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The benefits of vascularization are clear avoidance of mass transfer limitation and oxygen deprivation, a significant decrease in cell necrosis, and consequently bone development, regeneration and remodeling. Here, we discuss specific techniques to avoid pitfalls and optimize vascularization results of tissue-engineered bone. Cell source, scaffold modifications and bioreactor design, and technique specifics all play a critical role in this new, and rapidly growing method for bone defect reconstruction. Given the crucial importance of long-term survival of vascular network in physiological function of 3D engineered-bone constructs, greater knowledge of vascularization approaches may lead to the development of new strategies towards stabilization of formed vascular structure.Traction force microscopy has been established as the accepted method for evaluating cell-induced mechanical stresses to their microenvironments, typically using two-dimensional (2D) elastic, synthetic gel-substrates. As cells naturally experience 3D environments in vivo, traction microscopy has been adapted to 3D gels; cells can be tracked over time in 3D. Microscopy images acquired in several fields-of-view e.g. in a time series, may experience drift, which can produce artefactual results that may appear valid and lead to flawed analysis. Hence, we have developed an algorithm for 2D/3D de-drifting of cell-images on 3D gels with fiducial markers (beads) as anchor points. Both lateral and vertical de-drifting are performed using gel-internalized beads, as those used in traction microscopy experiments; this eliminates need for immobilizing beads under the gel for de-drifting, and reduces experiment time. We introduce simulations of initially grid-ordered dots (beads) that are radially displaced to experimentally observed distances, while also applying additive drift. This facilitates testing and demonstration of the de-drifting procedures in 2D/3D. We demonstrate the importance of applying de-drifting using both computer-simulated drifts and experimentally observed drifts in confocal microscopy images. We show that our de-drifting algorithm can remove lateral and/or vertical drift revealing even small, underlying signals. The 2D/3D de-drifting algorithm, crucial for accurate identification of cell-induced marker-displacement, as well as the bead simulations, will shorten traction microscopy experiments and facilitate optimization of the experimental protocols.Toe walking is observed in pathological populations including cerebral palsy, stroke, and autism spectrum disorder. To understand pathological toe walking, previous studies have analyzed non-habitual toe walking. These studies found sagittal plane deviations between heel-toe and toe walking at the hip, knee, and ankle. Further investigation is merited as toe walking may involve altered biomechanics at more distal joints, such as the midtarsal joint. The purpose of this study was to examine biomechanical differences between rearfoot strike walking (RFSW) and non-rearfoot strike walking (NRFSW) in the midfoot and ankle. We hypothesized that during NRFSW, midtarsal kinematics would diverge from those during RFSW in all three cardinal planes and ankle kinematics would display increased supination. Twenty-four healthy females walked overground with both walking patterns. Motion capture, electromyography (EMG), and force plate data were collected. A validated multi-segment foot model was used with mean difference waveform analyses to compare walking conditions during stance. Significantly different kinematics were found in all three planes for the midtarsal and ankle joint during NRFSW. The NRFSW midtarsal joint exhibited increased plantarflexion, eversion, and adduction with the largest differences occurring at initial contact and in the sagittal plane. The NRFSW ankle exhibited increased supination at initial contact and during early stance. These findings indicate that toe walking alters both distal and proximal foot joint kinematics in multiple planes. This may further the understanding of altered biomechanics during toe walking while providing a basis for future analyses of pathological gait.This study investigates the effects of treadmill control algorithms on spatiotemporal variables when walking on a self-paced (SP) treadmill. click here Ten healthy subjects walked at their preferred walking speed for 15 min under three different treadmill control modes. Stride time, stride length, and stride speed were measured using an inertial measurement unit. The mean, coefficient of variance, Poincaré descriptors, and gait dynamics were calculated for each parameter. The mean values of stride length and stride speed were significantly increased when the treadmill had a quick response speed to the user's walking behavior. The long-term variability of stride length and stride speed was significantly affected by the treadmill control algorithms. A reduced strength of long-range correlations of stride time and stride speed was found when walking on the SP treadmill with suppressed treadmill accelerations and small velocity variations. We suggest that the suppression of treadmill acceleration provides more adaptability and less constraint to the user during SP treadmill walking. Although further research is required, the present work provides a basis for interpreting the influence of treadmill control algorithms on human gait.During phonation, human vocal fold tissues are subjected to combined tension, compression and shear loading modes from small to large finite strains. Their mechanical behaviour is however still not well understood. Herein, we complete the existing mechanical database of these soft tissues, by characterising, for the first time, the cyclic and finite strains behaviour of the lamina propria and vocalis layers under these loading modes. To minimise the inter or intra-individual variability, particular attention was paid to subject each tissue sample successively to the three loadings. A non-linear mechanical behaviour is observed for all loading modes a J-shape strain stiffening in longitudinal tension and transverse compression, albeit far less pronounced in shear, stress accommodation and stress hysteresis whatever the loading mode. In addition, recorded stress levels during longitudinal tension are much higher for the lamina propria than for the vocalis. Conversely, the responses of the lamina propria and the vocalis in transverse compression as well as transverse and longitudinal shears are of the same orders of magnitude.
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