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Budgerigar is a common name for a colorful Australian native bird belonging to the Melopsittacus undulatus species. It is a very familiar pet around the world and its breeding has been grown in Iran. This study was conducted on a 2-year-old budgerigar with a nodular mass on the left wing. Physical examination revealed a firm, round and well-circumscribed mass approximately 1.70 cm in diameter. Radiographs showed a soft tissue mass with no involvement of bony structures. selleck chemicals llc Fine needle aspiration was performed and the sample was cultured. In cultural examination, Klebsiella spp. were isolated in pure culture. Genus and species of the bacteria were confirmed using multiplex polymerase chain reaction. The mass was surgically excised and it was mainly composed of numerous, large lipid-laden macrophages containing abundant vacuolated cytoplasm, extracellular acicular cholesterol clefts and large number of multinucleated giant cells (especially multinucleated Touton giant cells) in the dermis. Finally, a diagnosis of cutaneous xanthogranuloma was made based on histopathological findings. © 2019 Urmia University. All rights reserved.Canine osteosarcoma (OS) or osteogenic sarcoma is an aggressive tumor of the skeletal system, associated with a rapid progression and guarded prognosis. The osteosarcomas, mostly arise from the appendicular skeleton while axial OS (osteosarcoma of flat bones) are less reported in the majority of large breeds. This report describes complete para-clinical investigations of an aggressive chondroblastic OS involving facial flat bones with highly metastatic characterization in a large mix breed stray dog. Radiographic and computed tomography findings demonstrated an amorphous and active new bone formation, associated with the severe lytic areas in the left maxillary, orbital and zygomatic bones. Also, lots of nodular densities were distributed in all lung lobes. The cytological examination of the mass revealed individualized oval to spindle-shaped pleomorphic mesenchymal cells exhibiting many criteria of malignancy such as marked anisocytosis, anisokaryosis, prominent and multiple nucleoli. The punctate cytoplasmic vacuoles were obvious and bi-nucleated cells were frequently observed. These cells were seen in the background of an eosinophilic matrix. Histopathologic evaluation of the mass exhibited areas of osseous differentiation within the mass containing bony spicules and wavy bone formation along with the vast areas of cartilaginous differentiations with chondroblasts in lacunar spaces. Ultimately, chondroblastic OS with severe lung metastasis was confirmed and the animal was euthanized. © 2019 Urmia University. All rights reserved.Nowadays, the consumption of meat and meat products has been increased with modern manufacturing techniques. Due to the economic value, the likelihood of using unauthorized tissue is possible in meat products. The aim of this study was to apply morphological methods for detection of unauthorized tissues in meat sausage. In this study, a total number of 20 samples of different types of sausages were randomly collected from markets, in north-east of Iran. Each sample was divided into three equal parts and three paraffin-embedded blocks were prepared from each part (180 blocks). Then the sections were stained using Hematoxylin and Eosin, Masson's trichrome, Periodic acid- Schiff/Alcian blue and Verhoeffe/Van Gieson. A total number of 720 slides were observed using a light microscope. This research showed the use of unauthorized tissues in the sausages which was detected by histological methods. We observed authorized tissues like skeletal muscle fiber (100%), fat tissue (100%) and plant material (97.70%). A wide range of unauthorized tissues were detected including dense connective tissue (6.66%), cartilage (28.30%), bone (8.30%), skin (51.60%), smooth muscle (1.66%) and blood vessels (11.66%). The results of this study confirmed the use of unauthorized tissue in meat sausages in Iran and concluded that the histological methods especially Masson's trichrome staining are a practical technique for routine assessment of authenticity and quality of sausage to protect the consumers from adulteration. © 2019 Urmia University. All rights reserved.This study investigated the toxic effects of dried pulverized Caloncoba echinata leaves on the hematology, blood biochemistry and vital organs of male albino rats. Twenty adult male rats were randomly divided into four groups of five rats each. Groups A, B and C were fed 25.00%, 15.00% and 5.00% of pulverized C. echinata leaves in feed respectively while group D was given normal feed for a four weeks period. Blood samples were collected at two weeks intervals for hematological and blood biochemistry analyses. Results showed a significant reduction of packed cell volume, hemoglobin concentration and total red blood cell counts in group B from week two to the end of the study. There was also a reduction of body weight and leukocytosis in groups A and B from week two to the end of the study. There was a significant reduction of albumin in group B when compared to the other groups after two weeks and a significant reduction in blood glucose concentration in group A after two weeks of the feeding of the leaves to the end of the study. Discrete areas of degeneration and necrosis of hepatocytes were observed in the rats of groups A and B and testicular atrophy in group B rats. It was concluded that feeding the rats with the pulverized C. echinata leaves led to a significant reduction of body weights and erythrocytic parameters, leukocytosis, hepatic and testicular injuries in the albino rats. © 2019 Urmia University. All rights reserved.Whole soybeans serve as one of the main sources of protein in ruminant nutrition. Different processing methods have been employed for ruminal protein protection. The present study was conducted to determine the effects of microwave irradiation [900 W; 2, 4 and 6 min] on quality, ruminal degradability and estimated in vitro intestinal digestibility of availability soybean crude protein. This experiment was performed in a completely randomized design with seven treatments including control (no processing), along with 2, 4 and 6 min of microwave irradiation on whole and ground soybeans. Protein and carbohydrate fractions were determined according to Cornell Net Carbohydrate and Protein System (CNCPS). Triplicates of the samples were incubated in the rumen of three cannulated Holstein steers for up to 48 hr. Microwave irradiation increased neutral detergent insoluble nitrogen, metabolizable protein content and resulted in a lower effective rumen degradability and in vitro gas production. Nevertheless, longer processing time led to higher unavailable protein and carbohydrate fractions.
Read More: https://www.selleckchem.com/products/Vorinostat-saha.html
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