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Speedy One-Pot Diagnosis involving SARS-CoV-2 With different Side to side Flow Assay in Clinical Samples.
Cross-cultural adaptation of the National Early Warning Score 2 to Brazilian Portuguese.

A methodological study of a cross-cultural adaptation of a scale, based on the Beaton et al. framework, authorized by the Royal College of Physicians. Judges from nine Brazilian states, nurses and physicians evaluated the semantic, idiomatic, cultural, and conceptual equivalence between the original instrument and the translated versions. The nurses, working in inpatient or emergency units, conducted the pilot test, applying the final version to three case studies. Psychometric tests were used for data analysis Content Validity Index (CVI), Kappa Coefficient, and Cronbach's Alpha.

The adaptation showed a mean CVI of 0.98 and perfect/almost perfect inter-rater agreement, with scores above 0.80. The consistency of the scale was 0.712.

The process of cross-cultural adaptation of the scale to Brazilian Portuguese was successful, providing Brazilian professionals with an instrument aligned with patient safety.
The process of cross-cultural adaptation of the scale to Brazilian Portuguese was successful, providing Brazilian professionals with an instrument aligned with patient safety.
To verify the accuracy of the Manchester Triage System (MTS) and the outcomes of adult patients in an emergency hospital service.

Cross-sectional study, conducted through an inspection of records of risk classification of adult patients treated in the emergency service of a hospital.

The patients (n = 400) were classified according to priority levels, in red (0.8%), orange (58.2%), and yellow (41.0%). The accuracy levels between auditors and nurses were substantial for the flowchart (K = 0.75), and moderate for discriminating factors (k = 0.46) and priority levels (k = 0.42). Propionyl-L-carnitine mouse The accuracy of the MTS was 68.8% with regard to priority levels. Regarding outcomes, 60% of patients were discharged, 37% were transferred to other units, and 3% died.

The MTS proved to be a good predictor of the assessed outcomes, showing that 65.9% of Low Urgency patients progress to discharges, and 3.8% of High Urgency patients progress to death. The accuracy of the MTS was moderate, which suggests the need to implement inspections in emergency services.
The MTS proved to be a good predictor of the assessed outcomes, showing that 65.9% of Low Urgency patients progress to discharges, and 3.8% of High Urgency patients progress to death. The accuracy of the MTS was moderate, which suggests the need to implement inspections in emergency services.
To analyze the scientific evidence available in literature on the use of serious games for health students to learn about cardiopulmonary resuscitation.

Integrative review in PubMed / MEDLINE®, LILACS, Scopus and CINAHL databases. Included primary studies, in Portuguese, English or Spanish, from January 2009 to August 2019. The Rayyan software was used for study selection.

We identified 115 studies, and 8 composed the sample, categorizing two domains Intention of the study with regard to cardiopulmonary resuscitation through the serious game, and method used by the serious game to teach CPR.

Medical students are the main audience for serious game learning, and the main intentions of the games are to compare their effectiveness with traditional methodologies and knowledge retention. The method adopted is the description of a cardiorespiratory arrest for training. The serious game proved effective for learning cardiopulmonary resuscitation.
Medical students are the main audience for serious game learning, and the main intentions of the games are to compare their effectiveness with traditional methodologies and knowledge retention. The method adopted is the description of a cardiorespiratory arrest for training. The serious game proved effective for learning cardiopulmonary resuscitation.
To describe the use of the Systematized Nomenclature of Medicine - Clinical Terms (SNOMED-CT) as a model for interoperability of the nursing terminology in the national and international contexts.

This is an integrative literature review according to Cooper, which searched for articles in Portuguese, English and Spanish, published between September 2011 and November 2018 in the BVS, PubMed, SCOPUS, CINAHL, EMBASE, and Web of Science databases, ending in a sample of 15 articles.

The SNOMED-CT is a multi-professional nomenclature used by nursing in different care contexts, being associated with other standardized languages of the discipline, such as ICNP®, NANDA-I, and the Omaha System.

This review has shown that the use of SNOMED- CT is incipient in the national context, justifying the need to develop studies aimed at mapping the interoperability of existing systems of standardized language, especially NANDA-I, ICNP and Omaha System, in order to adapt the implementation of SNOMED-CT.
This review has shown that the use of SNOMED- CT is incipient in the national context, justifying the need to develop studies aimed at mapping the interoperability of existing systems of standardized language, especially NANDA-I, ICNP and Omaha System, in order to adapt the implementation of SNOMED-CT.
Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania.

Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensis.

Lulo cell protein extracts were analysed through a combination of two-dimensional gel electrophoresis and mass spectrometry and resulting spots were further investigated in silico to identify proteins using Mascot search and, afterwards, resulting sequences were applied for analysis with VectorBase.

Sixty-four spots were identified showing similarities to other proteins registered in the databases and could be classified according to their biological function, such as ion-binding proteins (23%), proteases (14%), cytoskeletal proteins (11%) and interactive membrane proteins (9.5%). Effects of interaction with L. (V.) braziliensis with the expression of three genes (enolase, tubulin and vacuolar transport protein) were observed after an eight-hour timeframe and compared to culture without parasites, and demonstrated the impact of parasite interaction with the expression of the following genes LLOJ000219 (1.
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