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Liquid hot water (LHW) treatment can be used to disrupt the fiber structure of rice straw. This in vitro ruminal batch culture study investigated the effect of LHW treatment on feed degradation, methane (CH4) production, and microbial populations. Rice straw was treated by LHW, and in vitro ruminal fermentation was performed using an automatic system with 72 h of incubation. Scanning electron microscopy showed that LHW treatment disrupted the physical structure of rice straw. Liquid hot water treatment decreased neutral detergent fiber and hemicellulose contents of the rice straw and increased neutral detergent solubles, water-soluble carbohydrates, and arabinose contents. Liquid hot water treatment increased dry matter degradation and volatile fatty acid concentration and decreased the acetatepropionate ratio, CH4 production, hydrogen accumulation, neutral detergent fiber degradation, and populations of protozoa, fungi, and cellulolytic bacteria. In summary, LHW treatment disrupted the cellulose-hemicellulose-lignin structure matrix of rice straw, leading to increased substrate degradability and decreased CH4 production. Therefore, the LHW treatment is a potential strategy to improve the nutritive value of forage such as rice straw and decrease the CH4 emissions in ruminants. This study aimed to identify interactions between state of lactation (dry or early lactating) and immune responder group (low, medium, or high) for energy metabolism traits as well as metabolic and immunological traits in dairy cows. In early lactation, when the energy priority of cows shifts toward the mammary gland, the energy available to be partitioned toward the immune system may differ among individuals. The equilibrium between energy supply from feed, digestion, and body reserve mobilization and energy expenditure with milk, immune system, methane, and heat production is delicate in this stage. Seventeen Holstein cows entering their second to fifth lactation were kept under comparable feeding, housing, and management conditions and were studied from 14 ± 6 d before calving to 11 ± 3 d after calving. Feed intake, milk yield, body condition, blood metabolites, and cortisol as well as gaseous exchange in respiration chambers were measured. The latter was used to quantify methane emission and to calculate lower availability of digestible energy in early lactation and not be able to sustain the shift from immune cell activation to proliferation. If so, the selection of environmentally friendly low-methane emitters could promote phenotypes with a compromised immune response in the critical early lactation. The first objective of this study was to determine whether differences would occur among teat end preparation techniques with regard to potential contamination of milk samples collected for bacterial culture. The second objective was to determine whether differences would be detected in genus or species of bacteria isolated from samples collected using the various methods as well as from contaminated or uncontaminated samples. Mammary quarter foremilk samples were collected from lactating dairy cattle at the University of Missouri Foremost Research Dairy Farm (Columbia). Four different teat end preparation methods were used to compare contamination rates in milk samples. Sampling techniques used before milk collection included (1) no preparation, (2) pre-milking disinfection and single-use towel drying of teats only, (3) scrubbing of the teat end with alcohol only, and (4) pre-milking disinfection, single-use towel drying, and scrubbing of the teat end with alcohol. Milk was plated on Columbia blood agar. Culata confirm that scrubbing the teat end with alcohol after pre-milking disinfection with an iodine-based teat disinfectant and drying of the teat minimizes contamination of the milk sample. Many contemporary dairy cattle housing practices are at odds with societal perceptions of positive animal welfare. The public (i.e., those external to the dairy industry) typically emphasizes the importance of naturalness for dairy cattle, such as through provision of pasture, freedom of movement, and the ability to interact socially with conspecifics. Yet, in the United States, the majority of lactating dairy cattle are reportedly housed without any access to pasture, and almost 39% of dairy farms use tiestalls, which restrict movement and social interactions. In addition to being in conflict with public expectations, a lack of pasture access and restrictive housing systems are also in conflict with the animals' own motivations, which can adversely affect their welfare. For example, dairy cattle are highly motivated to access pasture and show a reduction in oral stereotypies when allowed on pasture after periods of tethering. Calves housed without social contact have cognitive deficits and exhibit increased can Dairy Science Association®. This is an open access article under the CC BY-NC-ND license (http//creativecommons.org/licenses/by-nc-nd/4.0/).The objective of this study was to compare the economic performance of 2 sward types [perennial ryegrass (PRG; Lolium perenne L.) sown with or without white clover (Trifolium repens L.)] grazed by 3 cow genotypes. Physical performance data were collected from a 4-yr systems experiment based at Clonakilty Agricultural College, Clonakilty, Co. Cork, Ireland. The experiment compared 2 sward types (PRG-only swards and PRG-white clover swards), with each sward type being grazed by cows from 3 genotypes [Holstein-Friesian (HF), Jersey × HF (JEX), and Norwegian Red × JEX (3-way)]. All systems were stocked at 2.75 cows/ha with fixed fertilizer applications and concentrate supplementation. The data supplied 6 production systems (2 sward types × 3 cow genotypes). The production systems were modeled using the Moorepark dairy systems model (stochastic budgetary simulation model) under 2 scenarios, one in which land area was fixed and one in which cow numbers were fixed. The analysis was completed across a range of milk prices, calf prices, and reseeding programs. The analysis showed that in the fixed-land scenario with a milk price of €0.29/L, adding white clover to PRG swards increased profitability by €305/ha. In the same fixed-land scenario, JEX cows were most profitable (€2,606/ha), followed by 3-way (€2,492/ha) and HF (€2,468/ha) cows. In the fixed-cow scenario, net profit per cow was €128 greater for PRG-white clover swards compared with PRG-only swards. In this scenario, JEX was the most profitable per cow (€877), followed by HF (€855) and 3-way (€831). The system that produced the highest net profit was JEX cows grazing PRG-white clover swards (€2,751/ha). Regardless of reseeding frequency or variations in calf value, JEX cows grazing PRG-white clover swards consistently produced the highest net profit per hectare. Veal calves are at a high risk of disease early in life, which can lead to poor growth. Research is needed to determine interventions that can reduce disease and promote the growth of veal calves. The aim of this study was to determine the effects of fatty acid supplementation and the provision of a dry teat on the incidence of bovine respiratory disease (BRD), navel inflammation, and diarrhea, as well as calf growth. Upon arrival to a commercial veal facility (d 0), 240 Holstein bull calves from 2 cohorts were randomly assigned to 4 treatments using a 2 × 2 factorial arrangement (n = 60/treatment) (1) milk replacer (MR) without fatty acid supplementation and without access to a dry teat (control), (2) MR with fatty acid supplementation (NeoTec5g, Provimi, Brookville, OH) and without access to a dry teat (FAS), (3) MR without fatty acid supplementation and with access to a dry teat (TT), or (4) MR with fatty acid supplementation and with access to a dry teat (FAS+TT). Calves were housed in individual pens froas a tendency for the interaction of FAS and TT to decrease ADG for wk 1 to 5 but not for wk 5 to 10 and wk 1 to 10. There was no effect of FAS, TT, or their interaction on the risk of BRD, diarrhea, or navel inflammation. We saw no effect of our interventions on calf health or growth. More research is needed to determine whether other factors, such as failure of passive transfer, poor ventilation, barren housing, and low milk allowance in the first few weeks after arrival, may have affected the efficacy of our interventions. Regional Research Project NC-2042 has a main objective to study calf and heifer nutrition. Within this objective, feeding the postweaned heifer is considered a major priority to improve the profitability and sustainability of US dairy farms. Through optimizing nutrient utilization by precision feeding, using alternative feeds, high-fiber diets, and feed additives, this research group has worked to enhance dairy heifer nutrition. Research has focused on precision feeding heifers and incorporating high- and low-fiber diets into this system of feeding. This is accomplished by meeting the nutrient needs of the heifer for a desired rate of growth while enhancing total-tract nutrient digestibility, reducing waste and improving profitability. High-fiber forages have been studied as a means of controlling ad libitum dry matter intakes and thus weight gain in heifers. These results provide producers with a means of feeding heifers while reducing costs. Similarly, utilizing alternative feedstuffs in heifer diets has also been a major research area for this group including comprehensive research on distillers co-products, and new protein sources such as camelina and carinata meals. Results indicated that these products can be satisfactorily incorporated into heifer diets. Studying feed additives has also been a function of the research group. Research with Ascophyllum nodosum and cinnamaldehyde indicated that calves find these additives unpalatable and that supplementing cinnamaldehyde to postweaned heifers showed no benefit. However, sodium butyrate and yeast supplementation proved to be beneficial in the growth and feed efficiency of heifers. Research from this group has an effect on heifer feeding, resulting in new information that can aid in the sustainability of dairy farms. This review will focus on the area of postweaned heifer nutrition. The all-on-4 implant concept has been used to overcome anatomic limitations and has been reported to have high success rates. A verification device is commonly used to transfer the position of the angled distal abutment accurately. This article describes a digital workflow for fabricating a verification device to position the angled distal abutment with a hexagon connection during computer-guided flapless surgery. STATEMENT OF PROBLEM Laser sintering has several processing parameters, typically under the control of dental laboratory technicians. Laser scan speed is an important parameter, which has a significant effect on manufacturing time but may also affect the adaptation of restorations. However, limited information is available regarding its impact. PURPOSE The purpose of this in vitro study was to evaluate the intaglio surface adaptation of laser-sintered cobalt-chromium single-crown frameworks sintered at laser scanning speeds of 1, 3, and 6 m/s. MATERIAL AND METHODS A master bronze metal die was prepared and scanned by using a laboratory scanner to fabricate the metal frameworks for 4 groups (n=10). In group C, the frameworks were fabricated by using the lost-wax method (control). In group L1, L3, and L6, the frameworks were fabricated by using direct metal laser melting (DMLM) at laser scanning speeds of 1, 3, and 6 m/s. After fabrication, 3 scanning data sets were used to evaluate the intaglio surface adaptation the master die, the intaglio surface of each metal framework, and each metal framework seated on the master die.
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