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ol induction in C. lini and would guide strain engineering to further improve dihydroxylation efficiency.Scanning ion conductance microscopy (SICM), as an emerging non-contact in situ topography measurement tool with nano resolution, has been increasingly used in recent years in biomedicine, electrochemistry and materials science. In the conventional measurement method of SICM, the sample topography is constructed according to the position of the probe at the feedback threshold of the ion current. Nevertheless, for different structures, a constant threshold cannot maintain a constant probe-sample distance. This phenomenon makes the measurement topography inconsistent with the real sample surface. In order to solve this problem and improve the measurement accuracy of SICM, a new ion conductance imaging method based on the approach curve spectrum is proposed in this work. In the new method, the local feature around the measurement point is firstly evaluated according to the change rate of ion current. Secondly, based on the local feature, the corresponding approach curve is searched from the prior approach curve spectrum to accurately evaluate the distance between the probe and the sample. find more Finally, the sample topography is constructed by the probe position subtracting the probe-sample distance. In this paper, we verify the feasibility of the new imaging method by combining finite element theory and experiments. To examine the measurement accuracy, the standard strip silicon and cylindrical polydimethylsiloxane (PDMS) samples are tested, and the improved imaging method can obtain the topography closer to the real samples and reduce the volumetric measurement error by 5.4%. The implementation of the new imaging method will further promote SICM application in related research fields.Abbe theory of image formation can be expanded from two-dimensional to three-dimensional space. It has to consider an optical system that forms an image of a grating in an object space. It is possible to introduce various inclinations of this grating relatively the optical axis round the axis in the object plane that is parallel to its slits. In this case, the minimum resolvable period and the corresponded spatial cut off frequency of the optical system may be calculated as the functions of the angle of grating inclination, the numerical apertures and the wavelength using the idea of Abbe theory. In case of axial symmetry of the optical system, the function for calculation of the spatial cut off frequencies describes a surface in the three-dimensional space of spatial frequencies that covers the three-dimensional spatial bandwidth. The spatial harmonics with spatial frequencies inside this spatial bandwidth can pass through an optical system and they participate in formation of three-dimensional images. The other spatial harmonics cannot pass through it. This approach makes understanding and explanation of formation of three-dimensional images clearer, faster and logically completed.STEM imaging is typically performed by raster scanning a focused electron probe over a sample. Here we investigate and compare three different scan patterns, making use of a programmable scan engine that allows to arbitrarily set the sequence of probe positions that are consecutively visited on the sample. We compare the typical raster scan with a so-called 'snake' pattern where the scan direction is reversed after each row and a novel Hilbert scan pattern that changes scan direction rapidly and provides an homogeneous treatment of both scan directions. We experimentally evaluate the imaging performance on a single crystal test sample by varying dwell time and evaluating behaviour with respect to sample drift. We demonstrate the ability of the Hilbert scan pattern to more faithfully represent the high frequency content of the image in the presence of sample drift. It is also shown that Hilbert scanning provides reduced bias when measuring lattice parameters from the obtained scanned images while maintaining similar precision in both scan directions which is especially important when e.g. performing strain analysis. Compared to raster scanning with flyback correction, both snake and Hilbert scanning benefit from dose reduction as only small probe movement steps occur.The mechanisms and enhancing effects of different biochar loadings on the digesters receiving low and high excess (or anaerobic) sludge loadings were thoroughly examined in the present study. This was done to explore an efficient method for converting excess sludge to anaerobic sludge. Biochar had an obvious effect on the anaerobic digestion of excess sludge but not on the anaerobic sludge. When the amount of biochar added was equivalent to 100% of the sludge TS, the cumulative methane yields of anaerobic digestion inoculated with small and large amounts of excess sludge were respectively 30.2 and 1.7 times that of those without biochar. The number of methanogens in the digesters that received small and large inoculations of excess sludge with 100% biochar, were respectively 105.4% and 20.6% higher than those without biochar. The biochar enhanced the systems performance because it selectively enriched the Trichococcus and Methanomicrobiales tightly attach to it. This enhanced the synergy and overall activity of the system by promoting biofilm development. Ultimately, the integration of 100% biochar and excess sludge can be used as a substitute for anaerobic sludge as an inoculum by giving similar overall performance.Erythromycin fermentation residue (EFR) is the precipitation of fermentative biowaste used for extracting erythromycin (ERY) and may be disposed via land application after heat-activated persulfate (PS) oxidation treatment. However, the effects of the treated EFR as a soil amendment on soil chemical properties and the potential resistance risks caused by introduced ERY remain unclear. Here, a laboratory soil incubation experiment was performed to investigate the soil pH, salinity, introduced antibiotics, antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs), as well as bacterial community structure in the treated EFR-amended soil. The results indicated that pH in treated EFR-amended soil decreased firstly and then increased. The salinity of soil increased but soil was still non-saline soil. In addition, the introduced ERY in the treated EFR-amended soil decreased with the half-life of 12.3 d. Moreover, the relative abundances of ERY resistance genes and MGEs in the treated EFR-amended soil were much lower than those in the control at the end of incubation.
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