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Sexed semen played only a minor role for production traits in first lactations. The extrapolated 305-day milk yield was 200 kg lower for first calf heifers, which were inseminated with sexed semen compared to heifers inseminated with conventional semen. Fat and protein yield were 6 kg to 8 kg lower after use of sexed semen. Animals with female offspring from sexed semen showed higher survival rates than the other groups.For some wild canids, such as the Iberian wolf, there is a lack of in-depth knowledge about dental pathology. We aimed to evaluate it, in a standardized manner, in specimens from a Portuguese museum collection. Sixty-five deceased specimens of wild Iberian wolves, 61 complete skulls and 4 mandibles, collected in Portugal between 1977 and 1995, were analyzed. Sample comprised 18 females, 24 males and 23 individuals of undetermined sex. Teeth were evaluated by visual observation and dental radiography for tooth wear, periodontitis, fractures and other dental lesions. We have found several causes for teeth absence artefactual, secondary to periodontitis and agenesia. About 30% of the teeth showed signs of wear. Only a small ( less then 13%) fraction of maxillary and mandibular teeth did not show periodontitis. The tooth 308 showed periodontitis in all males (p = 0.017) and the tooth 104 was significantly affected by this condition in females (p = 0.020). A significant relationship was found between females and tooth wear in three teeth. Periodontitis showed a significant association with tooth wear (p less then 0.001) and fractures (p = 0.027). Tooth fractures were more frequent in the maxilla than in the mandible. Protein Tyrosine Kinase inhibitor Seven periapical lesions, seven root fusions and three specimens with malocclusion were identified in the collection. Results are discussed integrating information from diet, habitat, genetic and spatial behavior. Dental radiography is here proposed as an approach for the age estimation in archaeological canids. This research contributes to the knowledge of the dental disease in the largest wolf population in Western Europe, a target subspecies of multiple conservation measures.Collection of pooled oral fluid (OF) by allowing pigs to chew on a cotton rope is an alternative to blood sampling. However, little is known about the applicability of this method to suckling piglets. The objectives of the present study were to describe the spontaneous interaction of suckling piglets with a rope and to investigate the influence of a rope pre-exposure on the success rate of sampling. We studied the interaction dynamics of 21 and 28 days-old suckling piglets with a cotton rope presented for 30 min. Ropes were manually wrung out inside plastic bags to release the oral fluid. A total of 49 litters were included. Percentages of success of pooled OF collection for 28-day-old, 21-day-old and 21-day-old pre-exposed litters were 82%, 62% and 100%, respectively. The mean volume collected did not differ between groups. Without pre-exposure, 84.7% and 95% of piglets interacted spontaneously with the rope at 21 and 28 days of age, respectively. The latency between rope presentation and interaction was highly variable between piglets within litters from less then 10 s to 30 min. Among piglets having interacted with the rope, the interaction lasted for at least 60 s for 90% and 91.4% of 21 and 28-day-old piglets, respectively. Pooled OF collection is achievable prior to weaning in piglets of at least 21 days of age. Pooled OF sampling is representative at litter level if collection is successful. In order to improve the success rate of collection, pre-exposing the piglets with a rope one day prior to sampling is effective.Dairy cattle production is distributed throughout the Brazilian regions. However, Brazilian producing regions are different in air temperature and humidity, diet, birth season, and other factors that may alter the reproductive performances of cows. The age of the cow at first calving (AFC) is a good indicator of sexual precocity since it shows the beginning of the female reproductive life and has a great influence on cow replacement costs. Researches on genotype-environment interaction (GEI) show the importance of using specific bulls for the different production systems in Brazil since most semen used in the country is imported. The objective of this work was to evaluate GEI for AFC in Holstein cows in Brazil, using reaction norms. The statistical models used were the standard animal model, which disregards the GEI, and hierarchical reaction norm models with homoscedastic (HRNMHO) and heteroscedastic (HRNMHE) residual variance, and one (HRNMHO1S and HRNMHE1S) and two (HRNMHO2S and HRNMHE2S) steps. HRNMHO1S presented better fit to the data, with lower heritability for environments with lower AFC, and higher heritability for environments with higher AFC. The GEI found was complex, with a reclassification of bulls, denoting the importance of considering GEI for evaluation and selection of bulls for different production levels. The reduction of AFC is possible when using breeding bulls adapted to the tropical and subtropical conditions of Brazil.This study investigated the effect of supplementation of ewes with calcium (Ca) and magnesium (Mg) in late gestation and early lactation on the plasma mineral concentration, hormone profile and immunity responses of ewes and their lambs. Twin bearing ewes were allocated between four dietary treatment groups (n = 11 per treatment) control (0.33% DM Ca and 0.28% DM Mg); high Ca (0.72% DM Ca and 0.28% DM Mg); high Mg (0.33% DM Ca, 0.48% DM Mg); and high Ca+Mg (0.66% DM Ca and 0.47% DM Mg), offered as part of a whole ration. Ewes were fed the treatment supplement from around one month prior to lambing to one month after lactation. Blood and urine samples were collected at seven time-points weekly starting at 5 weeks prior to lambing; within 12 h post lambing (+12 h); and then at fortnightly intervals at 2 week (+2 W) and at 4 week (+4 W) post lambing. Colostrum/milk samples from ewes and blood samples from lambs were collected at +12 h, +2 W and +4 W. Live weight of lambs were measured at +12 h, +2 W and +4 W. The plasma concentration of PTH, 1,25(OH)2D3 and 25(OH)D3 was lowest at +2 W (P ≤ 0.
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