Notes

Overexpressed circRANBP17 acts as an oncogene for you to help nasopharyngeal carcinoma via the miR-635/RUNX2 axis.
ew channel abutments can effectively improve the implant stability in early phase, with high success rate, good short-term effect and few complications.
Full-zirconia single-tooth molar implant-supported restorations with angulated screw channel abutments can effectively improve the implant stability in early phase, with high success rate, good short-term effect and few complications.
To analyze the oral health behaviors of disabled children and their parents' oral health knowledge, attitudes in Shanghai city, and to provide information support for designing oral health care programs and making relevant policies.

By using stratified cluster sampling method, a questionnaire was given to 1381 parents of disabled children. The data were analyzed using SPSS 21.0 software package.

The awareness rate of oral health knowledge among parents of disabled children was 67.21%, and 78.98% of parents had positive attitudes towards oral health. 13.61% of disabled children took sweet snacks before sleep, only 45.98% of disabled children brushed their teeth twice or more daily. 42.65% of disabled children used fluoride toothpaste, and 88.12% never flossed their teeth. The percentage of disabled children who had never visited a dentist was 49.75%.

Oral health behaviors of disabled children in Shanghai city need to be improved, and the parents' oral health knowledge level is low. Customized educational programs should be carried out for parents, in order to strengthen oral health education in the suburban areas.
Oral health behaviors of disabled children in Shanghai city need to be improved, and the parents' oral health knowledge level is low. Customized educational programs should be carried out for parents, in order to strengthen oral health education in the suburban areas.
To analyze the effect of triple antibiotic paste on root canal microorganisms in periapical periodontitis of different stages.

Eighty-nine children with periapical periodontitis of deciduous teeth in Department of Stomatology, Children's Hospital of Nanjing Medical University from April 2017 to April 2020 were enrolled, and divided into two groups according to the clinical symptoms and root X-ray films, i.e., acute inflammation group and chronic inflammation group. Samples of infected root canals were collected for bacterial identification, isolation and purification. The detection of microorganisms in the infected root canal and the composition of anaerobic microorganisms were analyzed in both groups. Disk diffusion method was used to observe antimicrobial effects of triple antibiotic paste and calcium hydroxide against common anaerobic bacteria, and the sensitivity of different anaerobic bacteria to triple antibiotic paste. Statistical analysis was completed by SPSS 20.0 software package.

The microorgic paste was highest in Enterococcus faecalis, followed by Peptostreptococcus, Porphyromonas gingivalis, Fusobacterium nucleatum, and Bacteroidetes(P<0.05).

Triple antibiotic paste has good antimicrobial effect on the common bacteria in the infected root canal of acute and chronic periapical periodontitis.
Triple antibiotic paste has good antimicrobial effect on the common bacteria in the infected root canal of acute and chronic periapical periodontitis.
To explore the effect of enamel matrix proteins(EMPs) on osteogenesis and adipogenesis of stem cells from human exfoliated deciduous teeth SHED), and explore its molecular mechanism.

SHEDs were used to detect the expression of its surface antigens CD73, CD146, CD34 and CD45 by flow cytometry. SHED was induced by OB osteogenic induction liquid, and then the osteogenic differentiation ability was measured by alizarin red staining. SHEDs were divided into 4 groups, NC group had invalid sequence shRNA interfered with SHED, EMPs group had invalid sequence shRNA interfered with SHED. Then 100 μg/L EMPs was used to interfere with SHED. In miR-32 inhibitor group, miR-32 shRNA plasmid was used to interfere with SHED; while in EMPs+miR-32 inhibitor group, 100 μg/L EMP was used to intervene SHED after silencing miR-32. QPCR was used to detect the expression of miR-32, dentin sialophosphoprotein (DSPP), dentin matrix protein 1, DMP-1, peroxisome proliferators-activated receptor γ (PPARγ) and CCAAT enhancer binding pr significantly increased(P<0.05), the expression of PPARγ and C/EBPa and the number of lipid droplets were significantly decreased (P<0.05), while the result of miR-32 inhibitor group was the opposite (P<0.05). Compared with miR-32 inhibitor group, there was no significant difference in the expression of DSPP, DMP-1, PPARγ and C/EBPα, number of mineralized nodules and oil droplets in EMPs+miR-32 inhibitor group(P>0.05). Compared with EMPs group, the expression of DSPP and DMP-1 and the number of mineralized nodules in EMPs+miR-32 inhibitor group were significantly reduced(P<0.05), while the expression of PPARγ and C/EBPα and the number of lipid droplets were significantly increased(P<0.05).

EMPs can regulate osteogenic and adipogenic differentiation of SHED by promoting the expression of miR-32.
EMPs can regulate osteogenic and adipogenic differentiation of SHED by promoting the expression of miR-32.
To explore the effects of high-concentration fluoride(F) on apoptosis of human periodontal ligament stem cells (PDLSCs).

PDLSCs were isolated from periodontal ligament tissues of extracted third molars, and treated with different concentrations (0-40 ppm F) of NaF for indicated period of time. CCK-8 assay was performed to detect cell viability. After stained with Annexin V-PI and JC-1, cell apoptosis and mitochondrial membrane potential were analyzed by flow cytometry. Immunofluorescence staining and confocal microscopic assay were used to detect the protein expression level of cyt-c, cleaved-caspase-9 and -3. The mRNA level of caspase -9 and -3 were examined by RT-PCR. The protein expression level of total and phosphate-ERK, JNK and p38 were analyzed by Western blot. SPSS 13.0 software package was used for statistical analysis.

Fluoride treatment inhibited cell viability (CCK-8 assay) and induced apoptosis of PDLSCs (Annexin V-PI staining) in a dose- and time-dependent manner. Immunofluorescence assay showed that fluoride with a dose ≥10 ppm significantly induced release of cyt-c from the mitochondria to cytosol, and up-regulation of expression of cleaved-caspase -9 and -3. RT-PCR confirmed that the mRNA level of caspase-9 and -3 increased with the dose of fluoride. Western blot assay confirmed that fluoride induced up-regulation of p-ERK, but not that of p-JNK and p-p38, and specifically blocking ERK pathway with U0126 could partially rescue the fluoride-induced cell apoptosis.

High concentrations of fluoride induces apoptosis of PDLSCs via intrinsic mitochondrial pathway, and phosphation of MAPK/ERK is involved in the F-induce cell apoptosis.
High concentrations of fluoride induces apoptosis of PDLSCs via intrinsic mitochondrial pathway, and phosphation of MAPK/ERK is involved in the F-induce cell apoptosis.
To detect the effect of DKK1 on biological behaviors of human dental pulp cells exposed to lipopolysaccharide (LPS).

The dental pulp cells were isolated and cultured by modified enzyme-tissue block method and identified by immunofluorescence staining. The effect of DKK1 on proliferation and migration of human dental pulp cells exposed to LPS were measured by cell counting kit (CCK-8) and Transwell assay. Meanwhile, the effect of DKK1 on differentiation of human dental cells exposed to LPS were studied by alizarin red staining and real-time PCR experiment, statistical analysis was performed using SPSS 20.0 software package.

The results of immunofluorescence showed that the cultured cells were in consistent with the mesenchymal stem cells. The result of CCK-8 indicated that DKK1 had no significant effect on proliferation of dental pulp cells exposed to LPS; The result of transwell assay showed that DKK1 significantly promoted the cell migration of dental pulp cells exposed to LPS. The results of Alizarin red staining and real-time PCR revealed that DKK1 could promote cytodifferentiation of dental pulp cells exposed to LPS.

DKK1 promotes the ability of cell migration and cytodifferentiation of LPS treated dental pulp cells, which may be resulted from inhibition of Wnt/β-catenin pathway.
DKK1 promotes the ability of cell migration and cytodifferentiation of LPS treated dental pulp cells, which may be resulted from inhibition of Wnt/β-catenin pathway.
Porphyromonas endodontalis (P.e) is the dominant bacterium in the infected canal of pulpal and periapical disease.Lipopolysaccharides (LPS) in the outer membrane of the cell wall is an important toxicity factor of P.e. In this study, the effect of P.e-LPS on osteoblast differentiation was studied, and the pathogenic mechanism of P.e-LPS in periapical bone resorption disease was explored.

Porphyromonas endodontalis was cultured under anaerobic conditions. GSK343 price P.e-LPS was extracted by thermophenol water method, and then the extracted LPS was qualitatively analyzed by gel limulireagent method. Preosteoblast cell line MC3T3-E1 were induced to differentiate into osteoblasts by osteoblast differentiation medium (50 μg/mL ascorbic acid，6 mmol/L beta-glycerphosphate). Expressions of osteogenic differentiation genes including distal-less homeobox 5（DLX5）, runt-related transcription factor 2(Runx2), Osterix, bone sialoprotein (BSP), OCN(osteocalcin) and Collagen were detected by RT-PCR. The activity of alkaline phosphaits the differentiation of osteoblasts through TLR-4 receptor, thus participating in bone resorption process of periapical lesions.
P.e-LPS inhibits the differentiation of osteoblasts through TLR-4 receptor, thus participating in bone resorption process of periapical lesions.
To investigate the osteogenic effect of nano-grade pearl powder(NPP)/chitosan-hyaluronic acid (C-HA)/recombinant human bone morphology protein-2 (rhBMP-2) artificial bone.

A bone defect model with a diameter of 7 mm and a height of 10 mm was made at the distal end of the femur. NPP/C-HA stent containing rhBMP-2 was prepared according to the shape of the defect. No material was implanted in the defect as blank group. NPP/C-HA was used as the control group, NPP/C-HA/rhBMP-2 was implanted into the experimental group. At 4 weeks, 8 weeks, and 12 weeks, the bone effects of each component were detected by cone-beam CT(CBCT), H-E and Masson staining. Serum ALP activity and OCN in tissues to determine the osteogenic differentiation and osteogenesis maturity were detected. SPSS 18.0 software package was used for statistical analysis.

At 12 weeks, the defect was completely repaired in the experimental group. No immunological side effects such as inflammation and rejection were observed. At 8 and 12 weeks, CBCT showed that the experimental group had a higher CT value (Hounsfield units, HU) compared with the control group and the blank group(P<0.05). H-E and Masson staining showed that the experimental group had obvious new bone formation compared with the control group and the blank group at 8 weeks and 12 weeks, and ALP activity of the experimental group was significantly different from the control group and the blank group at 8 weeks. OCN immunohistochemical scoring of the experimental group was significantly different from the control group and the blank group(P<0.05).

NPP/C-HA/rhBMP-2 has good tissue fusion, osteoinductivity, osteoconductivity and osteogenicity, which is expected to provide more effective treatment for bone repair.
NPP/C-HA/rhBMP-2 has good tissue fusion, osteoinductivity, osteoconductivity and osteogenicity, which is expected to provide more effective treatment for bone repair.
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