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The formaldehyde dehydrogenase (FADH) activity in plants is essential to the removal of airborne formaldehyde (FA) by plants. A rapid and efficient method was established to assess the FADH activity in plants by analyzing the efficiencies of the extracts of fresh and enzyme-inactivated leaves to degrade FA, with the enzyme-inactivated leaves prepared by freezing with liquid nitrogen. The efficiencies of airborne FA dissipated by different plants were evaluated through the FA fumigation experiments using four selected plants, with the results analyzed against the calculated leaf FADH activities. Fresh and enzyme-inactivated leaf extracts degraded FA to different extents. The degradative efficiencies of leaf extracts were positively related to the initial FA test levels at 6-18 mg l-1. The relative plant-leaf FADH activities formed the order of Chenopodium album L. > Atenia cordifolia > Plantain > Aloe, which was in line with the observed FA dissipating efficiencies of the plants exposed to 0.72 mg m-3 airborne FA for 24 h. Other dominant degrading mechanisms in plant leaves resulted in higher dissipating efficiencies of Plantain over that of Atenia cordifolia when exposed to 1.56 mg m-3 FA for 24 h. The established method could be applied to estimate the FADH activity in plants for assessment of the plant remediation efficiency of FA in air at lower concentrations.The aim of current research was to explore different fungi as plant growth promoting fungi (PGPF). Strains of Trichoderma are well explored till now. But there are few other fungal strains that are better than Trichoderma strains. The study involves the isolation of different fungi from the rhizosphere of various agriculture farms. After isolation, 18S rRNA identification was carried out. Isolated fungi belonged to genus Penicillium, Talaromyces, Trichoderma, and Aspergillus. Isolate M13 belonging to genus Talaromyces was screened for its plant growth promoting (PGP) activity as it is a novel strain and still to be explored. Isolate M13 was identified as Talaromyces pinophilus (MG011365). Indole acetic acid (IAA) estimation was carried out using Salkowski reagent. Isolate was allowed to grow in cultivation media (Potato Dextrose Broth, PDB) in which one was supplemented with tryptophan (TRP) and one was without TRP. Phosphate solubilization was assessed using Pikovskaya's media and latter estimated using stannous chloride method, showing decent solubilization of phosphate. Siderophore production was assessed using CAS assay that indicated decent extent of siderophore production. Further for biocontrol, enzymatic assay for β-glucanase and chitinase was carried out. For β-glucanase enzyme production 1% carboxymethylcellulose (CMC) and for chitinase enzyme production 10% v/v colloidal chitin (as a sole carbon source) supplemented in solid minimal-medium-9 (MM9) were used. Antagonism effect of isolate M13 was carried out against Aspergillus niger by dual cultural method. As the strain M13 showed several PGP traits, liquid bioformulation was prepared to perform seed germination assay and pot trials on chickpea, followed by field trial on banana plant. All the experimental data of biochemical assessment with pot and field trials suggest T. pinophilus M13 as a novel fungus that can be used as biointensifier.Significant levels of polycyclic aromatic hydrocarbons (PAHs) were detected in lakes. The competition between algae would be disturbed by PAHs resulted in variations of algal growth. For controlling the cyanobacterial blooms, it is important to understand this disturbed competition between Microcystis aeruginosa and other algae. A 6-day cultivation experiment was designed to investigate the responses of M. aeruginosa to PAHs in presence of green algae. STAT inhibitor A popular green alga Chlorella pyrenoidosa was used as a representative of green algae, and phenanthrene and pyrene were selected as representatives of PAHs. The results showed that M. aeruginosa outcompeted C. pyrenoidosa under PAH contamination, and PAHs and M. aeruginosa significantly inhibited the survival of C. pyrenoidosa. PAHs disturbed the growth of algae by influencing photosynthetic pigments and phycobiliproteins, and the different alteration of Fv/Fm ratios implied that shifted algal community composition would be induced by PAHs. The Fv/Fm of the two algal mixture and individual C. pyrenoidosa was significantly negatively correlated with phenanthrene levels. However, there were no significant correlations between the Fv/Fm of M. aeruginosa and the exposure levels of phenanthrene or pyrene. Remarkably, the Fv/Fm significantly increased in M. aeruginosa at 0.15 mg L-1 pyrene, suggesting that PSII resistance to pyrene was enhanced in M. aeruginosa. Our results pointed out an increasing frequency and intensity of cyanobacterial blooms could be induced by PAHs in contaminated waters.Phthalate esters (PAEs) are widely used in the manufacturing of plastics, and their demands have grown rapidly, especially in China, which will lead to much more environmental pollution of PAEs. In this study, fourteen common PAEs in ambient air were investigated during non-typhoon and typhoon seasons in a mixed multi-functional area of Hangzhou, China. The average concentrations of ∑14 PAEs in gaseous and PM2.5-bound phase (G-PAEs and P-PAEs) were 2317 ng/m3 and 128 ng/m3 during sampling period, while the mean concentrations of total PAEs in non-typhoon and typhoon seasons were 2412 ng/m3 and 2183 ng/m3, respectively. Bis(2-ethylhexyl)phthalate (DEHP) was the most abundant one, averagely accounting for 63.2% of G-PAEs and 88.3% of P-PAEs. Relative humidity showed a significant negative correlation with short-chain PAE (r = - 0.479, P less then 0.01) and long-chain PAE (r = - 0.305, P less then 0.05) concentrations in non-typhoon and typhoon seasons, and O3 could degrade G-PAEs through photoreaction. Source identification by the positive matrix factorization model and conditional probability function indicated that P-PAEs were mainly from the release from indoor environment (43%), PVC source (34%), construction source (12%), and industry source (11%). Air mass transport from both inland and oceans affected the PAE pollution in non-typhoon season, while its long-range transport from oceans took an important role in typhoon season. The daily inhalation intakes of PAEs for infants, teenagers, and adults were estimated, which showed that infants experienced the highest exposure risk.
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