Notes

Igbo Presentation Surrogacy: Original Studies Based on the Oja Flute.
Sustainability Transitions (ST) is a complex phenomenon, encompassing environmental, societal and economic aspects. Its study requires a proper investigation, with the identification of a robust indicator and the definition of a suitable method of analysis. To identify the most informative geographical boundaries for analysing ST pathways, we consider the Carbon Emission Intensity (CEI) and estimate a four-level growth model to study its pattern over time for all the EU regions. We apply this model to a novel longitudinal dataset that covers CEI data of European regions at four different geographical scales (state, areas, regions, and provinces) over a nine-year timespan. This approach aims at supporting the decision-makers in developing more effective sustainability transitions policies across Europe, especially focusing on regions and overcoming the well-known "one-size fits all" approach.•The unconditional growth model has been applied to a multi-level structure considering four levels, defined by three geographical scales and time.•The ideal structure of the model would have required five levels, but the sample size of the dataset made the application computationally unfeasible;•The application of the model allowed to identify patterns of stability and change over time of the variable amongst different geographical units.Community falls in people with Parkinson's disease (PwPD) are common, costly, and often unanticipated. BMS-986158 solubility dmso Aside from static obstacles, it has been reported that oncoming people in community settings pose problems for PwPD when navigating. This suggests that PwPD may have difficulty (i) perceiving biological motion and action possibilities, and (ii) steering out of the way of oncoming persons. To date, laboratory research that investigated unanticipated steering manoeuvres in PwPD have only incorporated light- or arrow-based visual stimuli to simulate the spatiotemporal demands of these movements. However, such simple stimuli are not ecologically valid for examining biological motion perception and unanticipated steering manoeuvres used in avoiding oncoming people. To improve the generalisability of laboratory research in this field, a set of stereoscopic visual stimuli that feature an oncoming person initiating a sudden change in direction was developed for PwPD to engage with. Specifically, we modified and improved existing cinematographic techniques, software, and stereoscopic display technology to bring about•Ambulatory scenarios that were quasi-immersed with the laboratory environment.•Enhanced realism.•Better temporal consistency in video playback.Continuous slug flow in microreactors are featured by alternative presence of regulate segments of immiscible phases in microchannel or capillaries with lateral dimensions below 1 mm. Due to the high interfacial area and short diffusive distance therein, such microreactors have been widely applied in chemical engineering processes that are sensitive to mass transfer. Therefore, mass transfer rates in microreactors have long been broadly investigated via either typical offline or online methods. Compared to these conventional methods, the colorimetric technique based on the oxidation of resazurin with oxygen enables direct determination of physical mass transfer rates. However, this technique was currently applied only to the gas-liquid system in microreactors, and mostly in rectangular channels due to the simplicity in image processing. Based on this, the current paper showed a demo where the colorimetric technique using resazurin was adapted to a liquid-liquid system for the mass transfer study of flowing droplets within a slug flow capillary. Experimental tips and tricks were summarized, and a sliced color-concentration calibration strategy was proposed to balance analyzing efficiency and accuracy.•A detailed, practical description of a 2D lattice Boltzmann (LB) free-surface model and its coupling with a 1D LB shallow water model is provided.•A Python code is provided, that implements the Gaussian droplet benchmark of the research article (Thorimbert et al., 2019) corresponding to this method article.•Particular attention is given to the details of the free-surface implementation which, in the literature, vary among authors. These ambiguities must be addressed in order to build a reproducible scheme, as well as the exact implementation and parameters of the coupling model proposed in the associated research article.Archaeological faunal and botanical remains are often treated and published separately to understand past subsistence practices. This distinction is an arbitrary one based on methodological differences, especially since we know from ethnological sources that animal husbandry and crop cultivation are usually interdependent in agricultural systems. Here, we use correspondence, detrended correspondence, and canonical correspondence analyses to integrate these different lines of evidence. We customise this method by • Adjusting criteria to select and prepare data for integration. • Including independent parameters such as chronology and mean annual precipitation to study relationships. • Presenting additional visualisations of data to aid interpretation. The customised method we present can be applied to any time period, geographical region or research question, as long as botanical and faunal data are available. By analysing these data in an integrative way, we can improve our knowledge of subsistence and agriculture, which in turn can provide a context to better understand social and political changes in past societies.Plant canopies are wet for substantial amounts of time and this influences physiological performance and fluxes of energy, carbon and water at the ecosystem level. Leaf wetness sensors enable us to quantify the duration of leaf wetness and spatially map this to canopy structure. However, manually analysing leaf wetness data from plot-level experiments can be time-consuming, and requires a degree of subjective judgement in delineating wetness events which can lead to inconsistencies in the analysis. Here we•Describe how to set up an array of leaf wetness sensors (Phytos 31, Meter) enabling the measurement of leaf wetness duration through the profile of a forest canopy,•Present a method and R script to objectively identify and distinguish periods of rain and dew from the output of leaf wetness sensors,•Provide a criteria for separating the leaf wetness sensor output into dew and rain events which may form a reference standard, or be modified for use, in future studies.Neuroactive substances are the largest group of chemicals detected in European surface waters. Mixtures of neuroactive substances occurring at low concentrations can induce adverse neurological effects in humans and organisms in the environment. Therefore, there is a need to develop new screening tools to detect these chemicals. Measurement of behavior or motor effects in rodents and fish are usually performed to assess potential neurotoxicity for risk assessment. However, due to pain and stress inflicted on these animals, the scientific community is advocating for new alternative methods based on the 3R principle (reduce, replace and refine). As a result, the behavior measurement of early stages of zebrafish embryos such as locomotor response, photomotor response and spontaneous tail coiling are considered as a valid alternative to adult animal testing. In this study, we developed a workflow to investigate the spontaneous tail coiling (STC) of zebrafish embryos and to accurately measure the STC effect in the KNIME software. We validated the STC protocol with 3 substances (abamectin, chlorpyrifos-oxon and pyracostrobin) which have different mechanisms of action. The KNIME workflow combined with easy and cost-effective method of video acquisition makes this STC protocol a valuable method for neurotoxicity testing.•Video acquisition duration of 60 s at 25 ± 1 hpf was used•20 embryos exposed per dish and acclimatized for 30 min before video acquisition•Capability to inspect and correct errors for high accuracy.Validation of a survey instrument is an important activity in the research process. Face validity and content validity, though being qualitative methods, are essential steps in validating how far the survey instrument can measure what it is intended for. These techniques are used in both scale development processes and a questionnaire that may contain multiple scales. In the face and content validation, a survey instrument is usually validated by experts from academics and practitioners from field or industry. Researchers face challenges in conducting a proper validation because of the lack of an appropriate method for communicating the requirement and receiving the feedback. In this Paper, the authors develop a template that could be used for the validation of survey instrument. In instrument development process, after the item pool is generated, the template is completed and sent to the reviewer. The reviewer will be able to give the necessary feedback through the template that will be helpful to the researcher in improving the instrument.The encapsulation of growth factors is an important component of tissue engineer- ing. Using microspheres is a convenient approach in which the dose of factors can be regulated by increasing or decreasing the number of encapsulated microspheres. Moreover, microspheres offer the possibility of delivering the growth factors directly to the target site. However, the fabrication of microspheres by traditional emulsion methods is largely variable due to the experimental procedure. We have developed a protocol using a commercially available microfluidic system that allows formation of tunable particle-size droplets loaded with growth factors. The methodology includes a guide for preparing an alginate-growth factors solution followed by the specific set-up needed for using the microfluidic system to form the microspheres. The pro- cedure also includes a unique post-crosslinking process without pH modification. These methods allow the preservation of integrity and bioactivity of the growth factors tested (BMP-6 and TGFβ -3) and their subsequent sustained delivery.•The protocol can be tuned to form particles of various sizes.•The gentle post-crosslinking process allows conformational integrity of various bioactive molecules.Accelerated reliability testing of integrated circuit (IC) packages, such as wire-bonded devices, is a useful tool for predicting the lifetime corrosion behavior of real-world devices. Standard tests, such as highly accelerated stress test, involves subjecting an encapsulated device to high levels of humidity and high temperature (commonly 85-121 ⁰C and 85-100% relative humidity). A major drawback of current reliability tests is that mechanistic information of what occurs between t = 0 and device failure is not captured. A novel method of in-situ investigation of the device corrosion process was developed to capture the real time mechanistic information not obtained in standard reliability testing [1]. The simple, yet effective methodology involves•Immersing a micropattern or device directly into contaminant-spiked aqueous solution, and observing its morphological changes under optical microscope paired with a camera.•Short (2-48 h) time required for testing (compared to 24-300 h of standard tests).•No need for humidity chambers.
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