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Effect of Dirt Qualities and Getting older Time upon Dental along with Inhalation Bioaccessibility regarding Copper Oxide Nanoparticles inside Garden soil.
Pink stem borer, Sesamia inferens (Walker, 1856) (Lepidoptera Noctuidae) is reported to infest many graminaceous crops and cause significant losses. S. inferens cause damage by killing the central shoot and producing a characteristic symptom called "dead heart". Since graminaceous crops are an important source of food for humans and their livestock, impetus should be given on designing efficient management strategies against pink stem borer. The study of genetic variability of pest populations enables to interpret the ecological investigations correctly and also helps to comprehend the dissimilar response of pest to management tactics. The present study was undertaken to evaluate the diversity in S. inferens populations using mitochondrial cytochrome oxidase subunit I sequences from India, Pakistan, China and Indonesia. Analysis revealed a very low nucleotide diversity in Indian populations (π = 0.00981), as compared to a high nucleotide diversity in the sequences outside India (π = 0.4989). The phylogenetic analysis also did not show any clustering among populations within India and Pakistan. However, the nearest neighbour for the Indian and Pakistan population is a sequence from Indonesia followed by China indicating possible ancestral background. This is the maiden attempt to assess the molecular diversity of Indian populations in comparison to populations from other Asian countries.
The online version contains supplementary material available at 10.1007/s13205-021-02678-y.
The online version contains supplementary material available at 10.1007/s13205-021-02678-y.Different levels of mannan oligosaccharides (MOs) (100, 300 and 500 mg kg-1) were incorporated into a basal diet to formulate three diets, which were used to test the growth performance and innate immunity of loaches. The basal diet without any MOs served as the control. Loaches fed with MO-containing diets for 70 days showed a higher specific growth rate, condition factor, survival rate, intestine weight index, intestine length index, intestine Lactobacillus population, intestine Bifidobacterium population, phenoloxidase activity, superoxide dismutase activity, glutathione peroxidase activity, acid phosphatase activity, alkaline phosphatase activity, lysozyme level, complement 3 and resistance to Aeromonas hydrophila than the loaches in the control group. The feed conversion ratio, intestine Escherichia coli population, malondialdehyde level, aspartate aminotransferase level and alanine aminotransferase level showed an opposite trend. The optimal dose of dietary MOs required for the maximum growth of loaches was 300 mg kg-1. Results indicated that dietary MOs promoted the growth performance and innate immunity of loaches and could be used as a dietary supplement for loaches.This study aimed to screen a potential anti-diarrheal probiotic for pigs to meet the growing demand for antibiotic alternatives in livestock. Nivolumab concentration Six intestinal pathogens, Escherichia coli (O157 H7) ATCC 43888, Staphylococcus aureus ATCC 6538, Listeria monocytogenes ATCC 19115, Salmonella Typhimurium ATCC 14028, Shigella boydii ATCC 9207, and Staphylococcus haemolyticus ZSY2 were employed as indicator bacteria. Our result showed that Lactobacillus animalis pZL8a isolated from pig feces had extensive and higher antibacterial activity against indicator pathogens among 9 tested strains. In addition, valuable attributes of pZL8a such as great tolerance of low pH (3.0) and bile salts (0.3%), high-level adhesion to Caco-2 cells, and similar susceptibility to the reference strain Lactobacillus rhamnosus GG (LGG) were observed. Compared with control, pZL8a supplement significantly improved the level of immunoglobulin G (IgG), immunoglobulin M (IgM), and interleukin-2 (IL-2) in mouse serum. Therefore, L. animalis pZL8a was proposed as a potential probiotic for further research and hope to reduce or replace the application of antibiotics in animal production.To gain a general understanding of the SOS system in Proteus species, in this study LexA-binding sites and the LexA regulons in 23 Proteus genomes were first predicted by phylogenetic footprinting server, then with Proteus vulgaris as an example, the expression of LexA regulon in iron limitation was investigated by proteomic analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR) method. The results showed that LexA proteins were highly conserved in Proteus species, and were in a close phylogenetic relationship with those in Gram-negative bacteria; the core SOS response genes lexA and recA were found in all the 23 genomes, indicating that this system was widely distributed in this genus; besides that, putative LexA-binding sites were also found in the upstream sequences of some genes involved in other biological processes such as biosynthesis, drug resistance, and stress response. Proteomic and RT-qPCR analyses showed that under iron deficient condition, the expression of lexA, recA and sulA was transcriptionally upregulated (p  less then  0.05), lexA was also translationally upregulated but recA was on the contrary (p  less then  0.05), whereas another SOS response gene dinI was transcriptionally downregulated (p  less then  0.01). These results indicated that in response to iron deficiency, the members of LexA regulon were not regulated by the same way, suggesting the existence of a precise regulation mechanism of SOS response in P. vulgaris. In conclusion, this study provided a preliminary understanding of the SOS system in Proteus species, which laid the foundation for further investigation of its roles in SOS response and other biological processes.A near-isogenic line (NIL) of the Indian wheat variety HD2733, carrying an introgressed Lr24/Sr24 genomic region was used for studying the effect of this introgression on quality traits. Data on the grain yield and 21 quality traits were recorded in this NIL and its recurrent parent (RP), both of which were grown in a randomized block design for two consecutive years. The statistical analysis revealed that grain yield was on par between the NIL and the RP. The NIL and its RP were both hard grained but the NIL showed a grain hardness index reduced by 9.7%. However, quality traits such as grain weight, protein content, sedimentation value, gluten traits, and solvent retention capacity were significantly higher in the NIL. The NIL also showed an increase in dough stability, a lower degree of softening and a higher farinograph quality number. These results indicated that the NIL could be utilized for hard grain, high protein and strong gluten-based products. An overall improvement in the quality of the NIL over its recurrent parent and without any yield penalty suggests that the Lr24/Sr24 genomic region could be gainfully utilized in wheat breeding for improving the industrial quality of wheat without jeopardising grain yield.
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