Notes

SARS-CoV-2 antibodies seroprevalence in pet dogs via France utilizing ELISA and an programmed developed blotting analysis.
Material of the giant pill-millipede genus Zephronia Gray, 1832 recently collected from Thailand contains three new species Zephroniaenghoffi sp. nov., Zephroniagolovatchi sp. nov., and Zephroniapanhai sp. nov. The first Zephronia species recorded for Thailand, Z.siamensis Hirst, 1907, is also redescribed based on new specimens collected both from the type locality in Chonburi Province and from neighboring areas. Morphological characters of all new species, Z.phrain Likhitrakarn & Golovatch, 2021, and Z.siamensis are illustrated, and a distribution map of the confirmed Zephronia species occurring in Thailand is also provided.An overview of the Estonian terrestrial isopod fauna is given, based on literature data and material collected from 1984 to 2021. The identified material consisted of 10915 specimens belonging to 14 species and collected from 172 localities throughout Estonia. In combination with previous data from the literature data, there are now reliable records of 16 species of woodlice from Estonia. Two species, viz. Platyarthrushoffmannseggii Brandt, 1833 and Hyloniscusriparius (C. Koch, 1838), are new for the fauna. The latter has probably colonised Estonia recently and range expansions have been reported elsewhere. The data on Philosciamuscorum (Scopoli, 1763) are dubious, and this species is currently excluded from the Estonian list.Two new species of Archidermaptera are described and figured from the Middle Jurassic Jiulonghsan Formation of Daohugou, Inner Mongolia, China. Aneurodermaoiodes gen. & sp. nov. is described in the family Protodiplatyidae and Sinopalaeodermataconcavum sp. nov. is established in the family Dermapteridae. Both new species share the typical characters of the extinct suborder Archidermaptera (e.g., pentamerous metatarsi, filiform and multimerous cerci, externalized ovipositor). Aneuroderma gen. nov. is compared with other genera of the Protodiplatyidae, while S.concavum sp. nov. allows us to emend the diagnosis of the genus Sinopalaeodermata. We briefly discuss the diversity of Archidermaptera and challenges to understanding relationships among this mid-Mesozoic diversity.A new species of the frog genus Rana sensu lato from Wuyi Mountain, Fujian Province, China is described. Molecular phylogenetic analyses clustered the new species into the R.johnsi group and indicated that it was genetically divergent from its closely related species. The new species could be distinguished from its congeners by a combination of the following characters body size medium, SVL 41.4-45.6 mm (42.9 ± 1.9 mm, n = 4) in adult males and 47.6-50.3 mm (n = 2) in adult females; adult male with a pair of internal subgular vocal sacs; lateroventral grooves present on tip of toes; webbing on fourth toes reaching the tip of toe; transverse skin ridges distinctly present on the dorsal surface of thigh and tibia, the number large (mean 26.5 ± 2.7, range 22-29, n = 6); breeding males possess creamy white nuptial pad with tiny velvety spines on the dorsal surface of the first finger, divided into three parts.Freshwater habitats, especially cold springs, are environments in which the risk of extinction faced by organisms remains high due to human activities. To conserve endangered species, it is important to describe and name them. Here, a new, endangered freshwater anisogammarid amphipod species, Jesogammarus (Jesogammarus) acalceolussp. nov., found in a spring in Aomori Prefecture, Japan, is described which is potentially the sole remaining habitat of this species. Both morphological and molecular phylogenetic results strongly support the nesting of the new species within Jesogammarus. Jesogammarus (J.) acalceolussp. nov. is the first species of genus Jesogammarus that was found to lack a calceolus, a sensory organ located on male antenna 2. Thus, the diagnostic criteria for this genus required amendment. A reconstruction of ancestral calceoli, based on a molecular phylogenetic tree, revealed that the common ancestor of Jesogammarus possessed calceoli, which were secondarily lost in J. (J.) acalceolussp. nov. Our results indicate that this new species, which is key to clarifying the evolution of the calceolus, is of high conservation significance.Misconceptions about energy conservation abound due to the gap between physics and secondary school chemistry. This paper surveys this difference and its relevance to the 1690s-2010s Leibnizian argument that mind-body interaction is impossible due to conservation laws. Justifications for energy conservation are partly empirical, such as Joule's paddle wheel experiment, and partly theoretical, such as Lagrange's statement in 1811 that energy is conserved if the potential energy does not depend on time. In 1918 Noether generalized results like Lagrange's and proved a converse symmetries imply conservation laws and vice versa. Conservation holds if and only if nature is uniform. The rise of field physics during the 1860s-1920s implied that energy is located in particular places and conservation is primordially local energy cannot disappear in Cambridge and reappear in Lincoln instantaneously or later; neither can it simply disappear in Cambridge or simply appear in Lincoln. A global conservation law can be inferred in some circumstances. Einstein's General Relativity, which stimulated Noether's work, is another source of difficulty for conservation laws. As is too rarely realized, the theory admits conserved quantities due to symmetries of the Lagrangian, like other theories. Indeed General Relativity has more symmetries and hence (at least formally) more conserved energies. An argument akin to Leibniz's finally gets some force. While the mathematics is too advanced for secondary school, the ideas that conservation is tied to uniformities of nature and that energy is in particular places, are accessible. Improved science teaching would serve the truth and enhance the social credibility of science.
Multiple sclerosis (MS) is an autoimmune disease leading to physical, emotional and cognitive disability. High body mass index (BMI) may impact cognitive function and brain volume in MS. Yet, there is paucity of evidence addressing the impact of BMI on cognitive function and brain volume in MS.

The purpose of this study was to examine the effects of BMI on normal appearing brain volume and cognitive function in patients with relapsing-remitting MS.

A secondary data analysis of the NIH CombiRx study was conducted. Multivariate regression and mixed model analyses were executed to analyze the effect of BMI on brain volume and cognitive function.

The mean baseline age of the 768 participants was 38.2(SD = 9.4)years. 73% were female and 88.8% were Caucasian. The mean BMI was 28.8kg/m
(SD = 6.7). The multivariate regression and mixed model analyses failed to show a clinical effect of BMI on brain volume and cognitive function.

BMI did not show an effect on cognitive function and brain volume among MS patients. Although there is increased interest in the effects of modifiable factors on the course of MS, the effects of BMI on brain volume and cognitive function are debatable and warrant further research.ClinicalTrials.gov NCT00211887.
BMI did not show an effect on cognitive function and brain volume among MS patients. Although there is increased interest in the effects of modifiable factors on the course of MS, the effects of BMI on brain volume and cognitive function are debatable and warrant further research.ClinicalTrials.gov NCT00211887.[This corrects the article on p. 116 in vol. 12, PMID 34526745.].Women are generally spared from abdominal aortic aneurysm (AAA) formation by the immunomodulating effects of estrogen. However, once they develop it, especially in the postmenopausal group, its behavior is more sinister with rapid expansion, a higher tendency to rupture, and higher mortality as compared to the male counterparts. Reported here is a case of AAA in a postmenopausal woman who came to the outpatient department with low backache, vague abdominal pain, and dysuria which initially gave a picture of pelvic inflammation with urinary tract infection but was later found out to be aortic aneurysm.Port site hernia (PSH) has been reported following both laparoscopic and robotic surgery. Subsequent surgical intervention increases postoperative morbidity. We report the case of a PSH through the 8-mm trocar following robot-assisted hysterectomy in a 49-year-old female patient, along with a review of the literature. The case was performed with the standard protocol; however, increased intraoperative bleeding was encountered from right uterine artery and vein. Discharged at 48 h, she presented in emergency on the fourth postoperative day with acute intestinal obstruction. Computed tomography scan showed herniation of the jejunal loop through the 8-mm left-sided port. She underwent resection and anastomosis of the necrosed jejunal loop. We review the literature for PSH following robotic gynecological surgeries. Although rare, PSH requires surgical intervention, increasing the postoperative morbidity. Need for fascial closure of 8 mm ports should be considered. High index of suspicion and early recognition can avoid resection of the bowel loop.Endocervical polyps are a common occurrence in the postmenopausal age group and many reports have described the presence of heterologous elements in the stroma of such polyps. The presence of mature adipose tissue in the stroma has been hypothesized to be arising from the perivascular fat or metaplastic transformation of the smooth muscle cells posttrauma. A 75-year-old female presented with spotting per vagina. Colposcopic examination revealed an endocervical polyp which was excised. Microscopic examination showed an ulcerated epithelium with metaplastic changes along with sheets of mature adipose tissue with focal S100 positivity conferring a diagnosis of choristomatous endocervical polyp with heterologous adipose tissue. The primary clinical concern of a malignant cause in this age group is alleviated by the diagnosis of this rare benign entity. Only three such cases have been reported in the English literature so far, and the present case accounts for the fourth such case.Malakoplakia is a chronic xanthogranulomatous condition that affects the genitourinary tract reported earlier as urinary granulomas and pelvic masses. We report a different clinical manifestation of malakoplakia presenting as postmenopausal pyometra. A 64-year-old postmenopausal female presented with foul-smelling vaginal discharge with a past history of induced abortion, followed by dilatation and evacuation. On examination, abdomen was soft, vaginal examination revealed pus discharge, parous size uterus with free fornices, and pap smear ruled out malignancy. Ultrasonography revealed linear, echogenic structures in the endometrial cavity suspicious of bony spicules with fluid around. Hysteroscopy revealed congested endometrium with multiple pieces of shredded bone-like structures that were removed followed by curettage. Histopathological examination was suggestive of malakoplakia with osseous metaplasia. Retained bony spicules can cause chronic granulomatous inflammation that may become symptomatic postmenopause due to absent cyclical shedding. This is the first reported case of malakoplakia of uterus following retained bony spicules.The risk of premalignant and malignant endometrial pathologies increases in the postmenopausal period. Dilatation and curettage fail to diagnose one in ten endometrial pathologies. Hysteroscopy is the gold standard to evaluate the endometrial cavity. Hysteroscopy can identify malignant or benign pathology with approximately 20% false-positive results. Hysteroscopy combined with biopsy increases the accuracy of diagnosis up to 100%. This pictorial review takes you through the hysteroscopic view of normal-looking postmenopausal atrophic uterus, cystic atrophy, benign endometrial pathologies, endometrial hyperplasia, endometrial cancer, tamoxifen-induced endometrial hyperplasia and histiocytic endometritis. The purpose of this pictorial review is to guide the operator in systematic evaluation of the endometrial cavity with special attention to the thickness of the endometrium, vascular architecture, location of the lesion and surface aberrations, which adds value to the diagnosis and management of endometrial pathologies.
Cardiovascular disease (CVD) is one of the leading causes of death among Indian women but not a focus of current strategies to improve women's health.

To assess 10-year CVD risk and estimate the prevalence of CVD risk factors among underprivileged women.

A cross-sectional study among women aged 40-79 years in an urban underprivileged area of Bangalore city, using nonlaboratory-based World Health Organization and the International Society of Hypertension risk prediction chart and INTERHEART list of CVD risk factors. Blood pressure, random blood sugar, height, weight, and waist circumference were measured. The Chi-square test was done for the association between CVD risk status and risk factors. Multiple logistic regression performed to calculate adjusted odds ratios (ORs).

Of 1027 women, 11% women had high risk of CVD (≥20% risk of fatal/nonfatal cardiovascular event within next 10 years). The prevalence of CVD risk factors was high with 20.2% diabetes, 53.7% hypertension, 77% overweight/obesity, and 9n and health education strategies. Policy-makers need to initiate efforts toward improving affordability of healthy diets for the urban underprivileged.
Typical chest pain symptoms are the cause that requires individuals to seek out medical care in Acute coronary syndrome(ACS). Evidence suggests, symptoms labelled as 'atypical 'is more common in women with ACS. The present study focuses on the need for the implementation of a gender specific approach in the current scenario by identifying gender based differences that exist in clinical presentations of the patients with ACS. Early identification of women's prodromal and acute symptoms of Myocardial Infarction is an important step in provision of appropriate treatment modality. Present study focus on need for implementation of gender-specific approach in current scenario by identifying gender based differences that exist in risk factors, clinical manifestations in patients presenting with MI.

Cross- sectional analytical study was conducted among 240 Participants (120 males and 120 females). Both men and women diagnosed with MI, who survived, stabilized after admission was included in the study. Consecutived need for focused assessment for women as they increasingly presents with atypical symptoms. The current study also supports the need of a gender specific approach to avoid delay in diagnosis and care of them.
Gender based differences in risk factors and clinical presentation in men and women with myocardial infarction had been a focus in researches that emphasized need for focused assessment for women as they increasingly presents with atypical symptoms. The current study also supports the need of a gender specific approach to avoid delay in diagnosis and care of them.
Protective role of estrogen in COVID-19 was speculated once the epidemiological studies reported increased susceptibility of estrogen-deficient population - males and postmenopausal females to severe disease category and involvement of angiotensin-converting enzyme 2 receptors and renin-angiotensin- aldosterone system in pathophysiology.

An open-label randomized controlled trial was planned to assess the efficacy of short-course oral estradiol in preventing the clinical progression to severe disease and reduce case-fatality rate and the hospital stay duration in estrogen-deficient postmenopausal women. The intervention group (
= 40) received 2 mg per day of estradiol valerate per orally for 7 days along with the standard care, while the control group (
= 40) received only the standard care.

A significant difference was observed in the rate of reverse transcriptase-polymerase chain reaction negativization in the intervention versus control group at day 5 and day 7 of admission (42.5% vs. 15%,
= 0.007; 72.5% versus 50%,
= -0.026). No significant difference was noted in the duration of hospitalization (
= 0.213). A significant decrease was noted in the mean values of inflammatory biomarkers - D-dimer, lactate dehydrogenase, and C-reactive protein on day 5 in the intervention group. Interleukin-6 also showed a declining trend on day 5 in the intervention group, while a rising trend was noted in the control arm. Only one case (2.5%) in the intervention group while seven in the control group (17.5%) progressed to the moderate category; however, the difference was not statistically significant (
= 0.057).

Oral estradiol in postmenopausal females can be a novel and efficient option for managing nonsevere COVID-19 infection.
Oral estradiol in postmenopausal females can be a novel and efficient option for managing nonsevere COVID-19 infection.
The aim of this study was to determine the diagnostic accuracy of a hysteroscopic scoring system in predicting endometrial cancer and endometrial hyperplasia with atypia.

This is a prospective study involving 95 peri and postmenopausal women with abnormal uterine bleeding who underwent hysteroscopic-guided endometrial biopsy. After the calculation of hysteroscopic score, biopsy was obtained and sent for histopathological examination. Hysteroscopic diagnosis of carcinoma endometrium was made when the total score was ≥16 and a score ≥7 supported a diagnosis of endometrial hyperplasia with atypia.

Out of the 95 women, 46 (48.4%) women had postmenopausal bleeding. The mean age of women was 50.4 ± 10.3 years. Eight women were diagnosed to have endometrial cancer and eight had endometrial hyperplasia with atypia on histopathological examination. Using a hysteroscopy score ≥16, the sensitivity and specificity were found to be 62.5% and 90.8%, respectively, for diagnosing endometrial cancer. Hysteroscopy score ≥9 was found to be a better cutoff for diagnosing endometrial cancer using Youden index. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for diagnosing endometrial cancer with score ≥9 was 100%, 67.8%, 22.2%, and 100%, respectively. The sensitivity, specificity, PPV, and NPV for diagnosing endometrial hyperplasia with atypia with score ≥7 was found to be 75%, 58.6%, 14.3%, and 96.2%, respectively.

The hysteroscopic scoring system has a good diagnostic performance when a cutoff score ≥9 is used in predicting endometrial cancer. However, the scoring system has lower diagnostic accuracy in predicting endometrial hyperplasia with atypia.
The hysteroscopic scoring system has a good diagnostic performance when a cutoff score ≥9 is used in predicting endometrial cancer. However, the scoring system has lower diagnostic accuracy in predicting endometrial hyperplasia with atypia.
There is a culture of silence around menopause in India, more so among rural women. This study was conducted to assess the prevalence of menopausal symptoms, factors associated with the severity of menopausal symptoms, and perceptions regarding menopause among rural perimenopausal women.

A cross-sectional study was conducted at a rural hospital, among women aged 40 years or more, having experienced at least one menstrual cycle in the past 1 year. Structured interview schedule was administered to capture perceptions of menopause. Symptoms of menopause and severity were assessed using the Menopause Rating Scale (MRS) and participants screened for depression using patient health questionnaire-9.

Among 200 women, the prevalence of symptoms of menopause was 70%. Commonly reported symptoms were somatic symptoms Joint/muscular pain, hot flushes, and psychological symptoms Physical/mental exhaustion, anxiety. Median overall MRS score was low (4 [interquartile range = 1, 7]), pointing out to lesser severity. Sig interventions using community women's groups and village-level health workers are recommended to provide not only awareness regarding menopause but also an opportunity to screen for comorbidities with appropriate referrals.
The objective is to compare endothelial dysfunction measured by brachial artery flow-mediated dilation (BAFMD) in nonobese, nondiabetic post-menopausal women with their age-matched menstruating controls and to identify the correlation of BAFMD with Framingham risk score (FRS) and with the individual parameters of FRS in low-risk women.

This study was done in the department of Obstetrics and Gynaecology, Chandigarh, India, for 1 year. Fifty postmenopausal and 50 menstruating females aged 45-55 years who were nondiabetic and nonobese and were low risk according to FRS were selected as cases and controls, respectively. All cases and controls were age-matched. The diameter of the brachial artery and the blood flow in it was measured at rest. Ischemia was produced and released after 5 min. The maximum blood flow velocity diameter of the brachial artery was measured. After 10 min of reactive hyperemia, 400 μg of sublingual nitrate was given, and vasodilatation mediated by nitroglycerine was subsequently measuretion, but it has a significant effect on vascular smooth muscle function. To know the effect of longer duration of menopause on vascular function in elderly women further studies with large number of postmenopausal women of different duration of menopause, may be needed.Obesity and overweight are associated with increased risks of cardiovascular and metabolic disease and overall poor health outcomes. Menopause is associated with significant changes in body composition and the accumulation of peri-abdominal or visceral fat. Changes in energy expenditure and spontaneous activity have been noted. These mid-life changes can add further to the burden of obesity and its associated risks. An understanding of the role of estrogen, gonadotrophins, gut hormones, sleep deprivation and the microbiome is still rudimentary, but research will ultimately provide further targets for more effective management. This narrative review will explore the pathogenesis of body composition changes at menopause, the impact on health outcomes and therapeutic and management options.[This retracts the article on p. 376 in vol. 11, PMID 33850405.].A 15-year-old female patient reported with a chief complaint of reduced facial esthetics due to proclination of incisors and excess visibility of teeth during smile. On clinical and radiographic examination, she had convex facial profile, Angle's Class I malocclusion, proclination of incisors with deep bite and excessive gingival display on smile. Comprehensive orthodontic therapy using fixed appliances, with four first premolar extraction and simultaneous intrusion and retraction of the anteriors with the help of midline mini-implant resulted in achieving all the treatment objectives without any adverse effects on the periodontium or the dentition.Gingival recession predisposes to functional and aesthetic concerns, for which many surgical procedures are available. Among these, till now subepithelial connective graft is considered as the gold standard technique for its higher predictability of achieving recession coverage. However, it does not heal by new attachment, thus many techniques to promote new attachment such as root biomodification are done. This case enumerates the use of Er, Cr YSGG laser for root biomodification to treat a wide and deep gingival recession defect by subpedicle connective tissue graft. Six months results show better recession coverage inspite of a wide and deeper defect. Thus, Er, Cr YSGG laser can be an adjunctive tool to enhance the recession coverage.A solitary median maxillary central incisor (SMMCI) is a rare anomaly that can occur alone or be associated with other systemic abnormalities. Early diagnosis of SMMCI is crucial as it might indicate the presence of an associated congenital or developmental abnormality. The prevalence of live-born children with SMMCI is determined to be 150,000 and is more common among females. The purpose of this paper was to report an unusual case of a 9-year-old girl with SMMCI who had no growth deficiency or any other systemic involvement. Since pediatricians and dentists are the first professionals to evaluate an SMMCI's patient in most cases, it is important that they be aware of the possibility of other related systemic problems that require systemic care. Appropriate treatment, diagnosis, and referral should also include neuropediatric evaluation, genetic testing, and craniofacial profile analysis along with multidisciplinary approach.The occurrence of the supernumerary tooth in the nasal cavity is uncommon. If not diagnosed and treated early, it may lead to various complications. Nasal endoscopy not only helps in proper diagnosis but also is an effective approach towards the extraction of the nasal tooth. It increases precision and reduces morbidity associated with the surgery.Although titanium is considered as the biocompatible material and widely used in medical and dental fields, the clinical application of this material is still a critical issue due to the possible adverse host response. Very few case reports related with titanium-based hypersensitivity reactions with dental implants proved the existence of titanium allergy. The present case report describes 56-year-old male showing allergic symptoms after 1 week of dental implant placement with no perioral or facial signs, but eczema was shown on the distant body parts, and the complete remission was attained after removing the dental implant.Resin cement and preheated restorative resin composite may be used for luting laminate veneers. The main advantage of resin composite is increased wear resistance, which could lead to better marginal performance in long term. This article reports a clinical treatment with feldspar laminate veneers luted to the maxillary teeth with preheated resin composite in a private practice. Case was finalized in May 2009 and followed by 10 years. Excellent clinical service and remarkable long-lasting marginal integrity were observed after 123 months. Scanning electron microscopy analysis showed no wear, gaps, or ditching at the margins. Restorative margins showed a smooth transition between ceramic and tooth with no signs of degradation. Preheated resin composite for luting ceramic laminate veneers may be considered an excellent clinical option.
Clonidine is a common additive to local anesthetics for various regional and local nerve blocks. However, its effectiveness in dentistry has not yet been fully explored. Thus, this study was performed to evaluate the quality of anesthesia, vasoconstrictive effects, hemodynamic response, and pain control using a solution of 2% lignocaine hydrochloride with clonidine hydrochloride in comparison with the standard solution of 2% lignocaine hydrochloride and adrenaline bitartrate for pterygomandibular nerve blocks.

A parallel arm, triple-blind randomized controlled study was conducted on 152 patients belonging to ASA-I (American Society of Anesthesiologists) category in the age group of 18-45 years, requiring surgical extraction of impacted mandibular third molars. The patients were divided equally into two groups randomly by computer-generated sequence; Group 1 2% lignocaine hydrochloride with 1 ml of clonidine hydrochloride (150 μg/ml) and Group 2 2% lignocaine hydrochloride with adrenaline bitartrate 1 80,0aline.
Clonidine as an additive to lignocaine has proved to have the onset of action, vasoconstrictive properties, and pain control, equivalent to adrenaline. However, with better stability of hemodynamic variables and prolonged duration of action of LA with clonidine, it can be considered as a better, safer, and more effective additive to lignocaine than adrenaline.
The aim of this study was to compare the efficacy of NovaMin
(SHY-NM) and casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) (tooth mousse plus
) on enamel remineralization using DIAGNOdent
and scanning electron microscope (SEM).

Eighty-six natural permanent maxillary first premolars were selected and randomly divided into two groups of 43 each, Group A (NovaMin
) and Group B (CPP-ACPF). All the samples were assessed using DIAGNOdent
(KaVo) at the baseline, after demineralization, and remineralization after 7 days. Two samples were randomly selected from each group after remineralization to evaluate the surface changes using SEM at × 1000 and × 2000.

The mean value of remineralization was highest for Group A NovaMin
(6.56 ± 0.93) compared to Group B, CPP-ACPF (tooth mousse plus
) (6.02 ± 1.09). The maximum demineralization to remineralization value within the groups showed that the mean values in Group B CPP-ACPF (7.02 ± 3.02) was higher than Group A NovaMin
(6.42 ± 2.21). The difference in remineralizing potential between the groups and demineralization to remineralization value in within-group comparison was not found to be statistically significant.

On comparing Group A NovaMin
and Group B CPP-ACPF, Group B CPP-ACPF showed a higher amount of remineralization than Group A NovaMin
. From the present study, it can be inferred that both the experimental groups have the potential for remineralization.
On comparing Group A NovaMin™ and Group B CPP-ACPF, Group B CPP-ACPF showed a higher amount of remineralization than Group A NovaMin™. From the present study, it can be inferred that both the experimental groups have the potential for remineralization.
Frequent monitoring of glucose is important in the management of diabetes. A noninvasive painless technique was used to detect glucose levels with the use of saliva as a diagnostic fluid.

The aim of our study was to correlate the blood glucose levels with stimulated and unstimulated salivary samples and also to assess the reliability of using salivary glucose in diagnosing and monitoring the blood glucose levels in gestational diabetic patients.

The study was conducted among 100 clinically healthy nondiabetic individuals and 99 individuals suffering from gestational diabetes mellitus (GDM).

Fasting blood glucose estimation and postprandial salivary glucose estimation were done in stimulated and unstimulated salivary samples using glucose oxidase/peroxidase method.

Data obtained were subjected to normality test, and
≤ 0.05 was considered to be statistically significant. The correlation between blood and salivary glucose levels was evaluated using Pearson's correlation test.

A positive correlation was obtained for stimulated and unstimulated salivary samples in fasting and postprandial conditions. Linear regression analysis and receiver operating characteristic curve were plotted, and the optimal cutoff value for unstimulated and stimulated salivary glucose under fasting conditions was 5.1 mg/dl and 5.4 mg/dl, respectively. The optimal cutoff value for unstimulated and stimulated salivary glucose was 8.8 mg/dl and 9.3 mg/dl, respectively, in postprandial conditions.

Saliva appears to be a reliable biofluid to assess the blood glucose levels and can definitely be a reliable alternative to blood glucose in GDM patients.
Saliva appears to be a reliable biofluid to assess the blood glucose levels and can definitely be a reliable alternative to blood glucose in GDM patients.
Platelets play an important role in inflammation and hemostasis. Periodontitis, a chronic inflammatory disease, is linked to an increase in platelet activation leading to increased risk for atherosclerosis and cardiovascular diseases.

The aim was to evaluate whether platelet indices (mean platelet volume [MPV], platelet distribution width [PDW], and plateletcrit [PCT]) can be a biomarker for determining the severity of periodontal disease and to assess the relation between platelet indices in patients with periodontitis and healthy controls.

The study included 3 groups - moderate periodontitis, severe periodontitis, and systemically healthy controls without periodontitis. Clinical parameters recorded were clinical attachment level and probing pocket depth and venous blood samples were drawn for the analysis of MPV, PDW, and PCT.

ANOVA test with
Tukey's test was used to compare among 3 groups. Statistical analysis of platelet indices was done using sample
-test. The mean values of MPV, PDW, and PCT gradually increased from normal to severe periodontitis.

Periodontitis, a chronic inflammatory process, causes not only increase in the quantity of platelets but also causes platelet activation which leads to change in platelet size, platelet shape, and platelet aggregation. As periodontitis causing platelet activation which seems to be a contributing factor in the development of cardiovascular diseases.
Periodontitis, a chronic inflammatory process, causes not only increase in the quantity of platelets but also causes platelet activation which leads to change in platelet size, platelet shape, and platelet aggregation. As periodontitis causing platelet activation which seems to be a contributing factor in the development of cardiovascular diseases.
Aerosols and droplets contaminated with bacteria and blood are produced during ultrasonic scaling. Measures to control aerosol contamination in dental clinics are recommended by the Centers for Disease Control and Prevention. This study aimed to evaluate the efficacy of preprocedural boric acid (BA) mouthrinse in reducing bacterial contamination in dental aerosols generated during ultrasonic scaling.

This was a randomised clinical trial in 90 systemically healthy subjects (25-55 yrs) diagnosed with chronic periodontitis who were allocated into three groups of 30 subjects each to receive, Group A - 0.12% chlorhexidine (CHX), Group B - 0.75% BA and Group C-water, as a preprocedural rinse for 1 min. The aerosol generated while performing ultrasonic scaling for 30 min was collected at three locations on blood agar plates. Incubation of the blood agar plates at 37°C for next 48 h for aerobic culture was performed and subsequently colony-forming units (CFUs) were counted.

CFUs in Group A were significantly reduced compared with Group B (
< 0.001). When we compare CFUs in all the three groups, CFUs in Groups A and B were statistically significantly reduced compared with Group C (
< 0.001). Furthermore, it was also observed that the assistant's chest area had lowest CFUs whereas patient's chest area had highest.

This study recommends routine use of preprocedural mouthrinse as a measure to reduce bacterial aerosols generated during ultrasonic scaling and that 0.12% CHX gluconate is more effective than 0.75% BA mouthwash in reducing CFUs count.
This study recommends routine use of preprocedural mouthrinse as a measure to reduce bacterial aerosols generated during ultrasonic scaling and that 0.12% CHX gluconate is more effective than 0.75% BA mouthwash in reducing CFUs count.
Sclerostin is an inhibitor of bone formation, and laser irradiation enhances osteoblast proliferation. The objective of this study was to assess and compare the gingival crevicular fluid (GCF) sclerostin level and clinical parameters of chronic periodontitis patients following the application of diode laser (810 nm) as an adjunct to scaling and root planing (SRP).

Fifteen systemically healthy chronic periodontitis patients (age 35-55 years) with probing pocket depth ≥5mm were included in this split-mouth study. SRP and pocket irradiation with diode laser were done in the test group and SRP alone in the control group at baseline. Low-level laser therapy application and saline irrigation were done in both the groups, respectively, in the 2
and 3
visits. Two microliters of GCF samples was collected from both the groups at baseline before treatment and on the 90
day for the assessment of sclerostin concentration.

This study showed a statistically significant reduction of clinical parameters in the test and control groups at the end of 3 months. Both the groups showed a statistically significant reduction of sclerostin levels in GCF after 3 months, in which the test group (125.80 ± 28.21 to 82.80 ± 9.31) showed a highly significant reduction (
= 0.000).

The adjunctive use of laser had shown a beneficial effect in terms of clinical parameters and osteoblast proliferation by the reduction in the levels of sclerostin in GCF. From the observations of this study, it can be concluded that the therapeutic effectiveness of diode laser as an adjunct to SRP is having a beneficial effect and sclerostin can be used as a potent biomarker.
The adjunctive use of laser had shown a beneficial effect in terms of clinical parameters and osteoblast proliferation by the reduction in the levels of sclerostin in GCF. From the observations of this study, it can be concluded that the therapeutic effectiveness of diode laser as an adjunct to SRP is having a beneficial effect and sclerostin can be used as a potent biomarker.
The aim of this study was to evaluate radiation exposure in dental open clinics in King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS) using thermoluminescence dosimeters (TLDs) to check if it exceeds the annual assigned exposure limit and to assess students' practices regarding radiation protection measures and their knowledge regarding the application of digital remote-control settings and TLDs.

This pilot institutional-based observational study was conducted among the clinical year students and interns at KSAU-HS College of Dentistry open clinical areas using TLDs and questionnaires. Sixteen lithium fluoride TLDs (TLD-100) were distributed evenly in the clinical areas occupied by clinical year dental students and interns for 24 working days from September 15 to October 20, 2019. Each TLD was labeled with a serial number and fixed at the assigned clinic of each specialty. The TLDs were placed in a zigzag manner at the right corner of the selected clinic to prevent overlapping of the area cosure from dental imaging and the safety of intraoral radiographic machines present in open clinics in any educational institute necessitates conducting this kind of study.
Welded diamond and vacuum diffusion technology (WDVDT) burs in comparison to electroplated burs claim to approach the solution of dental hard tissues by increased cutting efficiency, decreasing the overheating of oral tissues and thus reducing the microcracks on the prepared tooth surface.

This study aimed to evaluate the cutting efficiency of two different rotary diamond burs used for tooth preparation with their repeated usage on the surface changes of the prepared tooth.

This
comparative study evaluated the cutting efficiency and surface changes of the teeth prepared with conventional electroplated burs and WDVDT burs.

Thirty freshly extracted, healthy, noncarious human premolars were divided into Group A and Group B with 15 each, and were further subdivided into three subgroups depending on the different usage intervals as first, fifth, and tenth (A1-A3 and B1-B3). The surface of each prepared specimen was evaluated quantitatively using a surface profilometer, and qualitative analysis was done using a scanning electron microscope.

Two-way ANOVA and Turkey's multiple
tests were used.

The mean surface roughness of Groups A1, A2, and A3 was 1.50 ± 0.40, 2.39 ± 0.39, and 2.65 ± 0.65 Ra, respectively. The mean surface roughness of Groups B1, B2, and B3 was 0.76 ± 0.23, 0.92 ± 0.10, and 1.24 ± 0.07 Ra, respectively. The mean surface roughness of the prepared tooth surface was significantly higher in Group A and its subgroups when compared to that of Group B and its subgroups.

The study results showed that surface roughness was considerably lesser and also had less wear and increased cutting efficiency of tooth preparations using burs made with WDVDT compared to the preparations using conventional electroplated burs.
The study results showed that surface roughness was considerably lesser and also had less wear and increased cutting efficiency of tooth preparations using burs made with WDVDT compared to the preparations using conventional electroplated burs.
Empathy is one of the fundamentals of communication relevant within the practice of dentistry, and the research is limited in this field.

The present study was conducted to assess the self-reported empathy levels among dental undergraduate students in Lucknow city of Northern India.

A descriptive cross-sectional study was conducted among three dental colleges of Lucknow city during July 2019-September 2019.

E-survey link was circulated among 790 students through e-mails. It has two parts. The first part included sociodemographic information, wherein the second part, the empathy level of students was assessed using the Jefferson Scale of Physician Empathy-Health Profession Students Version Questionnaire. The possible score range is 20-140 the higher the mean score, the higher the self-reported empathy level.

The data collected were analyzed using SPSS software version 24. Chi-square test was used to test gender distribution differences among different years. One-way analysis of variance including
tests was used to compare the differences between study variables.

The mean ± standard deviation empathy score was 92.55 ± 11.85, 91.85 ± 12.23, 93.25 ± 11.24, 93.37 ± 12.31, and 88.34 ± 12.01 among the first-year, second-year, third-year, final-year, and interns' students, respectively, with a statistically significant difference.

It can be concluded from the results of the present study that the mean empathy score was highest among final-year students which is suggestive of the impact of education in behavioral sciences.
It can be concluded from the results of the present study that the mean empathy score was highest among final-year students which is suggestive of the impact of education in behavioral sciences.
Cancer stem cells (CSCs) belong to a subpopulation of undifferentiated cells present within tumors that have the potential to regenerate, differentiate, maintenance of pluripotency, drug resistance, and tumorigenicity when transplanted into an innate host. These can influence the growth and behavior of these tumors and are used to investigate the initiation, progression, and treatment strategies of laryngeal cancer. Research on CSC science and targeted therapies were hinge on their isolation and/or enrichment procedures. The object of the study is to isolate cancer stem cells from primary laryngeal carcinoma (CSCPLC) by tumor spheres enrichment. We checked the properties of self-renewal, stemness, clonogenicity, and chemotherapeutic resistance.

We performed tumor sphere formation assay (primary, secondary, and tertiary) chemotherapy resistance by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay were performed to evaluate the CSC cells. Immunofluorescence for stem cell markers (CD133+, CDtion of CSC from PLC cell lines. These cells demonstrated the properties of self-renewal, stemness, clonogenicity, and chemotherapeutic resistance.
Microleakage is the major cause for the failure of dental restorations, especially in Class V cavities, as margins of such restorations are generally located in dentin or cementum. Microleakage evaluation is necessary as a means of evaluation of the marginal integrity of restorative materials. This would assist in developing techniques and materials that would reduce damage caused by the failure of the restorative marginal seal.

The aim of this study is to analyze and compare the marginal integrity among three esthetic restorative materials, namely GC Fuji II LC, GC G-Aenial anterior composite resin, and GC Equia forte fil.

Sixty orthodontically extracted caries-free premolar teeth with Class V restorations were divided into three groups. Microleakage was measured using an ordinal scale of 0-4, as given by Khera and Chan, in increasing order of dye penetration, which was observed under a microscope.

Study was conducted in sound human extracted premolars in which Standardized Class V cavities were prepleakage at the tooth restoration interface. It was evident that microleakage was found to be highest with the Fuji II LC, both at occlusal and cervical levels. GC Equia forte exhibited the best performance in limiting microleakage around the restoration margins.
Numerous studies have assessed the effect of photodynamic therapy (PDT) both as a primary mode of treatment and as an adjunct to scaling and root planing in the treatment of periodontitis. Some results were nondefinitive and, in part, inconsistent with respect to the clinical and biochemical effects. Hence, the aim of this study was to evaluate the effect of PDT as an adjunct to nonsurgical periodontal therapy (NSPT) on the gingival crevicular fluid (GCF) interleukin-6 (IL-6), IL-8, and IL-10 levels in the treatment of chronic periodontitis (CP).

In 21 patients with CP, two contralateral sites (premolar and molar) were randomly divided into control sites (treated with NSPT only) and test sites (treated with NSPT + PDT). Clinical parameters including bleeding on probing (BOP), probing pocket depth, clinical attachment level were evaluated at baseline, 1- and 3 months and biochemical parameters of GCF levels of IL-6, IL-8, and IL-10 were evaluated at baseline and 3-month post-therapy through enzyme-linked immunosorbant assay.

A greater improvement in BOP score at 1 month (41.10% ± 3.58%) and 3-months (38.00% ± 3.62%) posttherapy was found in the test site as compared to control site. Regarding cytokines, test sites exhibited significant reductions in IL-6 (4.29 ± 0.67 pg/ml) and IL-8 (308.16 ± 36.04 pg/ml) levels and increase in IL-10 (14.25 ± 0.83 pg/ml) level at 3 months (
< 0.0001).

Additional application of PDT, adjunctive to NSPT, resulted in a significant reduction in BOP score as well as GCF pro-inflammatory cytokine levels along with an increase in anti-inflammatory cytokine levels, compared to NSPT alone.
Additional application of PDT, adjunctive to NSPT, resulted in a significant reduction in BOP score as well as GCF pro-inflammatory cytokine levels along with an increase in anti-inflammatory cytokine levels, compared to NSPT alone.
Biomechanical preparation of root canal can damage root dentin leading to the formation of dentinal cracks which can eventually lead to fracture and failure of the treatment.

The aim was to investigate the incidence of dentinal defects in root canals prepared with ProTaper Universal, ProTaper Gold, and Hyflex electric discharge machining (EDM) rotary file systems using handheld USB digital microscope.

One hundred and fifty extracted mandibular premolar teeth with single canal were randomly divided into five groups (
= 30). Group 1 unprepared (negative control). Group 2 canal preparation done with nickel-titanium hand files (positive control), Group 3 canals prepared with ProTaper Universal rotary system, Group 4 canal preparation done with ProTaper Gold rotary system, and Group 5 canals prepared with Hyflex EDM rotary system. The specimens were sliced at 3 mm, 6 mm, and 9 mm from the apex with a slow-speed saw under water cooling. Digital images of each section were captured at ×40 magnification with the aid of a microscope. Two independent evaluators assessed the images for the presence of dentinal defects. The number of dentinal defects was recorded and Chi-square test was used for statistical analysis (
< 0.05).

The number of specimens presenting dentinal defects was as follows Group 3; ProTaper Universal - 9/30 (30.0%), Group 4; ProTaper Gold - 2/30 (6.7%), and Group 5; Hyflex EDM - 1/30 (3.3%). No defects were detected in the negative and positive control groups (Group 1 and Group 2).

ProTaper Universal showed the highest percentage of defects in comparison to ProTaper Gold and Hyflex EDM.
ProTaper Universal showed the highest percentage of defects in comparison to ProTaper Gold and Hyflex EDM.
Irrigants were required to eliminate the microbes and debris from the intraradicular space and must have direct contact with the entire root canal wall. Therefore, different irrigation methods have been proposed to deliver the irrigant as close as possible to the remote areas of the root canal.

The aim of the present study is to evaluate the cleaning efficacy of single-beveled needle, side-vented needle, endovac, and endo-irrigator plus in the removal of debris from apical third of root canal by Scanning Electron Microscope.

Forty single-rooted freshly extracted human permanent mandibular premolars were collected. Root canals were cleaned and instrumented till X2 (25/06) with rotary Protaper Next at working length 1 mm short of the apex. Teeth were randomly divided into four equal groups Group 1 (
= 10) Endovac, Group 2 (
= 10) Endo irrigator plus, Group 3 (
= 10) Side-vented needle, and Group 4 (
= 10) Single-beveled needle. Irrigation was done with 5.25% NaOCl, followed by 17% ethylenediaminetetraacetic acid. Samples were sectioned and examined under SEM at apical levels.

Analysis of variance followed by Tukey's
test was performed.

The level of debris removal efficacy is as follows Endovac > Endo-irrigator plus > Side-vented needle ~ Single-beveled needle.

Endovac showed the maximum number of debris removal and has better cleaning efficacy in the apical areas of the root canal, followed by Endo irrigator plus, Side-vented needle and Single-beveled needle.
Endovac showed the maximum number of debris removal and has better cleaning efficacy in the apical areas of the root canal, followed by Endo irrigator plus, Side-vented needle and Single-beveled needle.
The oral squamous cell carcinoma (OSCC) often affects elderly aged adults, who had long-term tobacco exposure. In the recent past decade, there is an increasing incidence of OSCC in younger individuals, who had less tobacco association.

The aim of this study is to examine the risk factors and to assess and compare the clinicopathological features and prognosis of OSCC in young patients with the older group patients.

We included studies that addressed the OSCC involving young patients during the period between 2014 and 2019. Initially, we found 679 articles; after refined evaluation, 92 works had their titles matching to the present work. After extraction of case reports, reviews, and studies with un-matched objectives or inadequate data, we have chosen only 24 presentations matching to the abstract. The statistical analysis performed by Chi-square tests using SPSS 20.0 version for windows.

Of the total OSCC, 10% of patients belonged to the younger age group, and 90% were the older aged group. Both the groups showed male gender predominance and tongue as the predominant site of involvement. The younger patients tend to have significantly reduced tobacco exposure. The highly reported tumors were advanced staged and moderately differentiated tumors in both groups. There was a statistically evident high recurrence reported in the younger group. The overall survival seems to be similar for both groups.

The identification of the potential risk factors, along with unique molecular mechanisms, and biological behavior of the disease is mandatory to control the incidence of OSCC in young adults.
The identification of the potential risk factors, along with unique molecular mechanisms, and biological behavior of the disease is mandatory to control the incidence of OSCC in young adults.
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