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Post-Transplant Maternity as well as Birth control.
BACKGROUND We aimed to explore the predictive value of radiomics signature for the recurrence-free survival (RFS) in patients with resected stage I non-small-cell lung cancer (NSCLC). METHODS From January 2009 to December 2011, patients with resected stage I NSCLC were divided into sub-solid and pure-solid groups according to presence of ground glass opacity (GGO) in computed tomography (CT). A total of 107 extracted radiomics features were reduced to 8 features by using LASSO-Cox analysis to develop a radiomics signature for RFS prediction. Univariate and multivariate survival analyses were applied to identify independent prognostic variables, the Harrell concordance index (C-index) was measured to assess their prediction performance. RESULTS Our study included 378 patients with a median follow-up time of 63.2 months. The radiomics signature could stratify all patients into high-risk (180/378) and low-risk group (198/378) with different RFS (p less then 0.001). In the sub-solid group (n=115), 3 patients who occurred relapse were categorized into high-risk group by the radiomics signature. In pure-solid group, patients with low-risk (141/263) had a better outcome than those with high risk (122/263) (p less then 0.001). Multivariate analyses revealed that the histology (p less then 0.001), and the developed radiomics signature (p less then 0.001) remained independent prognostic factors for RFS. CONCLUSION Radiomics signature may be an independent imaging biomarker for predicting the survival, which may guide for personalizing treatment option in patients with stage I NSCLC. BACKGROUND The utilization of multi-organ transplantation in cardiac transplantation has steadily increased over the past several years. We sought to characterize the trends and outcomes in simultaneous heart and other organ transplantation compared to heart transplantation alone. METHODS The UNOS database was queried for all adult patients (age ≥ 18) who underwent isolated heart transplantation or simultaneous heart-lung or heart-kidney transplantation from 1987-2016. Patients were stratified into three equal time intervals. Demographics and postoperative outcomes were compared. Furosemide in vitro RESULTS A total of 58,060 patients were identified with a distribution based on era. Dual organ recipients had more factors associated with increased operative risk including higher rates of diabetes, pulmonary hypertension, intensive care unit (ICU) admissions, and dialysis prior to transplantation. Heart-lung and heart-kidney recipients had decreased one-year survival compared to isolated heart recipients from 2007-2016. However, heart-kidney recipients had significantly increased five-year post-transplantation survival compared to isolated heart recipients with impaired renal function. For isolated heart transplants and heart-lung transplants, five-year survival rates improved over time, while five-year survival for heart-kidney recipients did not improve with time. CONCLUSIONS We found a significantly increased five-year survival rate for heart-kidney transplant recipients compared to isolated heart transplant recipients with renal impairment. Lack of improvement in five-year post-operative outcomes for heart-kidney recipients in the setting of higher risk pre-transplant clinical characteristics suggests decreased selectivity regarding heart-kidney recipients. Continued scrutiny and evaluation of post-operative outcomes are required to ensure just and appropriate utilization of organs. A lateral flow assay using DNA aptamer-based sensing for the detection of dopamine in urine is reported. Dopamine duplex aptamers (hybridized sensor with capture probe) are conjugated to 40-nm Au nanoparticles (AuNPs) with 20T linkers. The detection method is based on the dissociation of the duplex aptamer in the presence of dopamine, with the sensor part undergoing conformational changes and being released from the capture part. Hybridization between the complementary DNA in the test line and the conjugated AuNP-capture DNA produces a red band, whose intensity is related to the dopamine concentration. The minimum detectable concentration obtained by ImageJ analysis was less then 10 ng/mL (65.2 nM), while the visual limit of detection is estimated to be ~50 ng/mL (normal range of dopamine in urine of 52-480 ng/mL or 0.3-3.13 μM). No cross reactivity to other stress biomarkers in urine was confirmed. These results indicate that this robust and user-friendly point-of-care biosensor has significant potential for providing a cost-effective alternative for dopamine detection in urine. Sample types such as those from reproductive systems often yield scarce material, which limits RT-qPCR analysis to only a few targets. Recently developed high-throughput systems can potentially change this scenario, however, the nanoliter scale of such platforms requires extra processing, e.g., preamplification, which needs to be defined through observation and experience. In order to establish best practices in high-throughput PCR approaches using samples from reproductive systems, we evaluated the Biomark™ HD performance using 11 different sample types from the bovine reproductive system blastocyst (single/pool), oocyte (pool), cumulus, granulosa, and theca cells, oviduct tissue, fetal ovary, testicle (adult/fetal), and uterine horn. We observed that the preamplification step is not just reliable, but mandatory. Our results indicated that 14-preamplification cycles associated to 5- and 7-fold-dilution is the best approach for those samples. Additionally, the Biomark™ HD system has a high intra and inter reproducibility, therefore its performance in duplicate is unnecessary for the ΔCq analysis, taking in consideration the cutoff value 4 less then Cq less then 22. In summary, this high-throughput approach is a reliable and excellent tool for studying the bovine reproductive system, especially using quantitatively-limited samples, as a larger number of target genes can be assessed from a very low amount of starting material. Aptamers are a class of short artificial single-stranded oligo(deoxy) nucleotides that can bind to different targets, which generated by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Due to excellent selectivity and high affinity to targets, aptamers hold considerable potential as molecular probe in diverse applications ranging from ensuring food safety, monitoring environment, disease diagnosis to therapy. This review highlights recent development and challenges about aptamers screened by Cell-SELEX, and its application about cancer diagnostics and therapeutics. Advances about some operation methods such as operation method and culture method in aptamers selection procedure were summarized in this paper. Some common challenges and technological difficulties such as nonspecific binding and biostability were discussed. Up to now, the recent endeavors about cancer diagnostic and therapeutic applications of aptamers are summarized and expatiated. Most of aptamers screened by Cell-SELEX took tumor cells as target cells, and such aptamers have been assembled to various aptasensor for cancer diagnosis.
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