conditions throughout the test simulating real sublittoral conditions.
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