Notes

Accidental swallowing of swamp natural stone: A whole new and unsafe source of direct poisoning.
Human preimplantation development is a complex process involving dramatic changes in transcriptional architecture. For a better understanding of their time-spatial development, it is indispensable to identify key genes. Although the single-cell RNA sequencing (RNA-seq) techniques could provide detailed clustering signatures, the identification of decisive factors remains difficult. Additionally, it requires high experimental cost and a long experimental period. Thus, it is highly desired to develop computational methods for identifying effective genes of development signature. In this study, we first developed a predictor called EmPredictor to identify developmental stages of human preimplantation embryogenesis. First, we compared the F-score of feature selection algorithms with differential gene expression (DGE) analysis to find specific signatures of the development stage. In addition, by training the support vector machine (SVM), four types of signature subsets were comprehensively discussed. The prediction results showed that a feature subset with 1,881 genes from the F-score algorithm obtained the best predictive performance, which achieved the highest accuracy of 93.3% on the cross-validation set. Further function enrichment demonstrated that the gene set selected by the feature selection method was involved in more development-related pathways and cell fate determination biomarkers. This indicates that the F-score algorithm should be preferentially proposed for detecting key genes of multi-period data in mammalian early development. The senescence-accelerated mouse prone 8 (SAMP8) mouse model is a useful model for investigating the fundamental mechanisms involved in the age-related learning and memory deficits of Alzheimer's disease (AD), while the SAM/resistant 1 (SAMR1) mouse model shows normal features. Recent evidence has shown that long non-coding RNAs (lncRNAs) may play an important role in AD pathogenesis. However, a comprehensive and systematic understanding of the function of AD-related lncRNAs and their associated nearby coding genes in AD is still lacking. In this study, we collected the hippocampus, the main area of AD pathological processes, of SAMP8 and SAMR1 animals and performed microarray analysis to identify aberrantly expressed lncRNAs and their associated nearby coding genes, which may contribute to AD pathogenesis. We identified 3,112 differentially expressed lncRNAs and 3,191 differentially expressed mRNAs in SAMP8 mice compared to SAMR1 mice. More than 70% of the deregulated lncRNAs were intergenic and exon sense-overlapping lncRNAs. Gene Ontology (GO) and pathway analyses of the AD-related transcripts were also performed and are described in detail, which imply that metabolic process reprograming was likely related to AD. Furthermore, six lncRNAs and six mRNAs were selected for further validation of the microarray results using quantitative PCR, and the results were consistent with the findings from the microarray. Moreover, we analyzed 780 lincRNAs (also called long "intergenic" non-coding RNAs) and their associated nearby coding genes. Among these lincRNAs, AK158400 had the most genes nearby (n = 13), all of which belonged to the histone cluster 1 family, suggesting regulation of the nucleosome structure of the chromosomal fiber by affecting nearby genes during AD progression. In addition, we also identified 97 aberrant antisense lncRNAs and their associated coding genes. It is likely that these dysregulated lncRNAs and their associated nearby coding genes play a role in the development and/or progression of AD. The pregnancy-specific disorder preeclampsia is believed to affect 2-8% of pregnancies worldwide. The only known cure is delivery of the fetus and placenta. This brief communication cites evidence that might suggest a moderately effective treatment for preeclampsia. The therapy consists of having the gravida with preeclampsia stay in a room provided with FACE (Free Air Carbon-dioxide Enrichment). check details No containment (such as in greenhouses) is required with FACE systems. Evidence is cited which suggests that the therapy might reduce the incidence of preeclampsia by perhaps 10-30% without much harm to the fetus. The influences of substituent and solvent polarity on the electronic transition of seven different 4-aminoantipyrine based Schiff bases have been investigated in 14 solvents of different polarity. Reichardt'sET(30) scale has been used to propose a quantitative approach towards the relative stability of the electronic ground and excited state species. The Schiff bases can sense the polarity changes of the solvents and accordingly there appear three categories of solvents non-polar, dipolar aprotic and polar protic. The first two categories of solvents induce negative solvatochromism and the third one induces positive solvatochromism in the molecules. Cross conjugation and geometrical configuration have been proposed to play an important role in the extent of electronic transition of the Schiff bases in various solvent media. Out of various substituent effects, field effect and resonance significantly influence the electronic environment of the compounds, as evidenced from the regression models involving change in UV-Vis absorption wavelengths 1HNMR chemical shifts and IR frequencies. Two new smart spectrophotometric methods are developed and validated for the determination of the quatertnary mixture of paracetamol (acetaminophen), diphenhydramine, p-aminophenol, and N-oxide degradate of diphenhydramine. Method A is the novel triple divisor ratio difference method, where the triple divisor ratio spectrum of the component of interest shows a significant amplitude difference at two selected wavelengths where the three interfering substances are used as triple divisor and give constant amplitude all over the spectrum. The triple divisors are normalized spectra of tertiary mixtures containing 40 μg/mL of each of the 3 interfering components. The selected wavelengths are 256-290 nm, 220-230 nm, 230-245 nm and 275-260 nm for the 4 components, respectively. Method B is double divisor - ratio difference-dual wavelength, where the double divisor ratio spectrum of the component of interest shows a significant amplitude difference at two selected wavelengths where two interfering substances are used as double divisor and give constant amplitude all over the spectrum, while the third one shows zero amplitude difference at these two selected wavelengths.
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