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Background Interactive echocardiography translation is an efficient educational function to master cardiac anatomy. It strengthens the student's understanding by pixel-level translation between echocardiography and theoretically sketch images. Previous research studies split it into two aspects of image segmentation and synthesis. This split makes it hard to achieve pixel-level corresponding translation. Besides, it is also challenging to leverage deep-learning-based methods in each phase where a handful of annotations are available. Methods To address interactive translation with limited annotations, we present a two-step transfer learning approach. Firstly, we train two independent parent networks, the ultrasound to sketch (U2S) parent network and the sketch to ultrasound (S2U) parent network. U2S translation is similar to a segmentation task with sector boundary inference. Therefore, the U2S parent network is trained with the U-Net network on the public segmentation dataset of VOC2012. S2U aims at recovering ultrasound texture. So, the S2U parent network is decoder networks that generate ultrasound data from random input. After pretraining the parent networks, an encoder network is attached to the S2U parent network to translate ultrasound images into sketch images. We jointly transfer learning U2S and S2U within the CGAN framework. Results and conclusion. Quantitative and qualitative contrast from 1-shot, 5-shot, and 10-shot transfer learning show the effectiveness of the proposed algorithm. The interactive translation is achieved with few-shot transfer learning. Thus, the development of new applications from scratch is accelerated. Our few-shot transfer learning has great potential in the biomedical computer-aided image translation field, where annotation data are extremely precious. Copyright © 2020 Long Teng et al.Background Brassica napus is an important oilseed crop that offers a considerable amount of biomass for global vegetable oil production. The establishment of an efficient genetic transformation system with a convenient transgenic-positive screening method is of great importance for gene functional analysis and molecular breeding. However, to our knowledge, there are few of the aforementioned systems available for efficient application in B. napus. Results Based on the well-established genetic transformation system in B. napus, five vectors carrying the red fluorescence protein encoding gene from Discosoma sp. (DsRed) were constructed and integrated into rapeseed via Agrobacterium-mediated hypocotyl transformation. An average of 59.1% tissues were marked with red fluorescence by the visual screening method in tissue culture medium, 96.1% of which, on average, were amplified with the objective genes from eight different rapeseed varieties. In addition, the final transgenic-positive efficiency of the rooted plantlets reached up to 90.7% from red fluorescence marked tissues, which was much higher than that in previous reports. Additionally, visual screening could be applicable to seedlings via integration of DsRed, including seed coats, roots, hypocotyls and cotyledons during seed germination. These results indicate that the highly efficient genetic transformation system combined with the transgenic-positive visual screening method helps to conveniently and efficiently obtain transgenic-positive rapeseed plantlets. Conclusion A rapid, convenient and highly efficient method was developed to obtain transgenic plants, which can help to obtain the largest proportion of transgene-positive regenerated plantlets, thereby avoiding a long period of plant regeneration. The results of this study will benefit gene functional studies especially in high-throughput molecular biology research. © The Author(s) 2020.We present a study and reflection about the role and use of smartphone technology for a large-scale musical performance involving audience participation. We evaluated a full design and development process from initial ideation to a final performance concept. We found that the smartphone became the design tool, the technical device and the musical instrument at the same time. As a technical device that uses ultrasound communication as interaction technique, the smartphone became inspirational for the artist's creative work. In aiming to support the artist, we observed pervasive importance of retaining artistic control to realise artistic intent. This concerns the co-design process and the resulting concept of audience participation and supports recommendations for such participatory work. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.When playing single-reed woodwind instruments, players can modulate the spectral content of the airflow in their vocal tract, upstream of the vibrating reed. In an empirical study with professional clarinettists ( N p = 11 ), blowing pressure and mouthpiece pressure were measured during the performance of Clarinet Concerto excerpts. By comparing mouth pressure and mouthpiece pressure signals in the time domain, a method to detect instances of vocal tract adjustments was established. Results showed that players tuned their vocal tract in both clarion and altissimo registers. Furthermore, the analysis revealed that vocal tract adjustments support shorter attack transients and help to avoid lower bore resonances. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.In the present text, I will reflect upon the composition of works that use virtual 3D environments in which the performers are represented as interacting avatars. In particular, I will explain the musical possibilities offered by this work with virtual environments, detailing the effects that the design of virtual topologies has on the interactive sound sources and sound events contained in these topologies. I will focus particularly upon how performers can musically influence said effects by navigating with the avatars acting within the topology. © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.Background Obesity is a severe public health threat worldwide. Emerging evidence suggests that gut microbiota dysbiosis is closely associated with obesity and its related metabolic complications. The aim of the present study is to investigate the effects of polysaccharide extracted from WuGuChong (PEW) on high-fat diet (HFD)-induced obesity, and the potential mechanisms involving modulation of the gut microbiota composition. Methods Mice were fed a normal chow diet and a high-fat diet with or without PEW (300 mg/kg/day) by oral gavage for 8 weeks. Body weight, obesity-related metabolic disorders, and gut microbiota were examined at the end of the experiment. Vorinostat Results PEW supplementation reduces body weight, adipose hypertrophy, liver steatosis, insulin resistance and systemic inflammation in HFD-fed mice, as well as maintains intestinal epithelium integrity. High-throughput 16S rRNA analysis demonstrates that PEW supplementation alters the composition of gut microbiota. The Firmicutes to Bacteroidetes ratio and the relative abundance of Proteobacteria are increased in HFD-fed mice, which are reversed by PEW supplementation to approximately the control levels.
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