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Firm: Simply what does It Imply to become Man?
Embryonic neural stem cells (NSCs), comprising neuroepithelial and radial glial cells, are indispensable precursors of neurons and glia in the mammalian developing brain. Since the process of neurogenesis occurs in a hypoxic environment, the question arises of how NSCs deal with low oxygen tension and whether it affects their stemness. Genes from the hypoxia-inducible factors (HIF) family are well known factors governing cellular response to hypoxic conditions. In this study, we have discovered that the endogenous stabilization of hypoxia-inducible factor 1α (Hif1α) during neural induction is critical for the normal development of the NSCs pool by preventing its premature depletion and differentiation. The knock-out of the Hif1α gene in mESC-derived neurospheres led to a decrease in self-renewal of NSCs, paralleled by an increase in neuronal differentiation. see more Similarly, neuroepithelial cells differentiated in hypoxia exhibited accelerated neurogenesis soon after Hif1α knock-down. In both models, the loss of Hif1α was accompanied by an immediate drop in neural repressor Hes1 levels while changes in Notch signaling were not observed. We found that active Hif1α/Arnt1 transcription complex bound to the evolutionarily conserved site in Hes1 gene promoter in both neuroepithelial cells and neural tissue of E8.5 - 9.5 embryos. Taken together, these results emphasize the novel role of Hif1α in the regulation of early NSCs population through the activation of neural repressor Hes1, independently of Notch signaling. In strawberry, sucrose is the major form of carbohydrate translocated from the leaves to the fruits and plays an important role in fruit ripening. As a conserved energy sensor, sucrose nonfermenting-1 (SNF1)-related kinase 1 (SnRK1) plays an important role in plant carbon metabolism. However, evidence that SnRK1 regulates sucrose accumulation in fruits is lacking. In this study, we transiently expressed FaSnRK1α in strawberry fruits and found that overexpression (OE) of the FaSnRK1α gene significantly increased the sucrose content, whereas repression of FaSnRK1α by RNA interference (RNAi) decreased the sucrose content. Further analysis revealed that FaSnRK1α increased the expression of FaSUS1 and FaSUS3 as well as the activity of sucrose synthase (SUS; EC 2.4.1.13) and that FaSPS1 expression and sucrose phosphate synthase (SPS; EC 2.4.1.14) activity were strongly downregulated, which decreased the accumulation of sucrose. However, the expression of FaSPS3, which is reported to contribute to sucrose accumulation, was induced by FaSnRK1α, and FaNI expression and invertase (INV; EC 3.2.1.26) activity were upregulated by FaSnRK1α. In addition, FaSnRK1α positively upregulated the expression of the sucrose transporter (SUT) genes FaSUT1 and FaSUT5 and interacted with FaSUS1, FaSPS1 and FaSPS3 proteins but not with FaSUS3, FaNI, FaSUT1 or FaSUT5 proteins. Overall, FaSnRK1α systematically regulates the expression of the genes and activities of key enzymes involved in the sucrose metabolic pathway and promotes the long-distance transport of sucrose, thereby increasing sucrose accumulation and ultimately promoting fruit ripening. However, the mechanisms by which sucrose transport and degradation are regulated by SnRK1 warrant additional research. INTRODUCTION Primary cardiac angiosarcoma is rare, and its prognosis remains poor. Complete surgical resection is the first choice among the available treatments, to ensure prolonged patient survival. PRESENTATION OF CASE We report the case of a 72-year-old man with right atrial angiosarcoma who presented with bloody pericardial effusion due to tumor hemorrhaging. Emergency surgery was performed. The right atrial free wall and vena cava were reconstructed with pedicled autologous pericardium. The patient died 12 months later because of multiple organ failure due to metastasis. Autopsy showed a maintained right atrial chamber and only mild calcification. DISCUSSION Pedicled autologous pericardium may have contributed to preserving cardiac function that could withstand chemotherapy and radiotherapy. CONCLUSION Pedicled autologous pericardium was found to be useful for reconstructing the cardiac chamber after removal of a cardiac tumor. INTRODUCTION A parameatal urethral cyst is a rare finding in an otherwise well child. They can present as either congenital malformations or as a finding later in life, and may occur either unilaterally or bilaterally on the glans penis. Though they are benign, possible complications include impaired urinary stream flow, dysuria, or hindered cosmesis. PRESENTATION OF CASE We report a spontaneous resolution of large congenital parameatal cyst in an otherwise healthy neonate whose mother had a recent history of urinary tract infection. A spontaneous resolution of the cyst was reported at the age of one month. link2 DISCUSSION A small parameatal cysts have shown spontaneous resolution approximately 25% of the time. Treatment options should not include aspiration or marsupialization as many cases have shown recurrence. The role of antenatal infection in development of parameatal cyst not yet been determined. CONCLUSION A large parameatal cyst could resolve spontaneously, routine observation recommended for majority of cases. INTRODUCTION With the rising burden of obesity, bariatric surgery is becoming more common as a treatment option. Laparoscopic adjustable gastric banding (LAGB) is considered safe and effective and is a popular procedure in Australia. However there are recognised complications to be aware of such as band erosion which can lead to infection and abscess formation. PRESENTATION OF CASE A 59-year-old caucasian female presented with fevers, rigors and feeling generally unwell. She was previously fit and well with her only past medical history being LAGB 14 years prior. Clinically the patient was in septic shock and required intensive care admission for inotropic support. On investigations the CRP was 227 and abdominal computed tomography (CT) revealed a splenic abscess. Follow up upper gastrointestinal endoscopy diagnosed an eroded gastric band in the stomach. The patient proceeded to laparoscopy, a gastrotomy was performed and the band was removed. The splenic abscess was concurrently drained and the patient treated with an extended course of intravenous and oral antibiotics. DISCUSSION Band erosion is a rare but serious complications of LAGB surgery along with band slippage, pouch dilatation and abscess formation. Patients are often asymptomatic making early diagnosis difficult. Upper gastrointestinal endoscopy is used to locate the band and recommended treatment is band removal via laparoscopy or laparotomy. CONCLUSION Band erosion should be suspected in patients with a history of LAGB presenting with nonspecific symptoms such as abdominal pain or fevers. This case also highlights the importance of appropriate patient follow up post operatively and counselling of operative risks and long-term complications. INTRODUCTION Gemella sanguinis is an extremely rare cause of infectious endocarditis, with only 12 cases previously reported in the literature. Here we report the third known case of isolated mitral valve endocarditis secondary to G. sanguinis. PRESENTATION OF CASE A 53-year-old man with mitral valve prolapse and history of recent dental instrumentation presented with malaise, thigh and finger pain and new pansystolic murmur. He was diagnosed with severe mitral insufficiency due to infectious endocarditis secondary to G. sanguinis. He underwent mitral valve replacement and was treated with a long course of antibiotics. DISCUSSION G. sanguinis is a rare cause of infectious endocarditis with very few reported cases in the literature. In the majority of reported cases, a strategy of valve replacement along with prolonged antibiotic course results in good outcome for the patient. The use of synthetic packaging films causes serious environmental problems due to difficulty in recycling and poor biodegradability. Therefore, the present study aimed to develop natural biopolymer-based packaging films. As natural materials, chitosan (CS)-based films containing various concentrations (0.05 %, 0.1 %, and 0.15 %) of the hexahydro-β-acid/2-O-methyl-β-cyclodextrin (HBA/M-β-CD) inclusion complex were prepared and evaluated for structural, physicochemical, antioxidant, and antimicrobial properties. Results of morphological analysis and Fourier transform infrared spectroscopy (FT-IR) demonstrated good compatibility between CS and the HBA/M-β-CD complex and indicated that intermolecular hydrogen bonds were probably formed. Moisture content of the films decreased, whereas water solubility, swelling ratio, and water vapor permeability increased after the addition of HBA/M-β-CD. Optical test showed that addition of the inclusion complex improved the UV light barrier property. link3 The mechanical properties of the film were considerably increased after the incorporation of 0.1 % HBA/M-β-CD. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of HBA/M-β-CD-CS films was ten times higher than that of the control CS film. Furthermore, the incorporation of HBA/M-β-CD conferred the films with good antimicrobial activity against various foodborne pathogens. In summary, our results indicated that encapsulation with M-β-CD was an effective way of introducing HBA into CS film. This film can be used as an active packaging material for food preservation. Polydimethylsiloxane (PDMS) has been extensively used as a supporting material for studies of cell mechanobiology, cell micropatterning and microscale-cell analysis in microfluidic chips due to its numerous advantages, such as low cytotoxicity, ease of modification, inexpensive costs and biocompatibility. However, the innate hydrophobicity of PDMS often poses a problem for stable cell adhesion, seriously limiting its applicability for prolonged cell culture. UV exposure and protein coating are suboptimal solutions, while chemical surface functionalization is often associated with laborious procedures and producing environmental toxics. Plasma treatment can render a hydrophilic substrate by altering the surface chemistry, but such effect is often short-lived due to its tendency to hydrophobic recovery. Variation of physical properties of the substratum are known to influence cell behaviour. Nevertheless, the combination of varying PDMS substratum properties via basecuring agent ratio and plasma treatment to stabilize the long-term culture of bone marrow derived stromal cells (BMSCs) still remain poorly understood. In this study, we developed a protocol to maintain the hydrophilicity of the plasma-treated PDMS over a range of substratum properties. This study demonstrated that varying the substratum properties of PDMS can enhance the stability of BMSC culture for at least three weeks, while plasma treatment with or without additional collagen coating further enhanced such effect. The changes in the physical properties of PDMS have rendered difference in BMSCs adhesion, proliferation and in-vitro plasticity, thereby offering a simple and effective strategy for PDMS surface modification to enable long term cell analysis in PDMS-based culture platform.
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