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Self-consciousness of keratinocyte ferroptosis depresses psoriatic infection.
Microbial contamination and damaging effects due to environmental factors (temperature, humidity) of old books is an extremely important issue in the heritage preserving field, being a great threat to this unique cultural and literary treasure. This paper analysed the indoor environmental conditions, the microbiological characteristics of the air and the condition itself of 27 clerical books (from eighteenth to nineteenth centuries), randomly chosen from the library of Bistrița Monastery, Romania. The analysis revealed that most of the books were contaminated and damaged by moulds and yeasts. The statistical analysis (chi-square test) shows that the incidence of mould species was not coincidental. The most common isolated fungi discovered on old books were Penicillium spp. (40.7%), Cladosporium spp. (33.3%) and Fusarium spp. (29.6%). Fungal contamination was observed in 17 books. Microbiota air tests have shown possible contamination by air flow. Often, the measured temperature/humidity values are not in line with the requirements of the legislation in force, especially during the cold seasons, the average minimum temperature varying from 3 to 6 °C to an average maximum temperature of 22-25 °C; the values of relative humidity were between 27 and 77%. In terms of storage conditions, the recorded values of temperature and humidity are extremely harmful to the stored documents. Therefore, these indoor environmental conditions have to be improved, in order to reduce/eliminate potential sources of fungal contamination and to achieve optimal environmental preserving conditions.Previous studies consider ICT a two-edged sword that can harm or benefit the environment. In recent years, ICT penetration has considerably increased in the ASEAN-6 countries and the leaders of ASEAN are willing to bring a digital revolution by increasing ICT infrastructure and reducing trade barriers in the region under the Master Plan of ASEAN Connectivity-2025. Hence, this paper explores the effect of ICT and the recently developed trade globalization index on CO2 emissions in ASEAN-6 countries. The study relies on advanced panel econometric approaches, including Westerlund (2007, 2008) and Pedroni cointegration tests, CUP-FM long-run method, and panel DH causality approach. The results suggest cointegration among variables. The results of CUP-FM indicate that ICT contributes to improving environmental quality by mitigating CO2 emissions. Similarly, trade globalization is also sustainable in the region as it reduces emissions. The results are also confirmed by using the CUP-BC estimator. The findings from the DH causality test unfold causality from ICT and trade globalization index to CO2 emissions. Besides, the long-run estimates reveal the detrimental effect of energy consumption on emissions and the U-shaped association between GDP and emissions. Moreover, unidirectional causality from ICT to trade globalization index and energy consumption indicates that ICT influences trade globalization and energy consumption. Finally, environmental policies in the context of ASEAN are extensively discussed.CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in a multi-well cell culture plate to profile RNA polymerase II. Long (> 270 bp) DNA fragments released by CUT&RUN corresponded to the bimodal peak around the transcription start sites, as previously seen with chromatin immunoprecipitation. However, we found that short ( less then  120 bp) fragments identify a well-defined peak localised at the transcription start sites. This distinct DNA footprint of short fragments, which constituted only about 5% of the total reads, suggests the transient positioning of RNA polymerase II before promoter-proximal pausing, which has not been detected in the physiological settings by standard chromatin immunoprecipitation. We showed that the positioning of the large-size-class DNA footprints around the short-fragment peak was associated with the directionality of transcription, demonstrating the biological significance of distinct CUT&RUN footprints of RNA polymerase II.A physiologically based pharmacokinetic (PBPK) human model for alpelisib, an oral α-specific class I phosphatidylinositol-3-kinase (PI3K) inhibitor, was established to simulate oral absorption and plasma pharmacokinetics of healthy subjects to allow model-informed drug development. The GastroPlus™ model consisted of an advanced absorption gut model, which was linked to a 2-compartmental model. Systemic clearance and volume of distribution were estimated using population pharmacokinetics (popPK). Various food effect and pH-mediated absorption drug-drug interaction (DDI) scenarios were modeled. In fasted healthy subjects, simulated absorption was lower (ca. 70% for a 300-mg dose) due to pH and bile acid concentration-dependent solubility. Ranitidine showed a significant pH-mediated DDI effect only in the fasted but not fed state. The PBPK model identified that more drug is absorbed in the fed state, and alpelisib intestinal permeability is rate limiting to systemic exposure. Simulations for healthy subject showed a positive food effect with ca. 2-fold increase in plasma Cmax and 1.5-fold increase in AUC0-inf with a meal compared with fasted conditions. The PBPK model was verified using clinical food effect data with pivotal clinical formulation (PCF) and then applied to predict the performance of a commercial formulation (CF) in healthy volunteers. The model successfully predicted the outcome of a clinical bioequivalence study for PCF and CF with included in vitro dissolution data, both fasted and fed state. Estimated predictive errors (based on plasma Cmax, AUC0-t) were equal or below 30%. The alpelisib model for healthy subjects enables future bioequivalence formulation assessments, in fasted, fed, or altered pH conditions. Graphical Abstract.
Epilepsy is a global public health problem that causes a profound physical, psychological and social consequences. However, as such evidence in our country is limited, this study aimed to assess the health-related quality of life (HRQOL) and associated factors among patients with epilepsy.

An institution-based cross-sectional study was conducted on 370 patients with epilepsy. The Quality of Life in Epilepsy Inventory-31 (QOLIE-31) was used to measure HRQOL. Multiple linear regression was fitted to assess the association between HRQOL and the independent variables, and a P-value < 0.05 and a 95% confidence interval were used to declare statistical significance.

More than 55% of the participants were male, and the mean age of the participants was 29.64 (11.09) years. The overall HRQOL scorewas 55.81 (14.00). The scale scores ranged from 46.50 (15.55) to 64.98 (19.43). Out of the seven scales, the energy scale score was the lowest. Frequency of seizure, anxiety, depression, perceived stigma and adverse drug event were negatively associated with HRQOL, whereas social support had a significant positive association.

This study revealed that the HRQOL of patients was low and that its energy and emotional scales were the most affected. The presence of depression, anxiety and stigma adversely affected patient HRQOL. Therefore, healthcare professionals should be aware of the emotional state of the role it plays for HRQOL. Interventions aimed at reducing psychosocial problems and stigmatization are also needed to improve the patient HRQOL.
This study revealed that the HRQOL of patients was low and that its energy and emotional scales were the most affected. The presence of depression, anxiety and stigma adversely affected patient HRQOL. Therefore, healthcare professionals should be aware of the emotional state of the role it plays for HRQOL. Interventions aimed at reducing psychosocial problems and stigmatization are also needed to improve the patient HRQOL.
Few previous studies estimated the association between micronutrient status and sleep quality; no previous work was done in patients with depression compared with healthy controls.

Using a case-control design, 96 patients with depression were age- and sex-matched with 96 healthy controls. Dietary assessment utilized a standardized questionnaire, and analysis focused comprehensively on 18 micronutrient items. Sleep quality was measured using the Pittsburg Sleep Quality Index (PSQI). Descriptive statistics were used to summarize findings. Logistic regression analysis was used to identify predictors of poor sleep quality.

Patients with depression had a significantly lower sleep quality than controls with PSQI scores of 7.3 ± 2.7 and 5.1 ± 2.5, respectively. The prevalence of poor sleep quality in patients with depression was almost double the prevalence of poor sleep quality in the general population. The micronutrient status of vitamin B12 and Mg successfully predicted sleep quality in healthy controls. However, in patients with depression, micronutrient status failed to predict sleep quality.

The current research showed that sleep quality was positively associated with Mg intake, and negatively associated with vitamin B12 in healthy adults. For patients with depressionin this study sample, sleep quality was not associated with micronutrient intake status. Further research is needed to determine if intake of micronutrients can improve sleep and/or depressive symptoms in patients with depression.
The current research showed that sleep quality was positively associated with Mg intake, and negatively associated with vitamin B12 in healthy adults. For patients with depression in this study sample, sleep quality was not associated with micronutrient intake status. Further research is needed to determine if intake of micronutrients can improve sleep and/or depressive symptoms in patients with depression.One of the most complex health disproportions in the human body is the metabolic syndrome (MetS). Tinengotinib It can result in serious health consequences such as type 2 diabetes mellitus, atherosclerosis or insulin resistance. The center of energy regulation in human is AMP-activated protein kinase (AMPK), which modulates cells' metabolic pathways and protects them against negative effects of metabolic stress, e.g. reactive oxygen species. Moreover, recent studies show the relationship between the AMPK activity and the regulation of DNA damage repair such as base excision repair (BER) system, which is presented in relation to the influence of MetS on human genome. Hence, AMPK is studied not only in the field of counteracting MetS but also prevention of genetic alterations and cancer development. Through understanding AMPK pathways and its role in cells with damaged DNA it might be possible to improve cell's repair processes and develop new therapies. This review presents AMPK role in eukaryotic cells and focuses on the relationship between AMPK activity and the regulation of BER system through its main component-8-oxoguanine glycosylase (OGG1).Morphometric and molecular divergence among four butterfly species of the families Nymphalidae and Pieridae from the western Himalaya region were investigated using molecular tools, traditional morphometric measures and a truss network system. The considered species were Danaus chrysippus, Vanessa cardui, Pieris brassicae and Pieris canidia. Traditional taxonomy is sometimes unable to discriminate cryptic species or species that have close morphological features. Although taxonomists carefully examine external body features to differentiate the species; however, there is a risk for misidentification during a visual assessment of cryptic species. Therefore, we aimed to use the truss network system of 14 morphological landmarks interconnected to yield 90 variables about molecular taxonomy. Principal component analysis (PCA), discriminant function analysis (DFA) and cluster analysis (CA) were employed to determine morphometric variations. In the traditional analysis, 79, 68, 16 and 5 characters out of 90 were found significant (p  less then  0.
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