Notes

These results provide some basis for their large-scale production and further application as alternatives to mammalian Collagen alpha B chain: increased proportion in human atherosclerosis
In a study of human atherosclerotic plaques, the relative abundance of alpha chains in pepsin-solubilized collagens from 28 human aortas was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ratio of alpha B, a component of the alpha chain in type V collagen, to alpha 1(I) was markedly increased in the atherosclerotic plaques compared to the nonsclerotic intact media and adventitia. It is suggested that proliferating smooth muscle cells in the sclerotic lesion were transformed to synthesize a larger amount of collagen alpha B chain during the process of human atherogenesis.Human edentulous ridge collagens. Characterization and comparison with gingival Narayanan AS, Hassell TM, Page RC, Hoke JA, Meyers D.[Selective disorders of the cross-linking of collagen].


Estrogen induces different responses in dermal and lung fibroblasts: special Hosokawa M, Ishii M, Inoue K, Yao CS, Takeda T.Fibroblast-like cells derived from dorsal skin and lung of AKR strain mouse embryos were cultured with estrogen for 4 days. The labeled hydroxyproline content was measured as newly synthesized collagenous protein in the cell layer and media. Although collagen synthesis by both cell lines was increased in both fractions under the influence of beta-estradiol-3-benzoate, at physiological concentrations of 10(-3)-10(-1) microgram/ml, the rate of increase differed. Fibroblasts derived from skin showed increased collagen synthesis of approximately 76%, while those from lung showed an increase of approximately 25%. Total protein synthesis by both cell lines also increased. In lung fibroblast cultures the synthesis of total protein was increased more than the synthesis of collagens; on the other hand in skin fibroblast cultures the synthesis of collagen was increased more than the synthesis of total protein.

DNA synthesis by both cell lines was not affected by estrogen at the concentrations used. These findings suggest that fibroblasts develop in a Antimicrobial Potency Against Planktonic And Biofilm MRSA.University, Kweishan, Taoyuan, Taiwan.Gung University, Kweishan, Taoyuan, Taiwan.Herbal Medicine, Chang Gung University of Science and Technology, Kweishan, BACKGROUND: It is important to explore the interaction between antibacterial nanoparticles and microbes for understanding bactericidal activity and developing novel applications. It is possible that the nanoparticulate size can PURPOSE: The purpose of this study was to evaluate the antimicrobial and antibiofilm properties of cetylpyridinium chloride (CPC)-decorated nanoemulsions against methicillin-resistant Staphylococcus aureus (MRSA).METHODS: The droplet size could be adjusted by varying the percentage of squalene, the main ingredient of the oily core.

RESULTS: We fabricated cationic nanoemulsions of three different sizes, 55, 165, and 245 nm. Order immediately showed greater storage stability than the self-assembled CPC micelles. The tested nanoemulsions exhibited more antimicrobial activity against Gram-positive bacteria than Gram-negative bacteria and fungi. The killing of MRSA was mainly induced by direct cell-membrane damage. This rupture led to the leakage of cytoplasmic DNA and proteins. Buy now might also degrade the DNA helix and disturb protein synthesis. The proteomic analysis indicated the significant downregulation of DNA-directed RNA polymerase (RNAP) subunits β and β'.

The antibacterial effect of nanoemulsions increased with decreasing droplet size in the biofilm MRSA but not planktonic MRSA. The small-sized nanoemulsions had potent antibiofilm activity that showed a colony-forming unit (CFU) reduction of 10-fold compared with the control. The loss of total DNA concentration also negatively correlated CONCLUSION: The present report established a foundation for the development of Conflict of interest statement: The authors report no conflicts of interest in Kinetic study on the reaction of cisplatin with metallothionein.Pediatrics, 750 East Adams Street, Syracuse, NY 13210, USA.The binding of cisplatin to metallothionein (MT) was investigated at 37 degrees C in 10 mM Tris-NO3 (pH approximately 7) and 42 mM NaCl. The conditions were chosen to mimic passage of clinical concentrations of cisplatin through the cytosol. The reactions were monitored by high-performance liquid chromatography (HPLC), atomic absorption spectroscopy, and ultraviolet (UV) absorption spectroscopy.

The UV data showed that several reactions occur, the first of which does not affect the absorbance (no Pt-sulfur bond formation). They also suggested that if [cisplatin] is large compared with [MT], the rate of subsequent reaction is between first and second order in [cisplatin] and between zeroth and first order in [MT]. HPLC eluates with 24 < retention time (tR) < 27 min contained undialyzable Pt, which increased with reaction time and corresponded to Pt-thionein product. Eluates with 3 < tR < 7 min corresponded to unbound cisplatin and allowed determination of second-order rate constants (k), using the second-order rate equation. The k value for cisplatin reacting with apo-MT was approximately 04 M-1 s-1, Cd/Zn-MT approximately 05 M-1 s-1, Cd7-MT approximately 03 M-1 s-1, and Zn7-MT approximately 05 M-1 s-1. Thus, cisplatin displaced Cd and Zn equally well.

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