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24-1.57, p less then 0.001] for hsTnI, 1.41 [95% CI 1.24-1.60, p less then 0.001] for hsCRP, and 1.64 [95% CI 1.39-1.92, p less then 0.001] for NT-proBNP. However, upon further adjustments for the other two biomarkers, only NT-proBNP was still associated with the combined endpoint with an HR of 1.47 [95% CI 1.19-1.82, p less then 0.001]. Conclusively, NT-proBNP is reliably linked to CV death and MI in patients with CAD and provides incremental value beyond hsCRP and hsTnI.Optimal patient selection for cardiac resynchronization therapy is crucial. There are several concerns that allow to better clarify the association between the ischemic etiology of heart failure and the response to cardiac resynchronization therapy. The type of ischemic coronary disease has an impact on the responses to cardiac resynchronization therapy. The prognostic impact of cardiac resynchronization therapy on cardiac death including heart transplantation and durable ventricular assist device implantation is another concern.Because of the increased demand for preceramic polymers in high-tech applications, there has been growing interest in the synthesis of preceramic polymers, including polysiloxanes and alumina. These polymers are preferred because of their low thermal expansion, conformability to surfaces over large areas, and flexibility. The primary objective was to evaluate the aspects of polymer-derived ceramic routs, focusing on the UV lithography process of preceramic polymers and the pyrolyzing properties of the final ceramics. We found that the p(DMS-co-AMS) copolymer was effective in scattering the hydrophilic Al2O3 nanoparticles into the exceedingly hydrophobic solvent. The physico-chemical behavior of characterized copolymers was explored during their pyrolytic transformation into amorphous silicon-based ceramics. The results indicate that an increase of the pyrolysis temperature degraded the Si-O network through the carbothermic reaction of silicon. We also found a rapid elimination of copolymer pores and densification when the temperature increased (1100 to 1200 °C). At different but specific temperature ranges, there are different distinct rearrangement reactions in the conversion of polymer to ceramic; reductions of the melting point (Tm) of the total heat of melting (ΔHm) of the pyrolysis process resulted in the crystallization of ceramic materials; hence, lithography based on pyrolysis properties of preceramic polymers is a critical method in the conversation of polymers.In this study, nine specimens were experimentally tested to analyze the strengthening efficiency of textile-reinforced mortar (TRM) and the difference in flexural behavior between prestressed and non-prestressed TRM-strengthened reinforced concrete beam. The test results show that TRM strengthening improves the flexural strength of TRM-strengthened reinforced concrete beams with alkali-resistant-(AR-) glass textile as well as that with carbon textile. However, in the case of textile prestressing, the strengthening efficiency for flexural strength of the AR-glass textile was higher than that of the carbon textile. The flexural stiffness of AR-glass textiles increased when prestressing was introduced and the use of carbon textiles can be advantageous to reduce the decreasing ratio of flexural stiffness as the load increased. In the failure mode, textile prestressing prevents the damage of textiles effectively owing to the crack and induces the debonding of the TRM.The development of a sensor based on molecularly imprinted polymer nanoparticles (nanoMIPs) and electrochemical impedance spectroscopy (EIS) for the detection of trace levels of cocaine is described in this paper. NanoMIPs for cocaine detection, synthesized using a solid phase, were applied as the sensing element. The nanoMIPs were first characterized by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering and found to be ~148.35 ± 24.69 nm in size, using TEM. The nanoMIPs were then covalently attached to gold screen-printed electrodes and a cocaine direct binding assay was developed and optimized, using EIS as the sensing principle. EIS was recorded at a potential of 0.12 V over the frequency range from 0.1 Hz to 50 kHz, with a modulation voltage of 10 mV. The nanoMIPs sensor was able to detect cocaine in a linear range between 100 pg mL-1 and 50 ng mL-1 (R2 = 0.984; p-value = 0.00001) and with a limit of detection of 0.24 ng mL-1 (0.70 nM). The sensor showed no cross-reactivity toward morphine and a negligible response toward levamisole after optimizing the sensor surface blocking and assay conditions. The developed sensor has the potential to offer a highly sensitive, portable and cost-effective method for cocaine detection.Speech production not only relies on spoken (overt speech) but also on silent output (inner speech). Little is known about whether inner and overt speech are processed differently and which neural mechanisms are involved. By simultaneously applying electroencephalography (EEG) and functional near-infrared spectroscopy (fNIRS), we tried to disentangle executive control from motor and linguistic processes. A preparation phase was introduced additionally to the examination of overt and inner speech directly during naming (i.e., speech execution). Participants completed a picture-naming paradigm in which the pure preparation phase of a subsequent speech production and the actual speech execution phase could be differentiated. fNIRS results revealed a larger activation for overt rather than inner speech at bilateral prefrontal to parietal regions during the preparation and at bilateral temporal regions during the execution phase. EEG results showed a larger negativity for inner compared to overt speech between 200 and 500 ms during the preparation phase and between 300 and 500 ms during the execution phase. Findings of the preparation phase indicated that differences between inner and overt speech are not exclusively driven by specific linguistic and motor processes but also impacted by inhibitory mechanisms. Results of the execution phase suggest that inhibitory processes operate during phonological code retrieval and encoding.A loop-mediated isothermal amplification (LAMP) assay was developed for simple, rapid and efficient detection of Cucurbit leaf crumple virus (CuLCrV), one of the most important begomoviruses that infects cucurbits worldwide. A set of six specific primers targeting a total 240 nt sequence regions in the DNA A of CuLCrV were designed and synthesized for detection of CuLCrV from infected leaf tissues using real-time LAMP amplification with the Genie® III system, which was further confirmed by gel electrophoresis and SYBR™ Green I DNA staining for visual observation. The optimum reaction temperature and time were determined, and no cross-reactivity was seen with other begomoviruses. The LAMP assay could amplify CuLCrV from a mixed virus assay. The sensitivity assay demonstrated that the LAMP reaction was more sensitive than conventional PCR, but less sensitive than qPCR. However, it was simpler and faster than the other assays evaluated. The LAMP assay also amplified CuLCrV-infected symptomatic and asymptomatic samples more efficiently than PCR. Successful LAMP amplification was observed in mixed virus-infected field samples. This simple, rapid, and sensitive method has the capacity to detect CuLCrV in samples collected in the field and is therefore suitable for early detection of the disease to reduce the risk of epidemics.Recent efforts to comprehensively characterize great ape genetic diversity using short-read sequencing and single-nucleotide variants have led to important discoveries related to selection within species, demographic history, and lineage-specific traits. Structural variants (SVs), including deletions and inversions, comprise a larger proportion of genetic differences between and within species, making them an important yet understudied source of trait divergence. Here, we used a combination of long-read and -range sequencing approaches to characterize the structural variant landscape of two additional Pan troglodytes verus individuals, one of whom carries 13% admixture from Pan troglodytes troglodytes. We performed optical mapping of both individuals followed by nanopore sequencing of one individual. Filtering for larger variants (>10 kbp) and combined with genotyping of SVs using short-read data from the Great Ape Genome Project, we identified 425 deletions and 59 inversions, of which 88 and 36, respectively, were novel. Compared with gene expression in humans, we found a significant enrichment of chimpanzee genes with differential expression in lymphoblastoid cell lines and induced pluripotent stem cells, both within deletions and near inversion breakpoints. We examined chromatin-conformation maps from human and chimpanzee using these same cell types and observed alterations in genomic interactions at SV breakpoints. Finally, we focused on 56 genes impacted by SVs in >90% of chimpanzees and absent in humans and gorillas, which may contribute to chimpanzee-specific features. Sequencing a greater set of individuals from diverse subspecies will be critical to establish the complete landscape of genetic variation in chimpanzees.The Pelibuey sheep has adaptability to climatic variations, resistance to parasites, and good maternal ability, whereas some ewes present multiple births, which increases the litter size in farm sheep. The litter size in some wool sheep breeds is associated with the presence of mutations, mainly in the family of the transforming growth factor β (TGF-β) genes. To explore genetic mechanisms underlying the variation in litter size, we conducted a genome-wide association study in two groups of Pelibuey sheep (multiparous sheep with two lambs per birth vs. uniparous sheep with a single lamb at birth) using the OvineSNP50 BeadChip. We identified a total of 57 putative SNPs markers (p less then 3.0 × 10-3, Bonferroni correction). The candidate genes that may be associated with litter size in Pelibuey sheep are CLSTN2, MTMR2, DLG1, CGA, ABCG5, TRPM6, and HTR1E. Genomic regions were also identified that contain three quantitative trait loci (QTLs) for aseasonal reproduction (ASREP), milk yield (MY), and body weight (BW). These results allowed us to identify SNPs associated with genes that could be involved in the reproductive process related to prolificacy.Graphene oxide (GO) is a chemical compound with a form similar to graphene that consists of one-atom-thick two-dimensional layers of sp2-bonded carbon. Graphene oxide exhibits high hydrophilicity and dispersibility. Thus, it is difficult to be separated from aqueous solutions. Therefore, functionalization with magnetic nanoparticles is performed in order to prepare a magnetic GO nanocomposite that combines the sufficient adsorption capacity of graphene oxide and the convenience of magnetic separation. Moreover, the magnetic material can be further functionalized with different groups to prevent aggregation and extends its potential application. Until today, a plethora of magnetic GO hybrid materials have been synthesized and successfully employed for the magnetic solid-phase extraction of organic compounds from environmental, agricultural, biological, and food samples. The developed GO nanocomposites exhibit satisfactory stability in aqueous solutions, as well as sufficient surface area. Thus, they are considered as an alternative to conventional sorbents by enriching the analytical toolbox for the analysis of trace organic compounds.
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