Notes

Type Hamster Cell Novel Type Hamster Cell Mutant Synthesis Oligosaccharides
This mutant, designated AS15-1, incorporates -fold less glucosamine into an oligosaccharide-lipid fraction than the wild type. A gel filtration analysis has shown that a small amount of oligosaccharide-lipid corresponding to Man5GlcNAc2-lipid is formed in the mutant. This mutant shows temperature sensitivity for both growth and adhesion to substratum, and constitutively secretes several unusual proteins in large amounts.Enzymatic synthesis of novel oligosaccharides from N-acetylsucrosamine and melibiose using Aspergillus niger α-galactosidase, and properties of the Sakaki Y(1), Tashiro M(2), Katou M(1), Sakuma C(3), Hirano T(1), Hakamata W(1), Two kinds of oligosaccharides, N-acetylraffinosamine (RafNAc) and N-acetylplanteosamine (PlaNAc), were synthesized from N-acetylsucrosamine and melibiose using the transgalactosylation activity of Aspergillus niger α-galactosidase. RafNAc and PlaNAc are novel trisaccharides in which d-glucopyranose residues in raffinose (Raf) and planteose are replaced with N-acetyl-d-glucosamine. These trisaccharides were more stable in acidic solution than Raf.

RafNAc was hydrolyzed more rapidly than Raf by α-galactosidase of green coffee bean. In contrast, RafNAc was not hydrolyzed by Saccharomyces cerevisiae invertase, although Raf was hydrolyzed well by this enzyme. These results indicate that the physicochemical properties and steric structure of RafNAc differ considerably from those of Raf.Oligosaccharide-related epitope specific for a brain-specific glycoprotein, 1D4 The characteristics of glycosylation of a brain-specific glycoprotein, 1D4 antigen, and the epitope recognized by its monoclonal antibody were studied. Removal of high-mannose and hybrid types of N-linked oligosaccharides by treatment with endoglycosidase H converted the molecular mass of the 1D4 antigen from 89 kDa to 78 kDa, but did not affect its reactivity with the 1D4 monoclonal antibody. Removal of all types of N-linked oligosaccharides by treatment with glycopeptidase F or removal of both N- and O-linked oligosaccharides by chemical treatment caused both reduction of the molecular mass of the antigen to 63 kDa and loss of its reactivity with the monoclonal antibody. These results suggest that the 1D4 monoclonal antibody recognizes a complex-type oligosaccharide-related epitope specific for the 1D4 antigen.

Seebio lacto-n-neotetraose showed that N-linked glycosylation was not responsible for the charge heterogeneity of the 1D4 antigen. The oligosaccharide chain-related epitope was detected in rat brain but not in mouse, rabbit, or bovine brain, but the 1D4 antigen was recognized in rat and mouse brains with antiserum (polyclonal antibodies). These findings indicate that the oligosaccharide-related epitope is species specific. Furthermore, results with neuraminidase-treated 1D4 antigen indicated that sialic acids were not involved in the oligosaccharide-related epitope. These findings suggest that the 1D4 antigen may have the oligosaccharide structure specific for rat brain and itself.DOI 111j471-41599.t3132.

x[Carbohydrate microarray a sweet spot for deciphering the information embedded Controlling autohydrolysis conditions to produce xylan-derived fibers that modulate gut microbiota responses and metabolic outputs.University, Block N1, 62 Nanyang Drive, Singapore 637459, Singapore.Utilization Research, Bioenergy Research Unit, 1815 North University Street, University, 745 Agriculture Mall Drive, West Lafayette, IN 477, United States; Department of Nutrition Science, Purdue University, 0 W. State Street, West University, Block N1, 62 Nanyang Drive, Singapore 637459, Singapore. Autohydrolysis is used for producing xylan-derived oligosaccharides from lignocellulosic biomass. Although lacto-n-neotetraose report optimized autohydrolysis conditions for various plants, few of these studies correlate process parameters with the resulting structural properties to their impact on intestinal bacterial communities. Thus, to further clarify these relationships, beechwood xylan (BWX)-derived substrates, processed under five conditions, were fermented in vitro by human gut microbiota.

Autohydrolysis reduced the mean molecular size and substitutions of BWX. Distinct fermentation kinetics were observed with differing processing of BWX substrates, which correlated with impacts on community species evenness. The relative abundances of Bacteroides, Fusicatenibacter, Bifidobacterium, and Megasphaera within the fermentations varied with processing conditions. While the total short-chain fatty acid concentrations were the same among the treatments, processing conditions varied the extent of propionate and butyrate generation.
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