Notes

Identifying Return to Activity: A deliberate Assessment.
Considering the extensive production and continuous usage of organophosphate esters (OPEs) around the urbanized coasts in recent years, the inter-annual variation and the ecological risks of OPEs in the Yellow Sea (YS), China were systematically evaluated in summer 2015 and 2019. The major component of OPEs, halogenated-OPEs in 78 surface and bottom seawater samples collected from 39 locations in the YS in 2019 were compared with 62 samples at 31 sites conducted in the same periods and similar stations in 2015. The average concentration of halogenated-OPEs increased 1.57, 2.50 folds in the surface and bottom seawater, respectively, in recent 5 years. The proportion of TCPP to halogenated-OPEs exhibited an increasing trend from 56.49% in 2015 to 79.95% in 2019. The peak values of halogenated-OPEs changed from the nearshore regions (33°N-38°N) in 2015 to the nearshore and offshore regions (33°N-35°N) in 2019, implying the growing terrigenous emission sources, especially some large OPEs producers nearby the coastal zones in the northern area of Jiangsu province. The targeted halogenated-OPEs posed no significant or low ecological risks to the aquatic organisms in 2015 and 2019, however, the values of RQ showed an elevated trend in 2019. Therefore, the ecological risks of OPEs in this region still need continuous concern due to the gradually increasing OPEs usage and production.Macroalgae and their rocky substrates both support diverse and abundant microbiota, performing essential ecological functions in marine ecosystem. However, the differences in the epiphytic microbial communities on macroalgae and rocky substrate are still poorly understood. In this study, the epiphytic microbial communities on four macroalgae (Corallina officinalis, Rhodomela confervoides, Sargassum thunbergii, and Ulva linza) and their rocky substrates from Weihai coast zone were characterized using high-throughput sequencing technology. The results showed that the alpha diversity indices were greater in rocky substrates than that in macroalgae. The microbial similarities among macroalgae and rocky substrate groups tended to decrease from the high taxonomic ranks to lower ranks, only 22.69% of the total amplicon sequence variants (ASVs) were shared between them. The functional analysis revealed that the microbiotas were mainly involved in metabolic activities. This study would provide the theoretical foundation for macroalgal cultivation and algal reef applications.Krossfjord-Kongsfjord system in the European Arctic has been assessed for abundance, source and distribution of microplastics in the surface sediments. The average abundance of microplastics in Krossfjord and Kongsfjord is 721.42 ± 217.89 (n = 5) pieces/kg and 783 ± 530.28 (n = 8) pieces/kg. Polymers like polyethylene and polypropylene are abundant in the sediment samples. Fibers are the most common shape of plastic particles. A high abundance of smaller plastic particles in the sediment may be due to the fragmentation of larger plastic particles during transportation. The microplastics' spatial distribution, shape, size, and composition suggest that the long-range transport by west Spitsbergen current and local inputs from anthropogenic activities are possible sources of microplastics found in the study area. Our results exhibit the presence of microplastic pollution, suggesting the influence of anthropogenic activity in the Arctic fjord and the need to control/reduce marine pollution which has become a potential threat to marine organisms.Electroactive biofilms have attracted increasing attention due to their unique ability to exchange electrons with electrodes. Geobacter spp. are widely found to be dominant in biofilms in acetate-rich environments when an appropriate voltage is applied, but it is still largely unknown how these bacteria are selectively enriched. Herein, two key Geobacter spp. that have been demonstrated predominant in wastewater-enriched electroactive biofilm after long-term operation, G. sulfurreducens and G. anodireducens, responded to electric field (EF) differently, leading to a higher abundance of EF-sensitive G. anodireducens in the strong EF region after cocultivation with G. sulfurreducens. Transcriptome analysis indicated that two-component systems containing sensor histidine kinases and response regulators were the key for EF sensing in G. anodireducens rather than in G. sulfurreducens, which are closely connected to chemotaxis, c-di-GMP, fatty acid metabolism, pilus, oxidative phosphorylation and transcription, resulting in an increase in extracellular polymeric substance secretion and rapid cell proliferation. Our data reveal the mechanism by which EF select specific Geobacter spp. over time, providing new insights into Geobacter biofilm formation regulated by electricity.A comprehensive real-time evaluation of the chemical status of surface water bodies is still utopian, but in our opinion, it is time to use the momentum delivered by recent advanced technical, infrastructural, and societal developments to get significantly closer. Procedures like inline and online analysis (in situ or in a bypass) with close to real-time analysis and data provision are already available in several industrial sectors. In contrast, atline and offline analysis involving manual sampling and time-decoupled analysis in the laboratory is still common practice in aqueous environmental monitoring. Methylation inhibitor Automated tools for data analysis, verification, and evaluation are changing significantly, becoming more powerful with increasing degrees of automation and the introduction of self-learning systems. In addition, the amount of available data will most likely in near future be increased by societal awareness for water quality and by citizen science. In this analysis, we highlight the significant potential of surface water monitoring techniques, showcase "lighthouse" projects from different sectors, and pin-point gaps we must overcome to strike a path to the future of chemical monitoring of inland surface waters.Drinking-water treatment in non-networked rural communities relies on the use of point-of-use (PoU) household filters. Source waters treated by PoU filters are often microbially contaminated, but information about human enteric virus reductions in these filters is limited. This study evaluated human rotavirus, adenovirus and norovirus reductions in 10 commonly used, new PoU carbon, polypropylene and polyester microfilters. The viruses were spiked into chlorine-free tap water (pH 8.0, ionic strength 1.22 mM), and 3 sequential challenge tests were conducted in each filter under a constant flow rate of 1 L/min. In most of the filters investigated, the norovirus and adenovirus reductions were similar (P > 0.49). Compared with the norovirus and adenovirus reductions, the rotavirus reductions were significantly lower in the carbon filters (P ≤ 0.009), which may relate to rotavirus's higher zeta potential and lower hydrophobicity. Virus reductions appeared to be dictated by the filter media type through electrostatic and hydrophobic interactions; the effects of filter media pore sizes on virus reductions via physical size-exclusion were very limited. The virus reductions in the carbon filters were significantly greater than those in the polypropylene and polyester filters (P ≤ 0.0001), and they did not differ significantly between the polypropylene and polyester filters (P > 0.24). None of the filters met the "protective" rotavirus reduction level (≥3 log10) required for household drinking-water treatment. Our study's findings highlight a critical need for additional water treatment when using PoU microfilters, for example, water boiling or ultraviolet radiation, or the use of effective surface-modified filter media to prevent drinking-waterborne infections from enteric viruses.Wastewater treatment carried out by microalgae is usually affected by the type of algal strain and the combination of cultivation parameters provided during the process. Every microalga strain has a different tolerance level towards cultivation parameters, including temperature, pH, light intensity, CO2 content, initial inoculum level, pretreatment method, reactor type and nutrient concentration in wastewater. Therefore, it is vital to supply the right combination of cultivation parameters to increase the wastewater treatment efficiency and biomass productivity of different microalgae classes. In the current investigation, the decision tree was used to analyse the dataset of class Trebouxiophyceae and Chlorophyceae. Various combinations of cultivation parameters were determined to enhance their performance in wastewater treatment. Nine combinations of cultivation parameters leading to high biomass production and eleven combinations each for high nitrogen removal efficiency and high phosphorus removal efficiency for class Trebouxiophyceae were detected by decision tree models. Similarly, eleven combinations for high biomass production, nine for high nitrogen removal efficiency, and eight for high phosphorus removal efficiency were detected for class Chlorophyceae. The results obtained through decision tree analysis can provide the optimum conditions of cultivation parameters, saving time in designing new experiments for treating wastewater at a large scale.Timely and simultaneously detecting BOD and nitrite concentrations is of great significance for curbing of water pollution and adjusting wastewater treatment strategies. However, existing BOD and nitrite biosensors cannot perform synchronous detection due to their single electroactivity and differences in detection time. This study reported a novel dual-function electrochemical biosensor (DFEB) that could perform fast, simultaneous detection of nitrite and dissolved BOD. DFEB conducted a potential-step chronoamperometry on the mixed-bacteria bioelectrode with bidirectional electron transfer ability to obtain response signals. DFEB accurately measured dissolved BOD in the range of 5 ∼ 100 mg BOD L-1 and nitrite in the range of 0.05 ∼ 16 mg NO2--N L-1 within 20 min and maintain stable performance over 200 tests. DFEB performed well in artificial wastewater, aquatic wastewater, anaerobic tank effluent and anammox effluent, with relative errors less then 15.7% and 16.8% in detecting nitrite and dissolved BOD, respectively. Our study provided a feasible way to develop multifunctional biosensors for detecting pollutants with different redox properties in wastewater.
|The aim of this study was to evaluate the use of volumetric HRCT measurements in the diagnosis of enlarged vestibular aqueduct syndrome (EVAS) and describe the association of this novel radiographic approach with clinical hearing outcomes. We hypothesized that volumetric measurements may have stronger correlation to hearing loss given the anatomic variability of the vestibular aqueduct in linear measurements.

A retrospective study design was used, including 51 patients that fit the inclusion criteria for the study for a total of 81 ears. 3D volumes were calculated using the MIM Software platform (MIM Software Inc.) from semiautomatic segmentation of the VA across individual slices on CT scan. Air and bone conduction data was collected from medical records with the air-bone gap being calculated from these data. Univariate and multivariate analyses were conducted to determine if volumetric VA size correlated with hearing loss outcomes.

Out of the study population, 30 subjects (58.8%) demonstrated bilateral EVA.
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