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Addition Ltp Rats Control Animals Adult Animals
Oral administration of 2'-FL exclusively during lactation enhanced cognitive abilities, not only in childhood but also in adulthood.Enzymatic synthesis of β-galactosyl fucose using recombinant bifidobacterial β-galactosidase and its prebiotic effect.National University, Seoul, 151-742, Republic of Korea.Hee University, Yongin, 174, Republic of Korea.Breast-fed infants have Bifidobacterium-rich gut microbiota compared to infants fed formula. Fucosylated oligosaccharides are the major components of human milk oligosaccharide (HMO) which confer various beneficial effects including prebiotic effect and protection from pathogenic infection on the host.

A novel prebiotics was developed using bifidobacterial β-galactosidase and fucose and lactose as substrates. Structure analysis revealed it as β-D-galactopyranosyl-(1 → 3)-O-L-fucopyranose named as β-galactosyl fucose α1-4 linkages. Among the four Lactobacillus strains examined, all but L. delbrueckii subsp. bilgaricus KCTC 3635 grew better on gal-fuc than on β-GOS. Among the 11 bifidobacterial species examined, all except for B. bifium used gal-fuc as much as GOS.

Moreover, the gal-fuc was noticeably better used by Bifidobacterium infantis, the major intestinal bacteria of breast fed infant. Among 15 non-probiotic bacteria, only 4 strains used gal-fuc better than β-GOS. In conclusion, a novel gal-fuc is expected to contribute to beneficial changes of gut microbiota. Graphical abstract A novel form of β-galactosyl fucose with High-Fructose Corn-Syrup-Sweetened Beverage Intake Increases 5-Hour Breast Milk Fructose Concentrations in Lactating Women.This study determined the effects of consuming a high-fructose corn syrup concentrations in lactating women. At six weeks postpartum, lactating mothers (n = 41) were randomized to a crossover study to consume a commercially available HFCS-sweetened beverage or artificially sweetened control beverage. At each session, mothers pumped a complete breast milk expression every hour for six consecutive hours.

The baseline fasting concentrations of breast milk fructose, glucose, and lactose were 5 ± 1 µgmL, ± mgmL, and 6 ± 1 gdL, respectively. The changes over time in breast milk sugars were significant only for fructose (treatment × time, p 1). Post hoc comparisons showed the HFCS-sweetened beverage vs. control beverage increased breast milk fructose at 1 min (8 ± 2 vs. 5 ± 1 µgmL), 1 min (9 ± 1 vs. 5 ± 2 µgmL), 2 min (7 ± 1 vs. 5 ± 1 µgmL), and 0 min (6 ± 1 vs.

4 ± 1 µgmL) (all p 5). The mean incremental area under the curve for breast milk fructose was also different between treatments (14 ± 1 vs. -2. ± 1 µgmL × 3 min, p 1). There was no treatment × time interaction for breast milk glucose or lactose. Our data suggest that the consumption of an HFCS-sweetened beverage increased breast milk fructose concentrations, which remained elevated up to five hours post-consumption.Conflict of interest statement The authors declare no conflict of interest.

Seebio 2'-fucosyllactose founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in Biochemical characterization of a novel α-L-fucosidase from Pedobacter sp. and its application in synthesis of 3'-fucosyllactose and 2'-fucosyllactose.College of Food Science and Nutritional Engineering, China Agricultural University, No7 Qinghua Donglu, Haidian District, Beijing, 83, China.College of Engineering, China Agricultural University, No7 Qinghua Donglu, Haidian District, Beijing, 83, China.College of Food Science and Nutritional Engineering, China Agricultural University, No7 Qinghua Donglu, Haidian District, Beijing, 83, China. Fucosyllactoses have gained much attention owing to their multiple functions, including prebiotic, immune, gut, and cognition benefits. In this study, human milk oligosaccharide (HMO) 2'-fucosyllactose (α-L-Fuc-(1,2)-D-Galβ-1,4-Glu, 2'FL) and its isomer 3'-fucosyllactose (α-L-Fuc-(1,3)-D-Galβ-1,4-Glu, 3'FL) with potential prebiotic effect were synthesized efficiently by a novel recombinant α-L-fucosidase.

An α-L-fucosidase gene (PbFuc) from Pedobacter sp. CAU9 was successfully cloned and expressed in Escherichia coli (E. coli). The deduced amino acid sequence shared the highest identity of 36% with the amino sequences of other reported α-L-fucosidases. The purified α-L-fucosidase (PbFuc) had a molecular mass of kDa. The enzyme exhibited specific activity and 2'FL (3 Umg). It showed the highest activity at pH 5 and 35 °C, respectively.
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