Notes

Knockdown associated with lncRNA HIF1A-AS2 raises substance level of sensitivity associated with SCLC cells in colaboration with autophagy.
Gelatin is one of the most versatile biopolymers in various biomedical applications. A gelatin derivative gelatin-catechol (Gel-C) was developed in this study to further optimize its chemical and physical properties such as thermal reversibility and injectability. We found that Gel-C remains in a solution state at room temperature, and the temperature-dependent gelation capability of gelatin is well preserved in Gel-C. Its gel-forming temperature decreased to about 10 °C (about 30 °C for gelatin), and a series of gelatin derivatives with different gel-forming temperatures (10-30 °C) were formed by mixing gelatin and Gel-C in different ratios. Additionally, irreversible Gel-C hydrogels could be made without the addition of external stimuli by combining the physical cross-linking of gelatin and the chemical cross-linking of catechol. At the same time, properties of Gel-C hydrogels such as thermal reversibility and injectability could be manipulated by controlling the temperature and pH of the precursor solution. By simulating the formation of an irreversible Gel-C hydrogel in vivo, an in situ gelling system was fabricated by lowering the local temperature of the hydrogel with cold shock, thus realizing targeted and localized molecular delivery with prolonged retention time. This simple system integrated with the temperature responsiveness of gelatin and chemical cross-linking of catechol groups thus provides a promising platform to fabricate an in situ gelling system for drug delivery.Oil spills in the Arctic have drawn dramatic attention in recent years. Frazil ice, as the essential formation of sea ice, may affect the effectiveness of dispersants during oil spill response and the associated behaviors of dispersed oil. However, these impacts remain poorly understood, limiting the appropriate usage of dispersants in ice-covered regions. Herein this work explored the effects of frazil ice on the dispersion effectiveness of two dispersants (Corexit 9500A and hydrolyzed shrimp waste) and the migration of dispersed oil within frazil ice. We discovered that frazil ice inhibited dispersion effectiveness by attenuating water velocity. Permeable frazil ice encapsulated 11-30% of dispersed oil, implying a lower oil bioavailability. We thus proposed and verified a microscopic mechanism to unravel the migration of dispersed oil toward permeable constrictions in frazil ice. We predicted the concentration of dispersed oil encapsulated in frazil ice using bed filtration theory and verified the prediction through experiments. Furthermore, the presence of frazil ice can lead to the breakup and coalescence of dispersed oil. Overall, our findings would facilitate the appropriate planning and decision-making of dispersant-based oil spill response and a better understanding of the fate of dispersed oil in the frazil ice-infested ocean.Polycyclic aromatic hydrocarbons (PAHs) are a class of the most hazardous substances. As unavoidable byproducts of petrogenic and pyrogenic processes, their emissions are dominantly linked to various economic sectors. In international trade, not only final consumption but also primary input can transfer the emissions among regions. Therefore, a long-term impact assessment of the international trade on PAH global emissions based on the final consumption and primary input could significantly benefit worldwide PAH mitigation strategies. This study investigated the changes in consumption- and income-based PAH emissions and interregional flows of worldwide regions, using the latest available data from 1999 to 2014. Results show that in 2014, 16.8 and 10.1% of global PAH emissions were transferred by consumption and primary input through international trade. Meanwhile, the production-, consumption-, and income-based emissions in most regions were decreasing. Furthermore, from the consumption-based perspective, sub-Saharan Africa surpassed China and became the largest net exporter of consumption-based emissions. From the income-based perspective, the net income-based outflows of India and the rest of Asia increased significantly, indicating the income-based emission leakage in emerging markets. From the socioeconomic perspective, emission intensity dominated the global decline in PAH emissions. As the two main factors driving the increase in emissions, the primary input structure (41%) had a larger effect than the final demand level (28%) from 1999 to 2014. Therefore, global cooperation, through the mitigation strategies of reducing emission factors and improving international trade patterns, is posited as an efficient strategy to reduce PAH pollution and related health risks.Trions, quasiparticles composed of an electron-hole pair bound to a second electron and/or hole, are many-body states with potential applications in optoelectronics. Trions in monolayer transition metal dichalcogenide (TMD) semiconductors have attracted recent interest due to their valley/spin polarization, strong binding energy, and tunability through external gate control. However, low materials quality (i.e., high defect density) has hindered efforts to understand the intrinsic properties of trions. The low photoluminescence (PL) quantum yield (QY) and short lifetime of trions have prevented harnessing them in device applications. Here, we study the behavior of trions in a series of MoSe2 monolayers, with atomic defect density varying by over 2 orders of magnitude. The QY increases with decreasing defect density and approaches unity in the cleanest material. Simultaneous measurement of the PL lifetime yields both the intrinsic radiative lifetime and the defect-dependent nonradiative lifetime. The long lifetime of ∼230 ps of trions allows direct observation of their diffusion.Semicarbazide (SEM) is a widespread carcinogenic and neurotoxic food contaminant, originating from the metabolite of antibiotic nitrofurazone, which is used in aquaculture, or thermal decomposition byproduct of a flour blowing agent azodicarbonamide. Although optical detection technologies are powerful tools considering the advantages of fast response and visualization detection, there are few optical nanosensors for highly sensitive and visual assays of SEM due to no luminescence response and UV absorbance of SEM. Herein, an upconversion luminescence (UCL)-based nanosensor was designed for visual detection of SEM with high sensitivity and good selectivity. The nanosensor was constructed by combining upconversion nanoparticles (UCNPs) and phosphomolybdic acid (PMA), which was used as the specific recognition element of SEM. The developed nanosensor exhibited selective absorbance enhancement and UCL quenching behavior with the addition of SEM based on the inner filter effect (IFE). Since the change in absorbance translated into an exponential change in the luminescence, the sensitivity of the nanosensor was greatly improved. The nanosensor realized a highly sensitive and visual response to SEM in the linear range of 0.5-16 μM with a low limit of detection of 58 nM. Moreover, satisfactory recovery values ranging from 90 to 112% in spiked real samples indicated the practical applicability of the nanosensor. The nanosensor designed here provides a sensitive and convenient sensing strategy for visual detection of hazardous substances and is expected to develop the upconversion sensing application in food safety.Silver-based nano-antibiotics are rapidly developing as promising alternatives to conventional antibiotics. Ideally, to remain potent against a wide range of drug-resistant and anaerobic bacteria, silver-based nano-antibiotics should easily penetrate through the bacterial cell walls and actively release silver ions. In this study, highly monodispersed, ultrasmall (50%) of silver ions (i.e., Ag+ nanoreservoirs) on the cluster surface maintains their efficiency in both aerobic and anaerobic conditions. PPAR agonist Significantly, the pAgNCs showed a strong capacity to significantly delay the development of bacterial resistance when compared to similar-sized negatively charged silver nanoparticles or conventional antibiotics. This study demonstrates a novel design strategy that can lay the foundation for the development of future highly potent nano-antibiotics effective against a broad spectrum of pathogens and biofilms needed in many everyday life applications and industries.Engineered small extracellular vesicles (sEVs) are used as tools to enhance therapeutic efficacy. However, such application of sEVs is associated with several issues, including high costs and a high risk of tumorigenesis. Nanotopography has a greater influence on bone-related cell behaviors. However, whether nanotopography specifically mediate sEV content to perform particular biological functions remains unclear. Here, we demonstrate that selective nanotopography may be used to sequentially mediate human bone mesenchymal stem cell (hBMSC) sEVs to enhance the therapeutic efficacy of hBMSCs-EVs for osteogenesis. We subjected sEVs harvested from hBMSCs cultured on polished titanium plates (Ti) or nanotopographical titanium plates (Ti4) after 7, 14, and 21 d for RNA sequencing, and we found that there was no significant difference in sEV-miRNA expression after 7 d. Differentially expressed osteogenic-related microRNAs were founded after 14 days, and KEGG analysis indicated that the main microRNAs were associated with osteogenesis-related pathways, such as TGF-beta, AMPK, and FoxO. A significant difference was found in sEV-miRNAs expression after 21 d. We loaded sEV secreted from hBMSCs cultured on Ti4 after 21 d on 3D-printed porous PEEK scaffolds with poly dopamine (PDA) and found that such scaffolds showed superior osteogenic ability after 6- and 12-weeks. Here, we demonstrate the alkali- and heat-treated nanotopography with the ability of stimulating osteogenic differentiation of hBMSC can induce the secretion of pro-osteogenesis sEV, and we also found that sEVs meditate osteogenesis through miRNA. Thus, whether nanotopography has the ability to regulate other contents of sEVs such as proteins for enhancing osteogenesis needs further research. These findings may help us use nanotopography to extract sEVs for other biomedical applications, including cancer therapy.In recent years, dozens of halogenated disinfection byproducts (DBPs) with cyclic structures were identified and detected in drinking water globally. Previous in vivo toxicity studies have shown that a few new cyclic DBPs possessed higher developmental toxicity and growth inhibition rate than common aliphatic DBPs; however, in vitro toxicity studies have proved that the latter exhibited higher cytotoxicity and genotoxicity than the former. Thus, to provide a more comprehensive toxicity comparison of DBPs from different endpoints, 11 groups of cyclic DBPs and nine groups of aliphatic DBPs were evaluated for their comparative in vitro and in vivo toxicity using human hepatoma cells (Hep G2) and zebrafish embryos. Notably, results showed that the in vitro Hep G2 cytotoxicity index of the aliphatic DBPs was nearly eight times higher than that of the cyclic DBPs, whereas the in vivo zebrafish embryo developmental/acute toxicity indexes of the cyclic DBPs were roughly 48-50 times higher than those of the aliphatic DBPs, indicating that the toxicity rank order differed when different endpoints were applied.
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