Notes

FEMA GRAS assessment of all-natural flavoring buildings: Eucalyptus gas along with other cyclic ether-containing flavoring elements.
CircRNA, a type of circular RNA, has recently been shown to be a potential target for osteoarthritis (OA). Circular RNA HIPK3 (CircHIPK3) is reported to be abnormally expressed in various disease tissues and affects the occurrence and development of the disease. However, the role and underlying mechanism of CircRNA HIPK3 in osteoarthritis are still unclear. The purpose of this study is to explore the effect of CircRNA HIPK3 on osteoarthritis and analyze its regulatory mechanism.

We took human OA tissues, normal knee cartilage, human OA chondrocytes and normal chondrocytes as the research objects. Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect CircHIPK3 expression level, its target gene miRNA 124 (miR-124) and downstream target molecule SOX8. Flow cytometry analysis was applied to discover the apoptosis of CircHIPK3 and miR-124 on OA cartilage in different transfection situations. Moreover, Western blot and RT-qPCR were used to detect the expression of caspase-3 in OA chondroular mechanism of CircHIPK3 in present study is expected to provide new ideas for the treatment of osteoarthritis.
Our results demonstrated that CircHIPK3 was significantly upregulated in OA cartilage tissue and cells. Low expression of CircHIPK3 promoted the apoptosis of OA chondrocytes by promoting miR-124 to suppress SOX8 expression. The molecular mechanism of CircHIPK3 in present study is expected to provide new ideas for the treatment of osteoarthritis.
Osteoarthritis is a degenerative disease characterized by degeneration of articular cartilage, but the current mechanism is unclear. Circular RNA (circRNA) plays a significant role in a series of biological processes related to osteoarthritis, but its mechanism remains unclear. The purpose of this study was to investigate the role of the circTMBIM6/miR-27a/MMP13 axis in osteoarthritis.

The expression levels of circTMBIM6, miR-27a and MMP13 in cartilage of osteoarthritis patients and normal human cartilage were detected by reverse transcription polymerase chain reaction (RT-PCR). Osteoarthritis cell model was induced by IL-1β and TNF-α, and the expression changes of circTMBIM6, miR-27a and MMP13 in the in vitro model were detected. In addition, the in vitro regulation of circTMBIM6 and miR-27a in osteoarthritis was verified by transfection of circTMBIM6 and miR-27a plasmids, and the regulation of miR-27a on MMP13 was also verified. The dimethylmethylene blue (DMMB) method was used to analyze the secretion and formation of soluble glycosaminoglycan sulfate (sGAG), and the effects of circTMBIM6 and miR-27a chondrocytes were evaluated.

The expression levels of circTMBIM6 and MMP13 in cartilage tissue of patients with osteoarthritis were higher than that of normal group, while the expression level of miR-195 in cartilage tissue of patients with osteoarthritis was lower. After IL-1β and TNF-α treatment, the expression of circTMBIM6 and MMP13 in chondrocytes increased, while the expression of miR-27a decreased. CircTMBIM6 overexpression reduced miR-27a expression but increased MMP13 expression. The circTMBIM6 gene knockout showed the opposite effect.

CircTMBIM6 promotes osteoarthritis-induced chondrocyte extracellular matrix degradation via miR-27a/MMP13 axis.
CircTMBIM6 promotes osteoarthritis-induced chondrocyte extracellular matrix degradation via miR-27a/MMP13 axis.The article "Knockdown of microRNA-181a inhibits osteosarcoma cells growth and invasion through triggering NLRP3-dependent pyroptosis, by B.-G. Tian, Z. Hua, Z.-J. Wang, J. Li, published in Eur Rev Med Pharmacol Sci 2020; 24 (3) 1030-1040-DOI 10.26355/eurrev_202002_20153-PMID 32096182" has been withdrawn from the authors. The Publisher apologizes for any inconvenience this may cause. https//www.europeanreview.org/article/20153.The article "LncRNA H19 inhibitor represses synovial cell proliferation and apoptosis in rats with rheumatoid arthritis via Notch signaling pathway, by L.-Q. Zhi, Q. Zhong, J.-B. Ma, L. Xiao, S.-X. Yao, X. Wang, published in Eur Rev Med Pharmacol Sci 2020; 24 (8) 4088-4094-DOI 10.26355/eurrev_202004_20985-PMID 32373945" has been withdrawn from the authors. The Publisher apologizes for any inconvenience this may cause. https//www.europeanreview.org/article/20985.The article "Overexpression of miR-150 alleviates mechanical stress-accelerated the apoptosis of chondrocytes via targeting GRP94, by Z.-Q. Zhang, C.-S. Wang, P. Yang, K.-Z. Wang, published in Eur Rev Med Pharmacol Sci 2019; 23 (18) 7775-7785-DOI 10.26355/eurrev_201909_18987-PMID 31599403" has been withdrawn from the authors. The Publisher apologizes for any inconvenience this may cause. https//www.europeanreview.org/article/18987.The article "MiR-195 inhibits myocardial fibrosis in hypertensive rats by regulating TGFβ1-Smad3 signaling pathway, by Q. Xu, X.-X. Cetuximab Lin, P. Liu, W. Zhang, K. Tang, Y.-S. Zhai, L.-J. Liu, W.-Y. Mei, published in Eur Rev Med Pharmacol Sci 2019; 23 (18) 8087-8094-DOI 10.26355/eurrev_201909_19026-PMID 31599435" has been withdrawn from the authors. The Publisher apologizes for any inconvenience this may cause. https//www.europeanreview.org/article/19026.Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "Long noncoding RNA LINP1 promoted proliferation and invasion of ovarian cancer via inhibiting KLF6, by Y. Li, C.-Z. Hou, Y.-L. Dong, L. Zhu, H. Xu, published in Eur Rev Med Pharmacol Sci 2020; 24 (1) 36-42-DOI 10.26355/eurrev_202001_19893-PMID 31957816" has been withdrawn. The Publisher apologizes for any inconvenience this may cause. https//www.europeanreview.org/article/19893.Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "Downregulation of lncRNA SNHG7 inhibits proliferation and invasion of nasopharyngeal carcinoma cells through repressing ROCK1, by L. Wang, T. Xu, X. Cui, M. Han, L.-H. Zhou, Z.-X. Wei, Z.-J. Xu, Y. Jiang, published in Eur Rev Med Pharmacol Sci 2019; 23 (14) 6186-6193-DOI 10.26355/eurrev_201907_18432-PMID 31364118" has been withdrawn. The Publisher apologizes for any inconvenience this may cause. https//www.europeanreview.org/article/18432.
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