Notes

Differential unsafe effects of jasmonate responses within several jaz mutants.
The gall midge Gephyraulus lycantha (Diptera Cecidomyiidae) is a serious gall-forming pest that causes devastating damage in the wolfberry, Lycium barbarum (Solanaceae) in Northwest China. In the present study, the external morphology and ultrastructure of the antennae and the antennal sensilla of G. lycantha were examined by scanning electron microscopy. The results show that the moniliform antenna of G. lycantha consisted of a scape, pedicel, and flagellum, and exhibited obvious sexual dimorphism. The male antennae were significantly longer than those of females. Moreover, male flagellomeres were spheroidal nodes separated by slender internodes, whereas those of females were cylindrical with no obvious internodes. There were sex and individual differences in antennal segment number. Male antennae had 10 - 16 flagellomeres, most of which had 15, while female antennae consisted of 8 - 14 flagellomeres, most of which had 12. Moreover, a pair of antennae in the same individual had different numbers of flagellomed on their morphology and ultrastructure. These results provide an important basis for further research on chemical communication and strategies for the control of G. lycantha, and it will be able to serve future group Taxonomy studies (species of cecidomyiidae), providing new taxonomic characters (general ultrastructural morphology, number of sensilla and antennal segments, distribution of different types of setae, types and subtypes sensilla), which varies between species and subspecies.This paper proposes a method of designing a magnetic lens circuit based on axial transform theory. A unified mathematical description of the magnetic circuit profile is given within a medial axial transform in differential geometry. The objective constraint and geometric constraint equations of the magnetic circuit form a closed set of differential equations. Lastly, the optimized magnetic circuit is obtained using the solutions of the equations. The optical performance of the magnetic lens is significantly improved.Mucosal-associated invariant T (MAIT) cells are subsets of T cells abundant in human mucosal tissues and in blood. These cells are activated directly by cytokines or by vitamin B metabolites antigen presentation. MAIT cells possess antimicrobial potential against viruses and bacteria through production of cytokines and cytotoxic molecules. MAIT cells generally reduce in numbers and function during viral and bacterial infections/diseases. Mice and humans lacking MAIT cells cannot effectively control bacterial infections. MAIT cells respond rapidly to infections and are rapidly recruited to the site of vaccination or infection including the lungs where they can be involved in controlling local inflammation. These characteristics of MAIT cells offer them a unique potential to be explored as potential targets for vaccines.
Patients with cirrhosis are susceptible to bacterial infections (BIs) that are major causes of specific complications and mortality. However, the diagnosis of BIs can often be difficult in advanced disease stage since their symptoms may overlap with the ones of acute decompensation (AD). Soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) is released from monocytes/macrophages and neutrophils during activation and has been reported to correlate with activity of various inflammatory processes. We investigated its diagnostic and prognostic performance in patients with cirrhosis and BI.

Sera of 269 patients were assayed for sTREM-1 by ELISA (172 outpatients and 97 patients with AD of whom 56 had BI). We investigated capacity of sTREM-1 to identify patients with BI and conducted a 90-day follow-up observational study to assess its possible association with short-term mortality.

sTREM-1 levels were significantly higher in patients with more severe liver disease, BI, and acute-on-chronic liver failure than in patients without these conditions. sTREM-1 had similar accuracy to CRP identifying BI [sTREM-1 AUROC (95%CI) 0.804 (0.711-0.897), p < 0.0001; CRP 0.791 (0.702-0.881), p < 0.0001)] among AD patients. The combination of these two molecules and the presence of ascites into a composite score significantly increased their discriminative power (AUROC 0.878, 95%CI 0.812-0.944, p < 0.0001). High sTREM-1 level (>660 pg/mL) was an independent predictor of 90-day mortality in patients with BI [HR 2.941, (95%CI 1.009-8.573), p = 0.048] in our multivariate model.

Use of sTREM-1 could increase the recognition of BIs in cirrhosis and help clinicians in mortality risk assessment of these patients.
Use of sTREM-1 could increase the recognition of BIs in cirrhosis and help clinicians in mortality risk assessment of these patients.Yttria coated graphite crucibles are widely used to handle molten refractory and radioactive metals like uranium and plutonium. However, the coated layer suffers damages like cracking and peeling off owing to thermal cycles. As a result, removal of the yttria layer from the graphite surface is essential to ensure reuse of the crucible and minimization of radioactive waste. The present work investigates intensified dissolution of yttria from the coated graphite samples using ultrasound as a non-destructive decontamination technique to recycle the graphite substrate. The optimum conditions established for maximum dissolution were 8 M as acid strength, frequency of 30 kHz, temperature of 45 °C and power density of 8 W cm-2 that resulted in maximum dissolution of 52% in 30 min. Use of an oxidant H2O2 to the acid, did not yield any improvement in the dissolution kinetics, instead, increased oxidation of the graphite substrate was observed, leading to the anomalous weight gain of the graphite substrate despite surface erosion. Effect of ultrasound on the dissolution was pronounced, with almost a threefold increase compared to dissolution performed under silent conditions. Rates of dissolution of yttria from the substrate of different densities and pore size distribution were also studied. The dissolution was slowest from graphite of density 1.82 g cm-3 as the pore size distribution was conducive to accommodate the yttria particles. The dissolution in nitric acid followed ash layer diffusion controlled kinetics. The study has demonstrated the efficacy of application of ultrasound for accelerated decontamination of graphite substrates.Effects of ultrasound (US, 300, 400, and 500 W) and slightly acidic electrolyzed water (SAEW, 10, 30, and 50 mg/L) combination on inactivating Rhizopus stolonifer in sweet potato tuberous roots (TRs) were investigated. US at 300, 400, and 500 W simultaneous SAEW with available chlorine concentration of 50 mg/L at 40 and 55 °C for 10 min significantly inhibited colony diameters (from 90.00 to 6.00-71.62 mm) and spores germination (p less then 0.05). US + SAEW treatment could destroy cell membrane integrity and lead to the leakage of nucleic acids and proteins (p less then 0.05). Scanning and transmission electron microscopy results showed that US + SAEW treatment could damage ultrastructure of R. stolonifer, resulted in severe cell-wall pitting, completely disrupted into debris, apparent separation of plasma wall, massive vacuoles space, and indistinct intracellular organelles. SB273005 US500 + SAEW50 treatment at 40 and 55 °C increased cell membrane permeability, and decreased mitochondrial membrane potential of R. stolonifer. In addition, US500 + SAEW50 at 40 °C and US300 + SAEW50 at 55 °C controlled R. stolonifer growth in sweet potato TRs during 20 days of storage, suggesting effective inhibition on the infection of R. stolonifer. Therefore, US + SAEW treatment could be a new efficient alternative method for storing and preserving sweet potato TRs.Combining plasmonic and photonic elements, optoplasmonic hybrid structure exhibits excellent optical properties beyond conventional plasmonic or photonic structures. In this work, the optoplasmonic film consists of SiO2 microsphere and Au film without any nanostructures is investigated. With the help of a microsphere, the intensity of surface enhanced Raman spectroscopy (SERS) on Au film is highly enhanced (~1000 times) compared to bare Au film. The simulated electromagnetic field points out the enhancement caused by the optical lens effect of SiO2 microsphere that high light intensity is generated under the microsphere to excite surface plasmon on Au film. Furthermore, our data demonstrates the microsphere lens enhancement is greatly influenced by the size of the SiO2 microsphere and wavelength of incident light. This interesting film with a simple configuration could overcome the challenges in the fabrication and store process induced by nanostructures, which play an important role in SERS application. Our work not only enlarges the knowledge of the optoplasmonic hybrid structure, but also exhibits excellent application prospective in light harvest field e.g. enhanced spectrum, photocatalysis, optothermal effect, and hot electron generation, etc.Pulsed terahertz (THz) broadband spectroscopy and imaging were applied to investigate ex vivo rabbit corneal scar tissues. Scars with different depths and densities were created by laser ablation on four corneal samples while two corneal samples untouched were used as control. The THz time-domain spectroscopy and reconstructed images illustrated the scar tissue density and gave out depth variation distribution profiles. Calculated absorption coefficient spectra displayed extra absorption peaks compared with control corneas and refractive index spectra showed the optical properties changed at the corneal scar locations. THz spectroscopy and imaging demonstrated adequate contrast in reviewing the scar densities and locations and showed potential applications in depicting spatial distribution and composition changes of corneal scars.The excessive use or abuse of antibiotics on dairy cows leads to residues in milk, which can represent a public health risk. However, in recent years the β-Lactamase was illegally used to degrade residual antibiotics in milk, which makes the traditional antibiotic detection methods ineffective. Therefore, there is an extremely urgent need for multi-analyte analysis techniques for the detection of antibiotic residues. Herein, we reported an ultra-fast, facile, and sensitive solid-phase microextraction (SPME)-surface enhanced Raman scattering (SERS) platform for the detection of degraded antibiotics-2-mercapto-5-methyl-1,3,4-thiadiazole (MMT). The results showed that the log-log plot of SERS intensity to MMT concentration exhibits a superior linear relationship (R2 = 0.992) in the concentration range of 0.5-1000 μM, with a detection limit of 0.11 μM. The silver nanoparticle-decorated TiO2 nanotube array was successfully used as an all-in-one SPME-SERS substrate in the extraction and identification of the antibiotic degradation products in real milk. Due to the rapid pre-treatment, good reproducibility, and self-cleaning, the proposed SPME-SERS method has a great promise to be applied as a powerful tool for on-site detection in the field of food safety.We present the first complete dispersion analysis of a sucrose single crystal in the infrared spectral region between 4000 and 400 cm-1 by means of an adapted generalized dispersion analysis employing the naturally grown crystal faces. The gained dielectric tensor function and the oscillator parameters were confirmed by forward calculation of reflection spectra of different orientations. Reliable growth of large-sized sucrose crystals makes it candidates for doping with photonically active materials.
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