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Metabolomics as well as physiological looks at validates previous studies on the system associated with reaction to wounding anxiety of different intensities throughout broccoli.
A far-red fluorescence "off-on" sensing strategy is described for sequential ratiometric determination of Cu2+ and L-histidine (L-His) based on fluorescence resonance energy transfer (FRET) system. N,S,P co-doped carbon dots (N,S,P-CDs) and N-acetyl-L-cysteine functionalized gold nanoclusters (NAC-AuNCs) are used in the FRET system, which serve as energy donor and acceptor, respectively. After adding NAC-AuNCs into the solution of N,S,P-CDs, the fluorescence of N,S,P-CDs is effectively quenched, while the far-red fluorescence of NAC-AuNCs appears. Cu2+ can decrease fluorescence of NAC-AuNCs, and then L-His can effectively recover the fluorescence of NAC-AuNCs. The possible reason is that the stronger affinity between Cu2+ and L-His can pull Cu2+ away from the surface of NAC-AuNCs. Through it all, the emission intensity of N,S,P-CDs remains nearly constant, so the ratio of fluorescence intensities at 485 and 625 nm exhibits a linear correlation to the Cu2+ and L-His concentration, respectively. The sensing platform shows good selectivity towards Cu2+ and L-His with a linear range of 0.65-26.58 μM and 3.13-56.25 μM and determination limits of 0.50 μM and 0.374 μM, respectively. The proposed method has been successfully used for Cu2+ and L-His determination in real samples with satisfying results. Graphical abstract.PURPOSE Orthorexia nervosa (ON) is described as an obsession for healthy eating with potentially debilitating consequences but little of its psychopathology is empirically supported. Using suitable validation methodologies, we wanted to optimize the empirical assessment of ON symptoms and investigate their unclear relationship with BMI. Our objective was, therefore, twofold (1) Validation of a robust measurement model of ON dimensions using the Eating Habits Questionnaire (EHQ); (2) Validation of a structural model describing a mechanism of relationships between ON dimensions and BMI. METHODS A self-report questionnaire assessing BMI and ON through a French translation of the 21-item EHQ was administered to a large sample of French adults (N = 2065). We used Exploratory and Confirmatory Factor Analysis for objective no. 1 and Structural Equation Modeling for objective no. 2. RESULTS We validated a well-fitted (TLI = 0.98; RMSEA = 0.04) and conceptually consistent measurement model with 16 items for three ON dimensions Rigid Eating Behavior (REB), Positive Feeling of Control (PFC) and Problems of Attention Control and Social Relationships (PACSR). We also validated a structural model (TLI = 0.96; RMSEA = 0.05) showing that 1. REB strongly impacts both PFC and PACSR; 2. REB has a significant negative impact on BMI and BMI has a significant positive effect on PACSR but effect sizes are very small and globally ON dimensions are only marginally related to BMI. CONCLUSION Our study achieved an improved assessment method of ON, a clarification of its links with BMI and implications for the descriptive psychopathology of ON. LEVEL OF EVIDENCE V, Descriptive cross-sectional study.BACKGROUND Many drugs with dose-dependent effects on hemodynamic variables are metabolized by cytochrome P450 2D6 (CYP2D6). The aim of this study was to compare prescribed dosages and hemodynamic responses of such drugs in relation to pharmacogenetic variability in CYP2D6 metabolism among patients aged ≥ 70 years exposed to polypharmacy. MATERIALS AND METHODS We included 173 patients with detailed information about drug use. The patients were retrospectively subjected to CYP2D6 genotyping, which comprised the most common variant alleles encoding reduced, absent, or increased CYP2D6 metabolism. In order to compare dosages across different CYP2D6-metabolized drugs, all prescribed daily doses were harmonized to the 'percent of a daily defined dose' (DDD). The mean harmonized DDD was compared between genotype-predicted normal metabolizers (NMs) and patients with reduced or absent CYP2D6 enzyme activity, defined as intermediate or poor metabolizers (IMs/PMs). Elenbecestat nmr Blood pressure, pulse, and patient proportions with orthostatism and bradycardia were also compared between genotype subgroups. RESULTS The genotype-predicted phenotype subgroups comprised 79 NMs (45.7%), 75 IMs (43.4%), and 16 PMs (9.2%). There were no differences in dosing of CYP2D6 substrates between NMs and IMs/PMs (p = 0.76). A higher proportion of CYP2D6 IMs/PMs experienced orthostatism (p = 0.03), while there were no significant subgroup differences for the other hemodynamic variables. CONCLUSION In this real-life clinical setting of patients aged ≥ 70 years, dosing of CYP2D6 substrates were not adjusted according to genotype-predicted CYP2D6 metabolism. The increased occurrence of orthostatism in patients with reduced/absent CYP2D6 metabolism may indicate that individualized dosing based on genotype has the potential to prevent adverse effects in these vulnerable patients.OBJECTIVES To compare the diagnostic value of the SARC-F, MRSA-7 and MRSA-5 questionnaires in screening for sarcopenia in inpatients with chronic heart failure (CHF). PATIENTS A total of 355 CHF patients hospitalized from January 2019 to August 2019 who met the study's selection criteria were included in the analysis. MEASUREMENTS Handgrip strength and gait speed were measured, and bioelectrical impedance analysis (BIA) was used to estimate appendicular skeletal muscle mass. The sensitivity/specificity of the SARC-F, MRSA-7 and MRSA-5 questionnaires was evaluated. RESULTS The diagnostic criteria of the Asia Working Group for Sarcopenia (AWGS) were used as the gold standard for diagnosing sarcopenia. The prevalence of sarcopenia was 55.8% according to the AWGS diagnostic criteria, 31.0% according to the SARC-F, 73.0% according to the MRSA-7, and 71.3% according to the MRSA-5. Using the AWGS criteria as the gold standard, the SARC-F had a sensitivity of 52.5% and a specificity of 96.2% in the whole study population, the MRSA-7 had a sensitivity of 92.4% and a specificity of 51.6%, and the MRSA-5 had a sensitivity of 93.9% and a specificity of 57.3%. The areas under the ROC curves for the SARC-F, MRSA-7 and MRSA-5 were 0.78, 0.74 and 0.77, respectively. CONCLUSIONS The MSRA-7 and MSRA-5 may serve as novel screening tools for sarcopenia in hospitalized patients with CHF. The SARC-F, a classic screening tool, is also suitable for this population. The MSRA-7 and MSRA-5 have better sensitivity, whereas the SARC-F has better specificity.
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