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Soil enzyme activities including dehydrogenase, urease, and catalase increased up to 5-fold. Soil bacterial diversity and structure were changed, being largely composed of Proteobacteria, Actinobacteria, Patescibacteria, Bacteroidetes, and Firmicutes. These findings provide a green and sustainable approach to decontaminating complex-polluted environments with comprehensive improvement of soil health.The nanoparticles of zeolitic imidazolate framework (ZIF-67) were synthesized and added to ethanolamine/deep eutectic solvent solution to form nanofluid system. The dynamic removal performance of prepared nanofluid system for hydrogen sulfide was investigated. For the system based on choline chloride and urea, the introduction of nanoparticles showed significant enhancement effect on the desulfurization performance. The optimal mass fraction of nanoparticles in nanofluid systems were identified as 0.1%. Besides, the experimental results showed that the prepared nanofluid systems have high regeneration performance, and the presence of moderate moisture is beneficial to the regeneration process. The absorbents and nanoparticles before and after absorption were characterized by Fourier transform infrared spectra, nuclear magnetic resonance, scanning electron microscope, energy dispersive spectrum, X-ray diffraction and X-ray photoelectron spectroscopy. The characterization results showed that the surface of nanoparticle was covered by CoS2 after absorption.Heavy metal contamination has caused serious threats to surrounding fragile environments and human health. While the novel microbial-induced carbonate precipitation (MICP) technology in the recent years has been proven effective in improving material mechanical and durability properties, the mechanisms remedying heavy metal contamination still remain unclear. In this study, the potential of applying the MICP technology to the lead remediation under the effects of urease activity and calcium source was explored. The values of OD600 corresponding to the ureolytic bacterial activity, electrical conductivity (EC), urease activity (UA) and pH were applied to monitor the degree of urea hydrolysis. Further, the carbonate precipitations that possess different speciations and cannot be distinguished through test tube experiments were reproduced using the Visual MINTEQ software package towards verifying the validity of the proposed simulations, and revealing the mechanisms affecting the lead remediation efficiency. The findings summarised in this work give deep insights into lead-contaminated site remediation engineering.Biological treatment is an efficient and economical process to remove thiamphenicol (TAP) residues from the environment. The discovery of TAP-degrading bacteria and the decryption of its biodegradation mechanism will be beneficial to enhance the biological removal of TAP. In this study, Sphingomonas sp. CL5.1 was found to be capable of catabolizing TAP as the sole carbon, nitrogen, and energy source. This strain could degrade 93.9% of 25 mg/L TAP in 36 h, and remove about 11.9% of the total organic carbon of TAP. A novel metabolism pathway of TAP was constructed, and the enzymes involved in TAP metabolism in strain CL5.1 were predicted via proteomic and metabolic analysis. TAP was proposed to be transformed to O-TAP via oxidation of C3-OH and DD-TAP via dehydration of C3-OH and dehydrogenation of C1-OH. A novel glucose-methanol-choline (GMC) family oxidoreductase CapO was predicted to be involved in the oxidation of C3-OH. O-TAP was supposed to be further cleaved into DCA, glycine, and PMB. Glycine might be a pivotal direct nitrogen source for strain CL5.1, and it could be involved in nitrogen metabolism through the glycine cleavage system or directly participate in the biosynthetic processes.Nylon has been widely used all over the world, and most of it eventually enters the aquatic environment in the form of microplastics (MPs). However, the impact of Nylon MPs on aquatic ecosystem remains largely unknown. Thus, the long-term biological effects and toxicity mechanism of Nylon MPs on Microcystis aeruginosa (M. aeruginosa) were explored in this study. Ipatasertib Results demonstrated that Nylon MPs had a dose-dependent growth inhibition of M. aeruginosa at the initial stage, and the maximum inhibition rate reached to 47.62% at the concentration of 100 mg/L. Meanwhile, Nylon MPs could obstruct photosynthesis electron transfer, reduce phycobiliproteins synthesis, destroy algal cell membrane, enhance the release of extracellular polymeric substances, and induce oxidative stress. Furthermore, transcriptomic analysis indicated that Nylon MPs dysregulated the expression of genes involved in tricarboxylic acid cycle, photosynthesis, photosynthesis-antenna proteins, oxidative phosphorylation, carbon fixation in photosynthetic organisms, and porphyrin and chlorophyll metabolism. According to the results of transcriptomic and biochemical analysis, the growth inhibition of M. aeruginosa is inferred to be regulated by three pathways photosynthesis, oxidative stress, and energy metabolism. Our findings provide new insights into the toxicity mechanism of Nylon MPs on freshwater microalgae and valuable data for risk assessment of MPs.By exploiting the superior adsorption capacity of ultra-stable Y-type zeolite (USY) and accurate input of energy by electromagnetic induction field (EMIF) technique, we successfully designed a highly energy-efficient system to eliminate gaseous toluene a common air pollutant. Pristine USY as adsorbent enriches gaseous toluene by a factor of fifteen, via room-temperature adsorption and then EMIF-driven thermal desorption. This operation model involving intermittent heating and mass transfer saves a lot of energy. Especially during temperature rising, 98.9% electric energy can be saved by the EMIF heating in comparison with conventional furnace approaches. In the bi-functional "adsorption-catalytic oxidation" 1Pt/USY, the concentrated toluene undergoes direct oxidation into CO2 rather than desorption when the EMIF heating starts, so one-step enrichment and mineralization are realized. In addition, the developed bi-functional system operates between adsorption and catalytic decomposition flexibly, which makes it ideal for cleaning VOCs emitted from intermittent sources.Applications of nanocellulose as a water treatment material are being actively pursued based on its interesting properties, such as renewability, large specific surface area, hydrophilic surface chemistry, and biodegradability. This study used carboxymethyl cellulose nanofibrils (CMCNFs) to prepare a typical bead-type adsorbent with improved structural stability as an actual water treatment restoration material. In addition, a cationized nanocellulose adsorbent was prepared by introducing polyethyleneimine (PEI) on the surface of the CMCNF (P/CMCNF), the removal efficiency of Cr(VI) was evaluated, and its mechanism was elucidated. As a result, the P/CMCNF beads showed an excellent Cr(VI) removal capacity of 1302.3 mg/g, the best result among cellulose-based adsorption materials. Cr(VI) was effectively removed by electrostatic attractions combined with chemical reduction and chelation mechanisms. Furthermore, the macrobead fabrication and PEI surface modification process improved the underwater stability of the P/CMCNF, and it showed excellent reuse efficiency.Azodicarbonamide (ADA) in flour can be easily decomposed to semi-carbazide and biuret, exhibiting strong genotoxicity in vitro and carcinogenicity. Glutathione (GSH) can be conjugated with some ketone-containing compounds and unsaturated aldehydes to form toxic metabolites. Here, a novel ratio fluorescence probe based on blue emitting biomass-derived carbon dots (BCDs) and yellow emitting 2,3-diaminophenazine (OxOPD) was prepared for the bifunctional determination of glutathione (GSH) and ADA. This strategy includes three processes (1) Ag+ oxidizes o-phenylenediamine (OPD) to produce OxOPD. The peak at 562 nm was enhanced, and the peak at 442 nm was reduced due to fluorescence resonance energy transfer (FRET), (2) glutathione binds Ag+ and inhibits the production of OxOPD, (3) ADA oxidizes GSH to form GSSG, resulting in the release of Ag+ by GSH. Therefore, the newly designed ratio fluorescence probe can be based on the intensity ratio (I442/I562) changes and significant fluorescent color changes to detect GSH and ADA. Moreover, a smartphone WeChat applet and a yolov3-assisted deep learning classification model have been developed to quickly detect GSH and ADA on-site based on an image processing algorithm. These results indicate that smartphone ratiometric fluorescence sensing combined with machine learning has broad prospects for biomedical analysis.Excessive heavy metals (HMs) in livestock manure due to additives over-use limits its recycling through composting. However, few studies have investigated the interactive influence of microbial communities, functional genes, and environmental factors in HM immobilization during composting. Therefore, treatments with different C/N ratios (151, 201, 251) and bulking agents (maize straw, green waste, vinasse) were conducted to explore the HMs immobilization pathways using structural equation model (SEM). Results confirmed the promoting effect of C/N ratio of 201 and vinasse on microbial diversity, thus leading to greater HMs immobilization rate. Meanwhile, the dominant microbial phyla of Cu/Zn, Cd, As, and Cr were identified as Proteobacteria, Firmicutes, Chytridiomycota, and Bacteroidota, respectively. Moreover, the significant correlation between functional genes (copC, mt, cbh1, aoxB, yieF) and HMs illustrated potential immobilization effects of metal-binding proteins on Cu and Zn, humus complexation on Zn, and oxidase/reductase on As/Cr. Finally, SEM indicated that the redistribution of Cu, Zn, As, and Cr fractions was interactively influenced by environmental factors (organic matter, pH, electrical conductivity, and total phosphorus), microbial communities, and functional genes, while Cd was directly regulated by organic matter and total phosphorus. These results may provide a deeper understanding of HM immobilization pathways during swine manure composting.Few studies have investigated preferred driving posture involving real-world driving. For this purpose, seven preferred joint angles of 127 participants were recorded three times from sagittal plane photographs after a short, standardized drive on public roads. Participants' individual driving posture preferences were determined using a word-based questionnaire. The inter- and intraindividual joint angle differences were calculated and some significant effects of stature, age, and gender on these joint angle differences were found. Additionally, significant correlations were found between posture preferences and joint angles, except for the lower extremities. The results show that the factors stature, age, gender, and posture preferences have influence on the preferred joint angles. However, other human-, vehicle-, and context-related factors also seem relevant and should be considered in future studies. The results provide important field-based knowledge for an application-oriented understanding of driving posture and for the human-centered design process of future vehicle interior research and development.
Homepage: https://www.selleckchem.com/products/gdc-0068.html
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