Notes

Hand in hand Inactivation of Microorganisms Utilizing a Mix of Erythorbyl Laurate and Ultra violet Type-A Gentle Treatment method.
The past four decades have brought significant and increasingly rapid changes to the world of instrument design, fabrication, and availability due to the emergence of 3D printing, open-source code and equipment, and low-cost electronics. These, along with other technological advances represent a nexus in time ripe for the wide-spread production and availability of low-cost sophisticated scientific equipment. To that end, the design of a 3D printable and open-source, modular spectrometer is described. This specific instrument is distinctly different from others that have been reported in recent years in that it was designed outside of the "black box" paradigm of nearly all other commercially available and open-source spectrometers. This feature along with its design to be produced on low-end consumer-grade 3D printers and with parts available at nearly any local hardware store allow this instrument to further erode current barriers to instrument access. Additionally, the use cases presented here demonstrate similar capabilities to commercially available equipment at a fraction of the cost.Here we present a versatile system for milliliter-scale perfusion culture of adherent cells that can be built using basic tools, based on a readily available one-well culture plate (84 cm2 culture area). Media composition and flow paths can be programmatically controlled via USB serial interface using the FETbox hardware controller and associated PlateFlo Python package. The FETbox can control up to five high current 12 V devices such as common pinch valves, solenoids, and DC motor peristaltic pumps. It was designed to be easily customized with built-in accommodation for additional electronic components (e.g. analog sensors and input), use of the ubiquitous Arduino Nano platform, and easily expanded serial communication protocol. Multiple FETboxes can be used in parallel for additional devices. Applications of the PlateFlo system include perfusion culture of laboratory experiments requiring large cell numbers including genome-scale genetic screens and proteomics, as well as novel perfusion schemes including dynamic media conditions and sequential cell culture.Benchtop nuclear magnetic resonance (NMR) spectrometers are versatile analytic instruments with low acquisition and operation cost. However, in the basic version, samples must be manually measured one after the other. We herein describe the open-source autosampler RotoMate that allows the automated operation of such instruments. The hardware is easily assembled from 3D-printed and inexpensive off-the-shelf parts, and is controlled by an Arduino Uno. A software package interlinks the operation of the autosampler with the software of the NMR spectrometer and the software for the processing of the spectra. Experiments for up to 30 samples can be inserted into an interactive sample list. The autosampler automatically inserts and ejects the samples, initiates measurements on the spectrometer according to parameters specified in the sample list, and interacts with a common NMR software in the processing and visualization of the obtained spectroscopic raw data. If an internal standard is present, conversions and yields of chemical reactions are automatically calculated, enabling e.g. the monitoring of reactions. The device was fitted to a Magritek Spinsolve instrument and can interact with a free academic version of ACD NMR software to process the spectra, but can likely be adapted to similar instruments and spectroscopy software packages.The rapid warming of our planet has resulted in accelerated melting of ice in polar regions. Currently we have limited knowledge on how, where and when the surface meltwater layer is mixed with the underlying ocean due to lack of observations in these remote areas. We present a lightweight (17 kg) and low-cost (6000€) instrument for autonomous profiling across the strongly stratified upper layer in Arctic coastal waters, freshened by the riverine input and meltwater from glaciers, icebergs, and sea ice. The profiler uses a specially designed plunger buoyancy engine to displace up to 700 cm3 of water and allows for autonomous dives to 200 m depth. It can carry different sensor packages and convey its location by satellite communication. Two modes are available (a) a free-floating mode and (b) a moored mode, where the instrument is anchored to the seafloor. In both modes, the profiler controls its velocity of 12 ± 0.3 cm/s resulting in 510 ± 22 data points per 100 m depth. Equipped with several sensors, e.g. conductivity, temperature, oxygen, and pressure, the autonomous profiler was successfully tested in a remote Northeast Greenlandic fjord. Data has been compared to traditional CTD instrument casts performed nearby.[This corrects the article DOI 10.1016/j.ohx.2018.e00044.][This corrects the article DOI 10.1016/j.ohx.2021.e00195.][This corrects the article DOI 10.1016/j.ohx.2020.e00137.][This corrects the article DOI 10.1016/j.ohx.2020.e00139.][This corrects the article DOI 10.1016/j.ohx.2018.e00026.][This corrects the article DOI 10.1016/j.ohx.2019.e00080.][This corrects the article DOI 10.1016/j.ohx.2020.e00130.][This corrects the article DOI 10.1016/j.ohx.2020.e00131.][This corrects the article DOI 10.1016/j.ohx.2020.e00135.][This corrects the article DOI 10.1016/j.ohx.2016.07.001.][This corrects the article DOI 10.1016/j.ohx.2018.e00042.][This corrects the article DOI 10.1016/j.ohx.2020.e00129.].An ad hoc autonomous mobile microgrid system requires electrical connections to be formed between physically separated resources. This work proposes the use of unmanned ground vehicles (UGVs) as the means to deploy the electrical cable that creates these connections. This operation requires careful control of the cabling at variable speeds to avoid entanglement with the deploying UGV or obstacles in complex outdoor environments. Searching for a product that could supply the needed control and flexibility revealed a lack of compact and low-cost options. Existing options are very heavy ( > 100 lbs) and do not supply precision in their deployment. There is no commercial off-the-shelf option available for small-scale cable deployment operations with size and weight constraints. To fulfill the application requirements and to combat this deficiency, a custom design and build of an "Adjustable Cable Management Mechanism" (ACMM) was required. This ACMM provides a low cost, compact platform for powered and controlled deployment and retraction of different-sized cable under moderate loads, utilizing Commercial Off-The-Shelf components (COTS). Employing this design has enabled a variety of tasks that require distribution of electrical or data cables to be accomplished for small-scale projects. learn more The goal of this paper is to give detailed design specifications of the ACMM and instructions on how to recreate it and calibrate it to be useful for tethering robots in various applications such as steep terrain, internet connection through tight spaces, or electrical connection between nodes for complex microgrids.We present a datalogger based on Arduino cards and commercially available tools for radio frequency identification, which we term the e-RFIDuino. Designed to be robut, easy to build and install, it detects and records the mobility of objects tagged with active transponders emitting in the ultra-high frequency domain (433.5 MHz). It functions without connection to the power supply network and is adapted to harsh outdoor environments. Once installed in the field and its on-site sensing field is determined, the data collected (timestamp of detection, transponder identification number, and received signal strength indication) allow estimation of the virtual velocity of tracer passage and investigation of displacement patterns at the scale of the area of detection. Experimental tests showed the device to have very high effectiveness when used to monitor the passage of sediment tracers in a torrential river system during various flood events over several months. The total cost to construct this open source device is below 850 Euros, and it is easily customizable. In the future, it could be equipped with a system for data transmission over the mobile telephone network to reduce the field effort and time required to obtain data, and to provide real-time triggering of field acquisitions at the most appropriate times.Here we describe a completely integrated and customizable microfluidic control and sensing architecture that can be readily implemented for laboratory or portable chemical or biological control and sensing applications. The compact platform enables control of 32 solenoid valves, a multitude of pumps and motors, a thermo-electric controller, a pressure controller, and a high voltage power supply. It also features two temperature probe interfaces, one relative humidity and ambient temperature sensor, two pressure sensors, and interfaces to an electrical conductivity sensor, flow sensor, and a bubble detector. The platform can be controlled via an onboard microcontroller and requires no proprietary software.For some IoT applications, mobile entities are considered as the main source of sensed data, requiring the attachment of sensor modules on them. The endowing of sensing capabilities to such mobile entities can be performed in different ways, but the adoption of a reference hardware framework can bring a series of advantages, specially in dynamic complex scenarios. This article exploits the MSensorMob2 multi-sensor hardware framework for monitoring in areas with disconnection periods, comprising sensing, transmission and reconfiguration functions. Comprehensive analyses on multiple open-source hardware platforms are conducted, assessing their costs, deployment constraints and performance issues when implementing this development framework.Water sampling is an essential undertaking for water utilities and agencies to protect and enhance our natural resources. The high variability in water quality, however, often necessitates a spatially distributed sampling program which is impeded by high-cost and large sampling devices. This paper presents the BoSL FAL Pump - a low-cost, easily constructed, 3D-printed peristaltic pump which can be made from commonly available components and is sized to suit even the most space constrained installations. The pump is 38 mm in height and 28 mm in diameter, its components cost $19 AUD and the construction time is just 12 min (excluding 3D printing times). The pump is driven by a direct current motor which is commonly available, cheap and allows for flexibility in the energy supply (5-12 V). Optionally, the pump has a Hall effect sensor and magnet to detect rotation rates and pumping volumes to improve the accuracy of pumping rates/volumes. The pump can be easily controlled by commonly available microcontrollers, as demonstrated by this paper which implements the ATmega328P on the Arduino Uno R3. This paper validates the pump for long-term deployments at flow rates of up to 13 mL per minute in 0.14 mL volume increments at accuracy levels of greater than 99%. The pump itself is scalable, allowing for a wider range of pumping rates when, for example, large volume samples are required for pathogen and micropollutant detection.
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