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Microbial Diversity associated with Terrestrial Geothermal power Spgs in Armenia along with Nagorno-Karabakh: An overview.
The interest in the therapeutic values of natural compounds from plants is growing worldwide because the development of modern synthetic drugs has not lived up to expectations. The tree Ficus religiosa native to India, China and Southeast Asia is traditionally used for curing almost 50 ailments, although the majority of the individual active compounds are not known. Hence, a hyphenated high-performance thin-layer chromatography (HPTLC) method was newly developed. It allowed a physicochemical, but especially effect-directed profiling of individual compounds present in Ficus religiosa leaves obtained from four locations (in India and Germany). Extracts of different polarities were screened for bioactivity responses and most bioactivities were found in the ethyl acetate extracts. A multi-imaging via 26 different detection modes was performed, i. e. UV/Vis/FLD, 11 microchemical derivatizations and 12 effect-directed assays (EDA). By HPTLC-UV/Vis/FLD-EDA, antibiotics against Gram-positive and Gram-negative bacteria as well as acetylcholinesterase, butyrylcholinesterase, tyrosinase, α-amylase, α-glucosidase and β-glucosidase inhibitors and radical scavenging compounds were detected. Estrogen-like or gentotoxic compounds were not detected at higher extract amounts of even 5 mg/band applied. For further characterization of three most important, multipotent, bioactive compound zones, HPTLC was hyphenated with heated electrospray ionization high-resolution mass spectrometry including fragmentation (HPTLC-HESI-HRMS/MS). Multipotent bioactive compounds discovered in the extracts were equivalently calculated in reference to well-known reference inhibitors.The goal of this work was to provide a technical solution for the automated optimization of multi-column systems for protein separation and fractionation. Both algorithm and a software that can be downloaded are provided. In this algorithm, the length and order of the individual column segments can be considered. Various solutions are provided by the algorithm, including i) to obtain uniform peak distribution, ii) to park the different species at the inlet of the individual column segments, and iii) to elute all species as a single peak. Two representative examples are presented, showing the possibility to obtain uniform selectivity between monoclonal antibody (mAb) sub-units, and the on-column fractioning of intact mAbs.Synthesised polypyrrole-coated Fe3O4 magnetic nanoparticles have been successfully characterised and applied as sorbent for the magnetic-micro-dispersive solid-phase extraction of eleven phthalic acid esters from jelly and apple-based beverage matrices widely consumed by the population and, especially, by children. Sorbent was synthesised through chemical coprecipitation and subsequently characterised by different techniques. The influence of several parameters on the extraction efficiency was exhaustively evaluated using a step-by-step strategy. The separation and quantification of the selected phthalates were performed by ultra-high performance liquid chromatography coupled to tandem mass spectrometry. The validation of the methodology was carried out for jellies and apple-based beverages, employing dihexyl phthalate-3,4,5,6-d4 as the surrogate standard. Relative recovery values were in the range 70-114% for both matrices and relative standard deviations below 20% were obtained. read more The limits of quantification of the method were found in the range 0.147-0.416 µg/L. Feasibility of the developed methodology was proved by the analysis of commercialised jelly and apple-based beverage products.The efficient depolymerization and hydrodeoxygenation of enzymatic hydrolysis lignin are achieved in cyclohexane solvents over a gamma-alumina supported nickel molybdenum alloy catalyst in a single step. Under initial 3 MPa hydrogen at 320 °C, the highest overall cycloalkane yield of 104.4 mg/g enzymatic hydrolysis lignin with 44.4 wt% selectivity of ethyl-cyclohexane was obtained. The reaction atmosphere and temperature have significant effects on enzymatic hydrolysis lignin conversion, product type and distribution. The conversion of enzymatic hydrolysis lignin was also investigated over different nickel and molybdenum-based catalysts, and the gamma-alumina supported nickel molybdenum alloy catalyst exhibited the highest activity among those catalysts. To reveal the reaction pathways of alkylphenol hydrodeoxygenation, 4-ethylphenol was tested as a model compound. Complete conversion of 4-ethylphenol into cycloalkanes was achieved. A two-step mechanism of 4-ethylphenol dihydroxylation - hydrogenation is proposed, in which the benzene ring saturation is deemed as the rate-determining step.Conversion of organic wastes to fatty acids rather than methane through anaerobic digestion-based technologies has considerable promise. However, the relationships between microbiome structure and fatty acids produced from cellulosic feedstocks are not well understood. This study investigated the nature of those relationships for anaerobic digester sludge, bison rumen, and cattle rumen inocula grown on cellulose. Acetic acid production was highest in anaerobic sludge reactors, while propionic acid production was highest in cattle rumen reactors. Butyric and pentanoic acid were produced at the highest rates in bison rumen before Day 5. Reactor microbiomes remained distinct, despite identical operating conditions. Novel associations linked Alistipes with butyric acid production and Eubacterium nodatum and Clostridiales bacterium with pentanoic acid production. This study provides new insights into the ability of microbiomes to convert cellulose to different fatty acid mixtures and adds impetus for the rewiring of anaerobic digestion to generate high-value products.
This study was aimed to assess the outcome of radiotherapy and determine prognostic factors for survival in breast cancer patients with clinically overt metastasis to the internal mammary lymph node (IMN+).

We retrospectively reviewed the medical records of 193 patients with IMN+breast cancer who received neoadjuvant chemotherapy (NAC), breast surgery without internal mammary lymph node (IMN) dissection, and postoperative radiotherapy at 9 hospitals between 2009 and 2013. Breast-conserving surgery or mastectomy was performed after taxane-based NAC. Radiotherapy was administered to the whole breast/chest wall and regional nodes. IMN-covering radiotherapy was performed in 92.2% of patients with median dose of 58.4Gy (range, 44.9-69.1Gy). The overall survival (OS), disease-free survival (DFS), and IMN failure-free survival (IMNFFS) were analyzed.

After median follow-up of 71 months, 9 patients (4.7%) developed IMN failure and simultaneous distant metastasis. The 5-year DFS, OS, and IMNFFS was 68.6%, 81.8%, and 95.3%, respectively. Non-triple-negative breast cancer, Ki-67≤10%, pathological complete response (CR) in tumor and axillary node, and radiologic CR of IMN after NAC were significant factors for predicting higher DFS; however, IMN radiation dose was not significant determinants for DFS. The 5-year DFS of patients with IMN-dose ≤ 50.0Gy and those with >50.0Gy was 86.7% and 76.7%, respectively (p=0.41).

A multimodality strategy including NAC, breast surgery, and IMN-covering radiotherapy was effective for patients with overt IMN+breast cancer. Even without an IMN dissection, most patients were IMN failure-free with an IMN-focusing radiotherapy.
A multimodality strategy including NAC, breast surgery, and IMN-covering radiotherapy was effective for patients with overt IMN + breast cancer. Even without an IMN dissection, most patients were IMN failure-free with an IMN-focusing radiotherapy.
The implementation of the proposal from the European Chemical Agency (ECHA) to restrict the use of nanoplastics (NP) and microplastics (MP) in consumer products will require reliable methods to perform size and mass-based concentration measurements. Analytical challenges arise at the nanometre to micrometre interface, e.g., 800nm-10µm, where techniques applicable at the nanometre scale reach their upper limit of applicability and approaches applicable at the micrometre scale must be pushed to their lower limits of detection.

Herein, we compared the performances of nine analytical techniques by measuring the particle size distribution and mass-based concentration of polystyrene mixtures containing both nano and microparticles, with the educational aim to underline applicability and limitations of each technique.

Light scattering-based measurements do not have the resolution to distinguish multiple populations in polydisperse samples. Nanoparticle tracking analysis (NTA), nano-flowcytometry (nFCM) and asyto accurately detect particles below 200 nm, and similarly to transmission electron microscopy (TEM) and flow cytometry (FCM), is not suitable for accurate mass-based concentration measurements. Alternatives for high-resolution sizing and concentration measurements in the size range between 60 nm and 5 µm are tunable resistive pulse sensing (TRPS) and centrifugal liquid sedimentation (CLS), that can bridge the gap between the nanometre and micrometre range.The absorption layer is a crucial factor for high-performance perovskite solar cells. In this work, the influence of the two components, methylammonium iodide (MAI) and formamidinium iodide (FAI) on the morphology, optical absorption and photovoltaic performances was systematically investigated. The results revealed that the surface morphologies of MAI/FAI based perovskite films were rougher, and the grain sizes became larger with increasing the FAI concentration. UV-Vis and photoluminescence spectra showed that there was a red shift with enhancing the FAI concentration. By the effective doping of FAI into the pristine MAI based perovskite film, the formation of a δ-FAPbI3 was successfully inhibited. As a result, the power conversion efficiency (PCE) of the perovskite solar cells based on mixed absorption layers was improved by about 27% compared to the pristine MAI based perovskite device.Adjusting the surface structures and electronic structures of metal nanocrystals (NCs) by the metal-organic interface interaction is an emerging strategy to enhance their electrocatalytic behavior. In this work, the d-phenylalanine-functionalized graphene (DPHE-GS) anchoring Pd NCs (denoted as Pd/DPHE-GS) was fabricated via the diazo-reaction followed by a simple chemical reduction. Owing to the metal-organic interface interaction between Pd NCs and DPHE, the size, distribution and electronic structures of Pd NCs on the surface of DPHE-GS can be adjusted. Therefore, the Pd/DPHE-GS shows the highest electrocatalytic activity and the most robust long-term durability and stability towards methanol and ethanol oxidation reaction (MOR and EOR) compared to the commercial Pd/C and other counterparts. This work presents an effective interface engineering strategy to enhance electrocatalytic property.Transition metal dichalcogenides (TMDs) have emerged as a promising material in the energy field due to their unique structural arrangement. In this work, ordered flower-like WSe2 nanosheet was synthesized through simple one-step hydrothermal method, and its cathode application for rechargeable Mg-ion batteries was assessed. The WSe2 cathode exhibits a high reversible capacity above 265 mAh g-1 at 50 mA g-1, excellent cycling life of 90% initial capacitance that can be ceaselessly harvested for 100 cycles at 50 mA g-1, and superior rate capability of 70% initial capacitance maintained even at the current density of 500 mA g-1. This work paves the way for the application of WSe2 cathode in Mg-ion and other rechargeable batteries.
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