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Checking out the actual microbiota involving fermented seafood products (Pla-ra) from different towns associated with northeastern Thailand.
Hydrogenotrophic methanogens were not affected by any concentration, indicating their lower sensitivity to these compounds when compared to acetoclasts and acetogens.Daphnia has been widely used as an indicator species in aquatic biomonitoring for decades. Traditional toxicity assays based on lethality take a long time to assess, and the effect mode of contaminants is not clear. Because of the translucency of the Daphnia body and the application of fluorescent probes in cell staining, different intoxicated parts can be visualized. In this study, a double-staining method using two fluorescent dyes, Calcein AM (cell-permeant dye) and Propidium Iodide (cell-impermeant dye), was carried out on Daphnia magna exposed to six pathogens Salmonella spp. (four strains) and Shigella spp. (two strains). The results showed that those bacteria caused different infections on daphnia depending on the age of this organism and bacterial concentrations. In detail, S. dublin and S. sonnei are the most harmful to Daphnia when they cause damage at smaller concentrations at the younger stage (3 weeks old). Interestingly, older Daphnia can give responses to nearly 10 CFU/ml to less than 100 CFU/ml of some bacteria strains. In another experiment, S. sonnei disturbed Daphnia after just 10 min of exposure, and Daphnia adapted to S. choleraesuis, S. typhi, and S. flexneri at the early stage (3 weeks old) after 1 h of exposure. Moreover, the damaged areas of the daphnia body were directly observed via a microscope, contributing to the understanding and the prediction of toxicity mechanisms.The dose limit for the skin of the hand is typically converted to a surface of 1 cm2, which means that one needs to measure point doses in different places on the hand. However, the commonly used method of measuring doses on the hand, i.e., using a dosimetric ring including one or several thermoluminescent detectors worn at the base of a finger, is not adequate for manual procedures such as labeling or radiopharmaceutical injection. Consequently, the purpose of this study was to create and conduct a series of computer simulations that, by recreating the actual working conditions, would provide information on the values of ionizing radiation doses received by the most exposed parts of the hands of employees of radiopharmaceutical production facilities, as well as those of nurses during the injection of radiopharmaceuticals. The simulations were carried out using Monte Carlo radiation transport calculations. The Hp(0.07) personal dose equivalent values obtained for the fingertips of the index and middle fingers of nursing staff and chemists were within the range limited by the minimum and maximum Hp(0.07) values obtained as a result of dosimetric measurements carried out in diagnostic and production centers. Only in the case of the nurse's fingertip, the simulated value of Hp(0.07 slightly exceeded the measured maximum Hp(0.07) value. The comparison of measured and simulated dose values showed that the largest differences in Hp(0.07) values occurred at the thumb tip, and for ring finger and middle finger of some of the nurses investigated.
To compare CT texture features of benign and malignant ovarian lesions and to build a machine learning model to detect malignancy in incidental ovarian lesions.

In this IRB-approved, HIPAA-compliant, retrospective study, 427 consecutive patients with incidental ovarian lesions detected on contrast-enhanced CT (348, 81.5% benign and 79, 18.5% malignant) were included. The following CT texture features were analyzed using commercially available software (TexRAD, Feedback Plc, Cambridge, UK) total pixel, mean, standard deviation (SD), entropy, mean value of positive pixels (MPP), skewness, kurtosis and entropy. Three machine learning models were created by combining texture features and patients' age, and performance of these models was assessed using tenfold cross-validation. Receiver operating characteristics (ROC) were constructed to assess sensitivity and specificity. The cutoff value was picked using a cost-weighted method.

Total pixels, mean, SD, entropy, MPP, and skewness were significantly different between benign and malignant groups (p < 0.05). With a selected 10 as a cost factor to optimize cutoff value selection, sensitivity 92%, specificity 60% in the random forest (RF) model, sensitivity 91%, specificity 69% in SVM model, and sensitivity 92%, specificity 61% in the logistic regression, respectively.

CT texture analysis could provide objective imaging analysis of incidental ovarian lesions and ML models using CT texture features and age demonstrated high sensitivity and moderate specificity for detection of malignant lesions.
CT texture analysis could provide objective imaging analysis of incidental ovarian lesions and ML models using CT texture features and age demonstrated high sensitivity and moderate specificity for detection of malignant lesions.Despite being grouped together in epidemiological risk categories, gay, bisexual, and other men who have sex with men (GBM) are not a homogenous group. In addition to traditional segmentation along race, ethnicity, and socioeconomic status, many GBM also identify with sexual subcultural communities. Previous research has shown differences across a variety of health outcomes between these sexual subcultural communities. The purpose of this study was to determine whether HIV prevention practices among GBM differed according to sexual subcultural community. The study was conducted in collaboration with a popular social and sexual networking smartphone application company. A total of 23,577 GBM responded to the survey. A latent class analysis identified 6 distinct classes related to sexual subcultural community identification. We found significant differences across sociodemographic characteristics, HIV prevention practices, and condomless anal sex in the past 6 months related to sexual subculture identification. Findings suggest that sexual subcultural identity is related to decision-making around HIV prevention among GBM. Differences in HIV prevention strategies are likely a function of group norms, unique shared experiences among GBM identifying with a particular sexual subculture community, and sociodemographic characteristics associated with these groups. As such, sexual subculture identity should be considered in developing interventions and social marketing campaigns to increase uptake of biomedical HIV prevention tools among GBM. Identifying group norms and shared experiences related to HIV prevention practices among sexual subcultures is necessary to understand the role these identities play in lives of GBM, especially as it relates to their sexual health and well-being.The present research explored fantasies about consensual nonmonogamous relationships (CNMRs) and the factors that predict such fantasies in a large and diverse online sample (N = 822) of persons currently involved in monogamous relationships. Nearly one-third (32.6%) of participants reported that being in some type of sexually open relationship was part of their favorite sexual fantasy of all time, of whom most (80.0%) said that they want to act on this fantasy in the future. Those who had shared and/or acted on CNMR fantasies previously generally reported positive outcomes (i.e., meeting or exceeding their expectations and improving their relationships). In addition, a majority of participants reported having fantasized about being in a CNMR at least once before, with open relationships being the most popular variety. Those who identified as male or non-binary reported more CNMR fantasies than those who identified as female. CNMR fantasies were also more common among persons who identified as anything other than heterosexual and among older adults. Erotophilia and sociosexual orientation were uniquely and positively associated with CNMR fantasies of all types; however, other individual difference factors (e.g., Big Five personality traits, attachment style) had less consistent associations. Unique predictors of infidelity fantasies differed from CNMR fantasies, suggesting that they are propelled by different psychological factors. Overall, these results suggest that CNMRs are a popular fantasy and desire among persons in monogamous romantic relationships. Clinical implications and implications for sexual fantasy research more broadly are discussed.
Persistent postpartum voiding dysfunction (P-PPVD) is defined as the inability to empty the bladder properly 72h after delivery despite the use of intermittent urinary catheterization. Our aim was to find predictive factors for P-PPVD and to compare its urogynecologic outcomes with transient dysfunctions.

A case-control study was performed in a university hospital center between January 2018 and April 2019. The case group included women diagnosed with P-PPVD after vaginal delivery, and the control group included women with PPVD that resolved before 72h. Patients were followed up at 12weeks and 12months postpartum, including an ultrasound assessment of the levator ani muscle (LAM).

Of 2308 deliveries, 1894 (81%) were vaginal, 75 (3.85%) presented PPVD, and 1 lasted > 72h (0.69% P-PPVD). LAM avulsion (OR 6.3, 95% CI 1.24-32.01) was the only independent risk factor for P-PPVD found. No significant differences in urogynecologic symptoms between transient and persistent PPVD were found in the short and the long term, except that women with P-PPVD had a lower prevalence of urinary incontinence at 12weeks postpartum.

PPVD is a common, self-limited event, but in 17.3% of cases persists > 3days. Levator ani muscle avulsion acts as an independent risk factor for P-PPVD. Early diagnosis and appropriate treatment of P-PPVD can help minimize any clinical implications for long-term urogynecologic disorders.
3 days. Levator ani muscle avulsion acts as an independent risk factor for P-PPVD. Early diagnosis and appropriate treatment of P-PPVD can help minimize any clinical implications for long-term urogynecologic disorders.The efficacy of cellular immunotherapy plus chemotherapy in treatment of gastric cancer (GC) remains inconsistent even controversial. Hence, we performed a meta-analysis to better comprehend the clinical value of cellular immunotherapy plus chemotherapy for GC patients. We searched PubMed, Embase and EBSCO databases to identify the studies evaluating the association of cellular immunotherapy plus chemotherapy and overall survival (OS) and/or disease-free survival (DFS) in patients with GC, and then combined relevant data into hazard ratios (HRs) for OS, DFS and clinicopathological features such as TNM stage, etc. with STATA 12.0. Eleven studies with 1244 patients were included in this meta-analysis. We found that cellular immunotherapy plus chemotherapy remarkably improved overall survival (OS) and diseases-free survival (DFS) as compared to the chemotherapy for GC patients. In subgroup analyses, pooled data showed that the combined therapy was significantly associated with better 3-year and 5-year survival rate, but not with 1-year survival rate of patients; the application of cellular immunotherapy based on either CIK or DC-CIK cells could enhance survival as well as NK, γδT and CIK cells-based immunotherapy. More importantly, the addition of cellular immunotherapy considerably improved OS and DFS only in patients with stage III rather than stage II. In addition, we also discovered that the combined therapy did not cause intolerable side effects to patients. Cellular immunotherapy plus chemotherapy ameliorates survival in GC, especially in patients with stage III, implicating that it is a valuable therapeutic strategy for these patients.
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