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The International Maritime Organization and U.S. Coast Guard have implemented regulations to reduce introductions of non-indigenous species via ballast water (BW). For phytoplankton, regulations limit discharges to less then 10 live/viable cells mL-1 (size 10-50 μm), ignoring other size fractions. Additionally, challenge conditions of 100 (shipboard) and 1000 (land-based) cells mL-1 are required in BW management system certification testing. How these requirements correspond to natural phytoplankton populations is poorly resolved. We analyzed phytoplankton samples from 31 major ports to evaluate a) how natural communities compare to challenge requirements and b) abundances of unregulated size fractions (i.e., less then 10 and ≥50 μm). None of the ports met land-based challenge conditions, and only 32% met requirements for shipboard testing. Approximately 71% of organisms ≥50 μm were centric diatoms, also unregulated by current protocols. This study demonstrates that current regulations do not consider natural phytoplankton populations, limiting control efforts for potentially harmful non-indigenous species.Microplastic (MP) pollution has become a global concern. We aim to quantify the extent of MP pollution in the coastal ecosystem of the Port Blair Bay, A&N Islands. Water, sediment, zooplankton, finfish, and shellfish samples were collected from the Port Blair Bay and analyzed for the presence of MP. Average concentrations of MP in water, sediment, zooplankton, finfish, and shellfishes were found to be 0.93 ± 0.59 particles per m3, 45.17 ± 25.23 particles per kilogram, 0.12 ± 0.07 pieces per zooplankter and 10.65 ± 7.83 particles per specimen, respectively. High amount of MP retention was observed in the zooplankton community. Maximum MP ingestion was observed in adult Carangoides malabaricus. Fiber was most abundant in water, sediment, and fish samples, followed by fragment and pellet. However, fragments were predominant in zooplankton. Nylon, acrylic, and ionomer surlyn were most abundant polymer types in the bay environment. These results demand further attention to combat plastic pollution in the coastal ecosystem.To explore the geographical variations in the nosZ-denitrifier community and the underlying influential factors, surface sediments were collected from six mangroves across China, including Yunxiao (YX), Futian (FT), Fangchenggang (FCG), Zhanjiang (ZJ), Dongzhaigang (DZG), and Dongfang (DF). The nosZ gene abundance in mangrove sediments were 1.60 × 105-1.17 × 106 copies g-1 dry sediment, with a higher density in Avicennia marina forest than the mudflat. Denitrifier community richness and diversity increased with decreasing latitude based on the Chao1 richness and Shannon diversity index, with the highest diversity being observed in the DF mangrove. The denitrifier communities could be classified into three groups including south DF mangrove, middle FCG, ZJ and DZG mangroves, and north YX and FT mangroves based on HCA and PCoA analysis. The nosZ OTUs could be divided into seven distinct clusters with different proportionality characteristics among mangroves. Environmental factors (TN, TOC, and salinity) collectively shape denitrifier communities in mangrove sediments.The 3D dispersion of marine litter (ML) over the Mediterranean basin has been simulated using the velocity fields from a high resolution circulation model as base to run a 3D Lagrangian model. Three simulations have been performed to mimic the evolution of ML with density lower, similar, or higher than seawater. In all cases a realistic distribution of ML sources was used. Our results show that the accumulation/dispersion areas of the floating and buoyancy neutral particles are practically the same, although the latter are distributed in the water column, 80% of them found in the photic layer (average depth of 35m). Regarding to the densest particles, they rapidly sink and reach the seafloor close to their source. The regions of higher temporal variability mostly coincide with the ML accumulation regions. Weak seasonal variability occurs at a sub-basin scale as a result of the particles redistribution induced by the seasonal variability of the current field.Biogeochemical monitoring coupled with multi-biomarker approach were performed for the assessment of marine environment, using clam Ruditapes philippinarum and scallop Chlamys farreri to indicate contamination status in sediments and seawater respectively. The bivalves were collected from three stations, Jiaozhou Bay, Rushan Bay and Laizhou Bay, of Shandong coastal area. A series of contaminants (PAHs and TBBPA) and biomarkers (AhR, EROD, GST, SOD, GPx, CAT, DNA damage) were measured. this website Multi-biomarker pollution index (MPI) and integrated biomarker response (IBR) were carried out to evaluate contamination status and both indexes showed that Rushan Bay was most polluted, where the pollution level of sediments reached "highly polluted" in August, followed by Jiaozhou Bay and Rushan Bay which reached "lightly polluted". The correlation of IBR values with contaminants' concentrations was verified through the Pearson correlation coefficient (p less then 0.05), consolidating this scientific assessment method for marine environment.The uptake and discharge of bivalve larvae through ballast water operations is a highly viable mechanism for transfer of shellfish. In this paper, we investigate the effects of common water treatment processes on the planktotrophic larvae of the two invasive species of shellfish, Mytilopsis sallei and Mytella strigata. The study found that common water treatment processes used in many ballast water treatment systems were effective for the removal of bivalve larvae, although later stages of larval forms required more effort to remove.Kyiv is Ukraine's capital and largest city. Home to 3 million people, this area has a rich history of agriculture and industry. The Dnieper River is Ukraine's largest river and it passes through the center of Kyiv. Little information on emerging and legacy compounds or their toxicity in the Dnieper River exists. For this investigation, water was sampled for PAHs, PCBs, metals and emerging contaminants including pharmaceuticals and personal care products. The effects of surface waters in the Dnieper were evaluated using the Ames, chronic and acute daphnia, and a ciliate (Colpoda stennii) assays. Concentrations of legacy and emerging contaminants were found in seven stations near the municipal water treatment plant (MWTP) and receiving waters. The MWTP appeared to remove some of the emerging contaminants, however the legacy compounds (PCBs and PAHs) were not affected by the MWTP and appeared to be more wide-spread indicating a number of sources to the Dnieper River. Acute and chronic toxicity were associated with the influent and effluent of the MWTP, however mutagenicity was noted in surface waters throughout the Dnieper River including upstream of the MWTP.
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