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This study aimed at the assessment of the impact of various culinary processes on the fate of chromium (Cr) species (Cr(III) and Cr(VI)) in infant formula milk, semi-skimmed milk and bovine meat samples. The cooking procedures were boiling at 70°C/100°C (milk samples) and frying without and with oil (95°C and 120°C) (bovine meat). The levels of Cr(III) and Cr(VI) in raw and cooked samples were determined by high-performance liquid chromatography (HPLC) coupled to inductively coupled plasma mass spectrometry (ICP-MS) using double spike species-specific-isotope dilution (SS-ID). The species were extracted by sequential complexation of Cr(III) with ethylenediaminetetraacetic acid and of Cr(VI) with 1,5-diphenylcarbazide in the same analytical run by heating at 70°C for 50 min. Anion exchange chromatography using a Dionex IonPac™ AG7 column and a mobile phase consisting of 10 mM HNO3, 2.5% MeOH and 30 mM EDTA at pH 2 was employed for species separation. The quantification limits were 0.013 and 0.049 µg kg-1 for Cr(III) and Cr(VI), respectively. ANOVA test used to compare the mean Cr species concentrations showed no significant differences between raw and cooked samples. The results obtained in the present study show that oxidation of Cr(III) to Cr(VI) does not occur during thermal cooking of milk and bovine meat samples. A selection of 10 samples of each type were analysed in terms of total Cr (Crtotal) as well as speciation (Cr(III) and Cr(VI)). Cr(VI) was not quantified in any of these samples, whereas Cr(III) levels ranged from 0.22 (infant formula milk) up to 80 µg kg-1 (chorizo sausage). Additionally, Cr(III) and Crtotal levels were comparable hence demonstrating that in the samples analysed in this study, Cr is found exclusively as Cr(III) species.This study assessed the validity of 5-m (TG5) and 10-m (TG10) split times measured with timing gates to estimate maximum sprint speed (MSS) against a criterion measure radar gun (RG) during the maximum velocity phase of a 30-m sprint. Nineteen amateur rugby players performed two 30-m sprints. The timing gates were placed at the starting line and at 5-, 10-, 20-, 25- and 30-m. In addition, a RG was used to measure instantaneous velocity. Both trials per participant were used selected as references. MSS obtained from TG10, TG5 and RG showed high intraclass correlation coefficients (0.971-0.978), low coefficients of variation (1.14-1.70%) and smallest detectable changes ( 0.926, p less then 0.001). In conclusion, MSS obtained from TG5, TG10 and RG presented good intra-session reliability. selleck chemical However, practitioners should be aware that substantial differences exist between TG10 and RG. For the assessment of MSS in team-sport athletes, it is recommended the use of TG5 for more accurate estimations when a gold standard criterion is not available.Objective. Reducing sedentary behaviours at work is imperative. Before effective strategies can be developed there is a need to understand profiles of activity within particular roles and organizations. This study aimed to determine activity profiles of staff by job title at a UK university. Methods. Three-hundred and seventeen participants completed the international physical activity questionnaire - short form to determine physical activity profiles. Fifty-one participants also wore a wrist-worn GENEActiv accelerometer for 7 days and completed a self-report diary denoting work and leisure hours. Results. Twenty-one per cent of respondents were categorized as inactive and achieved 298 ± 178 metabolic equivalent minutes (MET-min)/week. Those in administrative roles were most sedentary (501 ± 161 min/day). Accelerometer data highlighted that sedentary time was identical between job roles (pooled mean 8746 ± 823 counts) and equated to 84 ± 9% of total time. During working hours, management, professional and specialist job roles had the highest level of sedentary time (2066 ± 416 counts). Conclusion. Time spent undertaking sedentary activities during working hours contributes to reduced overall activity and can impede productivity, performance and health. Interventions encouraging regular movement and preventing sedentary behaviours at work are therefore required.The back handspring is one of the most commonly performed skills on the balance beam in women's gymnastics. Despite this, quantitative research on the beam has often been overlooked. This study aimed to investigate the kinetic and kinematic variables during the performance of two back handspring techniques on the beam the back handspring with two footed landing and the back handspring step-out. A modified balance beam was fixed to a force plate with an isolated mat for landing to allow analysis of the take-off and hand contact phases. Kinetic and kinematic data were recorded for twelve gymnasts performing both techniques. No statistically significant differences between techniques were found during take-off. However, average peak vertical and horizontal ground reaction forces (4.1 bodyweights ± 1.1 BW, and 0.7 BW ± 0.2 BW, respectively) were higher and time to peak force shorter during the hand contact phase for the two footed variant. A more vertical trajectory, along with a greater hand contact ground reaction force were found in comparison to the back handspring performed on the floor. These results highlight the need for more specific investigation to understand the factors which could contribute to reducing the load faced during balance beam performance.This study compares the effects of different neck flexion angles on neck gravitational moment and muscle activity of users that stand and operate a smartphone. Thirty-two healthy young adult smartphone users performed texting tasks for three minutes at four different neck flexion angles (0°, 15°, 30°, and 45°) while standing. Neck gravitational moment and cervical erector spinae (CES) and upper trapezius (UT) activity were investigated. When the neck flexion angle increased, the gravitational moment of the neck increased significantly. The muscle activity of CES significantly increased when the neck flexion angle increased, whereas that of UT decreased. The lowest gravitational moment of the neck at 0° flexion was consistent with the lowest CES muscle activity and the lowest neck discomfort score. In conclusion, for texting while standing, adults should maintain their neck posture at 0° flexion to reduce the gravitational force acting on the cervical spine and alleviate neck discomfort. Practitioner Summary During smartphone use when standing, excessive neck flexion (30° and 45° flexion) should be avoided.
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