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Protective Effect of Jiang Tang Xiao Ke Granules in opposition to Bone Muscle tissue IR by way of Initial of the AMPK/SIRT1/PGC-1α Signaling Path.
Finally, we studied the conservation of the Arabidopsis thaliana regulatory miR159-MYB module in apple in the context of the plant hormone abscisic acid homeostasis.Dehydration proteins (dehydrins, DHNs) confer tolerance to water-stress deficit in plants. We performed a comparative genomics and evolutionary study of DHN genes in four model Brachypodium grass species. Due to limited knowledge on dehydrin expression under water deprivation stress in Brachypodium, we also performed a drought-induced gene expression analysis in 32 ecotypes of the genus' flagship species B. distachyon showing different hydric requirements. Genomic sequence analysis detected 10 types of dehydrin genes (Bdhn) across the Brachypodium species. Domain and conserved motif contents of peptides encoded by Bdhn genes revealed eight protein architectures. Bdhn genes were spread across several chromosomes. Selection analysis indicated that all the Bdhn genes were constrained by purifying selection. Three upstream cis-regulatory motifs (BES1, MYB124, ZAT) were detected in several Bdhn genes. Gene expression analysis demonstrated that only four Bdhn1-Bdhn2, Bdhn3, and Bdhn7 genes, orthologs of wheat, barley, rice, sorghum, and maize genes, were expressed in mature leaves of B. distachyon and that all of them were more highly expressed in plants under drought conditions. Brachypodium dehydrin expression was significantly correlated with drought-response phenotypic traits (plant biomass, leaf carbon and proline contents and water use efficiency increases, and leaf water and nitrogen content decreases) being more pronounced in drought-tolerant ecotypes. Our results indicate that dehydrin type and regulation could be a key factor determining the acquisition of water-stress tolerance in grasses.It is important to consider the nutritional demand among genotypes of the same species to achieve high yields. Thus, the objective of this study was to determine the concentration of nutrients in leaves, flowers, grains, and fruit straw in conilon coffee genotypes. The experiment was carried out under a randomized block design, with three replications and the evaluation of nine genotypes. Leaf collections were carried out every three months, from August 2019 to May 2020. Flowers were collected at flowering in July 2019 and fruits in June 2020, upon complete maturation of the genotypes. The materials were dried in an oven and sent for laboratory analysis to determine the nutritional content. Data were subjected to analysis of variance followed by a comparison of means and estimates of genetic parameters and clustering using the hierarchical method (UPGMA). The nutrients found in the highest concentrations in the evaluated plant organs were N and K for macronutrients and Fe for micronutrients. For the leaves, the concentrations of the main nutrients were high in the first and reduced in the last evaluated periods, possibly due to mobilization to the fruits. Considering all the plant tissues evaluated, the order of concentration of macronutrients and micronutrients was N > K > Ca > P = Mg = S and Fe > B > Mn > Cu > Zn, respectively. For a nutritional diagnosis, it is important to take comparisons of the genetic diversity and evaluation periods into consideration.Wounding stress is an effective strategy to induce glucosinolate (GS) biosynthesis in broccoli. this website However, there is insufficient knowledge on the physiological and molecular mechanisms underlying this stress response. Herein, a chemical-genetic approach was applied to elucidate the role of jasmonic acid (JA), ethylene (ET), and reactive oxygen species (ROS) on the wound-induced biosynthesis of GS. Broccoli was processed into chops to induce wounding stress. Broccoli chops were treated with phenidone (PHEN) and diphenyleneiodonium chloride (DPI) as inhibitors of JA and ROS biosynthesis, respectively, whereas 1-methylcyclopropene (1-MCP) was applied as an inhibitor of ET action. Wounding stress induced the expression of genes related to the biosynthesis of indolic and aliphatic GS, which was correlated with the accumulation of GS and modulated by the inhibitors of signaling molecules applied. Results of gene expression analysis indicated that JA played a key role in the activation of most genes, followed by ROS. Furthermore, except for the CYP79B2 gene, PHEN and 1-MCP synergistically downregulated the expression of GS biosynthetic genes evaluated, showing that the interaction between JA and ET was fundamental to modulate GS biosynthesis. Results presented herein increased our knowledge of the physiological and molecular mechanisms governing the wound-induced biosynthesis of GS in broccoli.Birch tree bark-derived betulin has attracted scientific interest already for several centuries, being one of the first natural products identified from plants. However, the cellular events regulated by betulin and precise molecular mechanisms under these processes have been begun to be understood only recently. Today, we know that betulin can exert important anticancer activities through modulation of diverse cellular pathways. In this review article, betulin-regulated molecular signaling is unraveled and presented with a special focus on its participation in anti-inflammatory processes, especially by modulating nuclear factor-κB (NF-κB), prostaglandin/COX, and nuclear factor erythroid2-related factor 2 (Nrf2)-mediated cascades. By regulating these diverse pathways, betulin can not only affect the development and progression of different cancers, but also enhance the antitumor action of traditional therapeutic modalities. It is expected that by overcoming the low bioavailability of betulin by encapsulating it into nanocarriers, this promising natural compound may provide novel possibilities for targeting inflammation-related cancers.Tartary buckwheat bran, a byproduct of buckwheat milling, is commonly treated as waste. The present study examined the rutin content during successive infusions of roasted Tartary buckwheat bran and grain to develop a functional Tartary buckwheat tea. Samples (6 g) of roasted Tartary buckwheat bran and grain were rinsed with 300 mL of hot water (>95 °C) for 0.5 min. For the first infusion test, the tea infusion sample of roasted Tartary buckwheat bran contained a distinctly higher amount of rutin (389 mg/L) than that of the roasted Tartary buckwheat grain (68 mg/L). Overall, rutin was more effectively extracted from roasted Tartary buckwheat bran, as compared to roasted Tartary buckwheat grain.Sewage sludge improves agricultural soil and plant growth, but there are risks associated with its use, including high heavy metal content. In this study, experiments were carried out to investigate the role of endophytic Talaromyces pinophilus MW695526 on the growth of Triticum aestivum cultivated in soil amended with sewage sludge and its phytoremediation ability. T. pinophilus could produce gibberellic acid (GA) and stimulate T. aestivum to accumulate GA. The results showed that inoculation with T. pinophilus boosted plant growth criteria, photosynthetic pigments, osmolytes (soluble proteins, soluble sugars and total amino acids), enzymatic antioxidants (catalase, superoxide dismutase and peroxidase), K, Ca and Mg. On the other hand, it reduced Na, Na/K ratio, Cd, Ni, Cu and Zn in the growth media as well as in the shoot and root of T. aestivum. The results suggest that endophytic T. pinophilus can work as a barrier to reduce the absorption of heavy metals in T. aestivum cultivated in soil amended with sewage sludge.The anti-inflammatory and antihyperglycemic effects of polysaccharides extracted from Alhagimaurorum Medik. seeds, spontaneous shrub collected in Southern of Algerian Sahara were investigated. Their water extraction followed by alcoholic precipitation was conducted to obtain two water-soluble polysaccharides extracts (WSPAM1 and WSPAM2). They were characterized using Fourier transform infrared, 1H/13C Nuclear Magnetic Resonance, Gas Chromatography-Mass Spectrometry and Size Exclusion Chromatography coupled with Multi-Angle Light Scattering. The capacity of those fractions to inhibit α-amylase activity and thermally induced Bovine Serum Albumin denaturation were also investigated. WSPAM1 and WSPAM2 were galactomannans with a mannose/galactose ratio of 2.2 and 2.4, respectively. The SEC-MALLS analysis revealed that WSPAM1 had a molecular weight of 1.4 × 106 Da. The investigations highlighted antinflammatory and antihyperglycemic effects in a dose-dependant manner of WSPAM1 and WSPAM2.A systematic approach to quantifying the weed-crop balance through the flux of solar radiation was developed and tested on commercial fields in a long-established Atlantic zone cropland. Measuring and modelling solar energy flux in crop stands has become standard practice in analysis and comparison of crop growth and yield across regions, species and years. In a similar manner, the partitioning of incoming radiation between crops and the in-field plant community may provide 'common currencies' through which to quantify positive and negative effects of weeds in relation to global change. Here, possibilities were explored for converting simple ground-cover measures in commercial fields of winter and spring oilseed rape in eastern Scotland, UK to metrics of solar flux. Solar radiation intercepted by the crops ranged with season and sowing delay from 129 to 1975 MJ m-2 (15-fold). Radiation transmitted through the crop, together with local weed management, resulted in a 70-fold range of weed intercepted radiation (14.2 to 963 MJ m-2), which in turn explained 93% of the corresponding between-site variation in weed dry mass (6.36 to 459 g m-2). Transmitted radiation explained almost 90% of the variation in number of weed species per field (12 to 40). The conversion of intercepted radiation to weed dry matter was far less variable at a mean of 0.74 g MJ-1 at both winter and spring sites. The primary cause of variation was an interaction between the temperature at sowing and the annual wave of incoming solar radiation. The high degree of explanatory power in solar flux indicates its potential use as an initial predictor and subsequent monitoring tool in the face of future change in climate and cropping intensity.The genus Utricularia includes around 250 species of carnivorous plants, commonly known as bladderworts. The generic name Utricularia was coined by Carolus Linnaeus in reference to the carnivorous organs (Utriculus in Latin) present in all species of the genus. Since the formal proposition by Linnaeus, many species of Utricularia were described, but only scarce information about the biology for most species is known. All Utricularia species are herbs with vegetative organs that do not follow traditional models of morphological classification. Since the formal description of Utricularia in the 18th century, the trap function has intrigued naturalists. Historically, the traps were regarded as floating organs, a common hypothesis that was maintained by different botanists. However, Charles Darwin was most likely the first naturalist to refute this idea, since even with the removal of all traps, the plants continued to float. More recently, due mainly to methodological advances, detailed studies on the trap function and mechanisms could be investigated.
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