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Things of the Heart: Case-Crossover Evaluation involving Myocardial Infarction on Romantic days celebration.
The accurate measurement of heavy metal ions is essential for human health and environmental protection. Here, we report the design of a simple and convenient bimodal strategy for signal-on, label-free lead ion detection in environmental samples based on two-dimensional metal-organic framework (2D-MOF) nanosheets. 2D-MOFs have different affinities toward guanine-rich DNA (ssGDNA) and the G-quadruplex, allowing these structures to be distinguished. The nanosheets were also used as quenchers for fluorescent lead ion detection. Using lead ions to induce G-quadruplex formation from ssGDNA, a simple fluorescence resonance energy transfer (FRET) strategy was developed for lead ion detection; the detection limit was 3.3 nM. Based on changes in the GDNA configuration, the FRET system was converted into an electrochemical sensor for lead ion assays using an electrode modified with the 2D-MOF nanosheets. Electrochemical impedance spectroscopy showed a high sensitivity and a low limit of detection (i.e., 8.7 pM) of the electrode. The adaptability of the bimodal mechanism was verified through the successful detection of lead ions in tap water and fertilizer samples, and the method accuracy was demonstrated through inductively coupled plasma analysis. The developed bimodal device is cost-effective, highly sensitive, and allows for convenient operation, thereby rendering it a promising and reliable system for the detection of lead ions in environmental samples.A versatile flow analyzer that extended the features of reverse flow injection analysis (rFIA) was developed in this study and named reverse flow dual-injection analysis (rFDIA). Compared with typical rFIA, the analyzer requires less reagent and is more environmentally friendly, which has two injection valves and two reagent loops for the accurate and successive injection of two reagents. With a 2-m long liquid waveguide capillary cell (LWCC) and a spectrophotometer, the analyzer was applied to underway determination of dissolved iron redox species in estuarine and coastal waters. Detection limits of 0.18 and 0.20 nmol L-1 were achieved for Fe(II) and Fe(II + III), respectively and a linear dynamic range of 0.5-450 nmol L-1 was obtained for both Fe(II) and Fe(II + III). The sample throughput for the simultaneous measurement of Fe(II) and Fe(II + III) was 12 h-1, and each analysis consumed only 8 mL sample, 520 μL ferrozine solution, and 260 μL ascorbic acid solution. The analyzer was also used to measure nanomolar amounts of soluble reactive phosphorus (SRP) in seawater. The detection limit and the linear dynamic range for the SRP assay were 0.5 nmol L-1 and 1.5-850 nmol L-1. For SRP determination, the sample throughput was 20 h-1, and each analysis required 9 mL of sample, 130 μL of mixed reagent solution and 260 μL of ascorbic acid. The analytical results were reproducible, with a relative standard deviation of 1.4% (2.5 nmol L-1, n = 10), 2.1% (2.5 nmol L-1, n = 10), and 2.1% (10 nmol L-1, n = 11) for Fe(II), Fe(II + III), and SRP, respectively.Ciguatera food poisoning affects consumer health and fisheries' economies worldwide in tropical zones, and specifically in the Pacific area. The wide variety of ciguatoxins bio-accumulated in fish or shellfish responsible for this neurological illness are produced by marine dinoflagellates of the genus Gambierdiscus and bio-transformed through the food web. The evaluation of the contents of ciguatoxins in strains of Gambierdiscus relies on the availability of standards and on the development of sensitive and specific tools to detect them. There is a need for sensitive methods for the analysis of pacific ciguatoxins with high resolution mass spectrometry to ensure unequivocal identification of all congeners. We have applied a fractional factorial design of experiment 2^8-3 for the screening of the significance of eight parameters potentially influencing ionization and ion transmission and their interactions to evaluate the behavior of sodium adducts, protonated molecules and first water losses of CTX4A/B, CTX3B/C, 2-OH-CTX3C and 44-methylgambierone on a Q-TOF equipment. The four parameters that allowed to significantly increase the peak areas of ciguatoxins and gambierones (up to a factor ten) were the capillary voltage, the sheath gas temperature, the ion funnel low pressure voltage and the ion funnel exit voltage. The optimized method was applied to revisit the toxin profile of G. polynesiensis (strain TB92) with a confirmation of the presence of M-seco-CTX4A only putatively reported so far and the detection of an isomer of CTX4A. The improvement in toxin detection also allowed to obtain informative high resolution targeted MS/MS spectra revealing high similarity in fragmentation patterns between putative isomer (4) of CTX3C, 2-OH-CTX3C and CTX3B on one side and between CTX4A, M-seco-CTX4A and the putative isomer on the other side, suggesting a relation of constitutional isomerism between them for both isomers.The utilization of pesticides has been increased in recent years due to population growth and increasing urbanization. The constant use of pesticides has resulted in contamination of the environment and agricultural products with serious human health concerns associated with their use. Therefore, detection and quantification of pesticides by sensitive and selective methods is highly required in food safety management. Traditional detection methods cannot realize highly sensitive, selective and on-site detection, which limits their application. (S)2Hydroxysuccinicacid (Bio)sensors and (bio)assays are emerging tools with unique properties such as rapid, sensitive, efficient and portable detection. Among them, enzyme-based biosensors have been widely developed and some have even been commercialized. However, they suffer from some limitations such as instability and low reproducibility that originate from the nature of enzyme. Non-enzymatic (bio)sensors overcome the current limitations of enzyme-based detection methods and provide great potential for efficient, highly sensitive and low-cost detection assays using smart and miniaturized devices. In this study, we provide an overview of recent advances and new trends in optical and electrochemical non-enzymatic (bio)sensors for the detection of pesticides by focusing on antibody, aptamer and molecularly imprinted polymer (MIP) as recognition elements. Performance, advantages and drawbacks of the developed (bio)sensors are discussed well. The main advantage these recognition elements is their stability over an extended period of time compared to the enzymes. Furthermore, the combination of nanomaterials in these (bio)sensors can significantly improve their performance.
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