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Rats (n= 6) of control group were treated orally with
vehicle (3 ml/kg) of 0.3% CMC and those of the test groups
with MHN (50, 100 and 200 mg/kg) once a day for 5 consecutive
days. Phenylbutazone (100 mg/kg, oral) was administered
as a positive control. On the 5th day, 1 h after drug
treatment, 1% carrageenin was injected into the plantar tissue
of the right hind paw [11]. The contra-lateral hind paws were
injected with 0.1 ml of saline as control. Paw volume was
measured plethysmographically at 0 and 3 h after carrageenin
injection.
Percentage inhibition of oedema was calculated as follows:
%inhibition = ð1−VT = VCÞ × 100
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