Notes

Structures Peaks Spectra Fractions
Each of the four oligosaccharides OS-5, OS-6, OS-7-1, and OS-7-2 contained an alpha-D-galactofuranosyl residue (Galf) linked to Man(A) via an alpha-(1--2)-linkage. OS-7 was found to consist of two oligosaccharides. The structures of these four oligosaccharides were determined to be GalfMan5GlcNAc2, GalfMan6GlcNAc2, GalfMan7GlcNAc2, and GalfMan8GlcNAc2 by 1H NMR spectroscopy and compositional analysis. The Galf structure of GalfMan5GlcNAc2 was found to be identical to that of an oligosaccharide previously isolated from the alpha-D-galactosidase of the same strain. The structure of OS-3 remains Enzymic removal of two oligosaccharide chains from ricin B-chain.PeptideN-glycosidase F removed both the asparagine-linked oligosaccharide chains of ricin B-chain in the absence of lactose.

In the presence of lactose, which binds specifically to the B-chain, only one oligosaccharide chain was removed. Lactose also protected Ricinus communis agglutinin B-chain against the removal of one of the two susceptible oligosaccharides present in each B-chain Characterization of seven xyloglucan oligosaccharides containing from seventeen The complete primary structures of seven oligosaccharide subunits of the xyloglucan secreted by suspension-cultured Acer pseudoplatanus cells were determined. The oligosaccharides, ranging in size from 17 to glycosyl residues, were generated by treatment of the xyloglucan with an endo-beta-(1----4)-glucanase. The oligosaccharide components of a fraction obtained by Bio-Gel P-2 chromatography of enzyme-treated xyloglucan were further purified by normal-phase h.p.l.c.

and then converted to the corresponding oligoglycosyl alditols by reduction with NaBH4. The oligoglycosyl alditols, after purification to near homogeneity by reversed-phase h.p.l.c., were structurally characterized by 1H-n.m.

r. spectroscopy, fast-atom bombardment mass spectrometry (f.a.b.-m.s.), and analysis of their glycosyl-residue and glycosyl-linkage compositions.

Novel structural elements of xyloglucans were observed in this study, including beta-D-xylopyranosyl and alpha-L-arabinofuranosyl-(1----3)-beta-D-xylopyranosyl sidechains. The results also extend our list of correlations between 1H-n.m.r. resonances and specific structural features of xyloglucans and thus enhance our ability to determine the structures of xyloglucans from various sources.Catalog-library approach for the rapid and sensitive structural elucidation of We obtained the nearly complete structural elucidation of oligosaccharide components, including sequence, linkage, and even stereochemistry in the picomolar levels. The catalog-library approach is used for elucidating the structures of minor components in a mixture of oligosaccharides.

Oligosaccharides released from a family of glycoproteins are often composed of a small finite set of monosaccharides. In this regard, the numerous oligosaccharide species are analogous to the products found in syntheses involving combinatorial libraries. The great structural diversity in the library is the result of the nearly infinite combinations in which even a small number of monosaccharides can be arranged. Fortunately, structural similarities exist between different oligosaccharides, as specific substructural motifs are preserved among different compounds. We propose that a catalog of substructural motifs can be identified and characterized by collision-induced dissociation mass spectrometry. The catalog is constructed from a set of known compounds that have been fully structurally elucidated by, for example, nuclear magnetic resonance. The catalog consists of the characteristic fragmentation patterns belonging to a set of specific substructural motifs.

Collision-induced dissociation is used to determine the presence of these motifs and reconstruct the structures of less abundant components.Measurement of endo-α-mannosidase activity using a fluorescently labeled Endo-α-mannosidase, a GH99-family glycoside hydrolase, cleaves α-mannoside linkages with glucose residues. This enzyme is proposed to play a critical role in N-glycan processing for deglucosylation. To measure endo-α- Lactose-N-neotetraose , we synthesized a fluorescently labeled tetrasaccharide derivative tetrasaccharide skeleton was prepared by step-wise coupling using mannose donors 4 and 7. The 1,2-cis α-glycosidic linkage on the non-reducing end of the glucose residue was constructed by inversion of the stereochemistry of the C-2 hydroxyl group in the α-mannose residue. Finally, the dansyl group was introduced at the reducing end via an aminopropyl linker. lacto-n-neotetraose measured Biosynthesis of lipid-linked oligosaccharides in cotton fibers.
Homepage: https://en.wikipedia.org/wiki/Lacto-N-tetraose