Notes

TEMPERATURE: 18-25°C (ROOM TEMPERATURE)


REPORTING FORMAT:
REFERENCE:
WHO LABORATORY MANUAL FOR THE EXAMINATION AND PROCESSING OF HUMAN SEMEN 5th EDITION.

GROSSLY SEMEN SAMPLE CONTAINS GELATINOUS CLUMPS
AGGREGATION OF SPERM CELLS WITH GERM CELLS SEEN
Temperature:18-25 °C. (ROOM TEMPERATURE)

FOR AZOSPERMIA SAMPLE LOW QUANTITY:
ON INITIAL SLIDE THERE IS NO SPERMATOZOA SEEN IN 100 FIELDS SUGGESTIVE OF AZOOSPERMIA, CENTRIFUGED METHOD COULD NOT BE POSSIBLE DUE TO LOW VOLUME.

PLEASE CORRELATE WITH THE PATIENT'S CLINICAL HISTORY.

FOR AZOSPERMIA SAMPLE:
ON INITIAL SLIDE THERE IS NO SPERMATOZOA SEEN IN 100 FIELDS SUGGESTIVE OF AZOOSPERMIA, TEST IS CONFIRMED BY CENTRIFUGED METHOD. PLEASE CORRELATE WITH THE PATIENT'S CLINICAL HISTORY.



FOR SEVERE OLIGOSPERMIA SAMPLE BY CENTRIFUGED METHOD:
ON INITIAL SLIDE THERE IS ONLY 02 MORPHOLOGICALLY ABNORMAL AND NON-MOTILE SPERMS SEEN IN 100 FIELDS, AFTER CENTRIFUGED METHOD THE WHOLE SAMPLE SHOWS ONLY 17 MORPHOLOGICALLY ABNORMAL AND NON-MOTILE SPERMS.PLEASE CORRELATE WITH THE PATIENT'S CLINICAL HISTORY.





ON INITIAL SLIDE THERE ARE ONLY 04 MORPHOLOGICALLY ABNORMAL AND NON-MOTILE SPERMS SEEN IN 100 FIELDS, AFTER CENTRIFUGED METHOD THE WHOLE SAMPLE SHOWS ONLY 25 MORPHOLOGICALLY ABNORMAL SPERMS, IN WHICH 01 IS MOTILE AND 24 ARE NON-MOTILE SPERMS.PLEASE CORRELATE WITH THE PATIENT'S CLINICAL HISTORY.



FOR CRYPTOZOOSPERMIA SAMPLE BY CENTRIFUGED METHOD:
ON INITIAL SLIDE THERE IS NO SPERMATOZOA SEEN IN 100 FIELDS, AFTER CENTRIFUGED METHOD THE WHOLE SAMPLE SHOWS ONLY 01 MORPHOLOGICALLY ABNORMAL AND NON-MOTILE SPERM.PLEASE CORRELATE WITH THE PATIENT'S CLINICAL HISTORY.


ON INITIAL SLIDE THERE IS NO SPERMATOZOA SEEN IN 100 FIELDS, AFTER CENTRIFUGED METHOD THE WHOLE SAMPLE SHOWS ONLY 20 MORPHOLOGICALLY NORMAL & ABNORMAL AND ALL NON-MOTILE SPERMS. PLEASE CORRELATE WITH THE PATIENT'S CLINICAL HISTORY.

FOR CRYPTO MOTILE:

ON INITIAL SLIDE THERE IS NO SPERMATOZOA SEEN IN 100 FIELDS, AFTER CENTRIFUGED METHOD THE WHOLE SAMPLE SHOWS ONLY 07 MORPHOLOGICALLY ABNORMAL AND NON-MOTILE SPERM IN WHICH 01 MOTILE SPERM CELL SEEN.PLEASE CORRELATE WITH THE PATIENT'S CLINICAL HISTORY.


VIABILITY TEST RESULT FOR < 40 % MOTILITY.




NOTE:

DUE TO LOW PERCENTAGE OF MOTILITY <40% VIABILITY TEST IS PERFORMED, VIABILITY TEST RESULT SHOWS:

LIVE SPERMS: 40%

DEAD SPERMS: 60%

PLEASE CORRELATE WITH THE PATIENT CLINICAL HISTORY.












VIABILITY TEST RESULT FOR 100% NON-MOTILE SPERMS %:




NOTE:

DUE TO 100% NON-MOTILE PERCENTAGE OF MOTILITY VIABILITY TEST IS PERFORMED,

VIABILITY TEST RESULT SHOWS:

LIVE SPERMS: 05%

DEAD SPERMS: 95%

PLEASE CORRELATE WITH THE PATIENT CLINICAL HISTORY.


VIABILITY TEST RESULT FOR 100% NON-MOTILE SPERMS %:




FOR AGGLUTINATION:


NOTE: ON INITIAL SLIDE, DEGREE OF AGGULUTINATION (3+) large (>50 SPERM/ AGGLUTINATE FREE SPERM), TAIL-TIP-TO-TAIL-TIP SEEN.


For pap stain comment:

NOTE: NO.OF ROUND CELLS (GERM CELLS AND POLYMORPHONUCLEAR LEUKOCYTES) ARE CONFIRMED BY PAPANICOLAOU STAIN HPF. PLEASE CORRELATE WITH THE PATIENT’S HISTORY.MICROBIOLOGICAL CORRELATION SUGGESTED.










FOR BACTERIA:
NOTE:

3+++
Prescence of bacteria in semen sample required correlation of microbiological evaluation.



LOW COUNT VIABILITY FORMAT:


NOTE: PERCENTAGE OF MOTILE SPERM IS <40%, DUE TO LOW COUNT OF SPERM CELLS VIABILITY TEST COULD NOT BE POSSIBLE.




NOTE: PERCENTAGE OF NON- MOTILE SPERMS IS 100%, DUE TO LOW COUNT OF SPERM CELLS VIABILITY TEST COULD NOT BE POSSIBLE.



THIS TEST WAS PERFORMED ON SEMI-AUTOMATED SPERM CLASS ANALYZER (CASA) AND THE REFERENCE IS WHO LABORATORY MANUAL FOR THE EXAMINATION AND PROCESSING OF HUMAN SEMEN 5TH EDITION (2010)




DIAGNOSIS: NORMOZOOSPERMIA (F8)

DIAGNOSIS: OLIGOZOOSPERMIA (F9)

DIAGNOSIS: ASTHENOZOOPSERMIA (F10)

DIAGNOSIS: OLIGOASTHENOZOOPSERMIA (F12)

DIAGNOSIS: TETRATOZOOSPERMIA

DIAGNOSIS: OLIGOTERATOZOOSPERMIA

DIAGNOSIS: ASTHENOTERATOZOOSPERMIA

DIAGNOSIS: OLIGOASTHENOTERATOZOOSPERMIA