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Connection between ethyl acetate extract involving Kaempferia parviflora in brownish adipose tissue.
Taylor, RJ, Sanders, D, Myers, T, and Akubat, I. Reliability and validity of integrated external and internal load ratios as measures of fitness in academy rugby union players. J Strength Cond Res 34(6) 1723-1730, 2020-This study aimed to assess the relationships between integrated ratios of external and internal load measures and aerobic fitness in 3 different training exercise protocols. Twelve academy rugby union players (aged; 17.6 ± 0.44 years, height; 179.4 ± 6.3 cm, body mass 83.3 ± 9.7 kg) performed a lactate threshold/V[Combining Dot Above]O2max test (LT/V[Combining Dot Above]O2max) followed by 3 exercise protocols; 760 m continuous shuttle running (20 m shuttle run at 9 km·h), sprint interval training (SIT 6 × 6 seconds sprint with 54 seconds recovery), and a small-sided game (SSG 6 vs. 6, 10 minutes on a 39 × 51 m pitch) on 2 occasions. A LT/V[Combining Dot Above]O2max test was used to determine velocity at lactate threshold (vLT) and at the onset of blood lactate accumulation (vOBLA), maximal oxygen uptake (V[Combining Dot Above]O2max) and the heart rate-blood lactate profile for the calculation of internal load (individualized training impulse or iTRIMP). The total distance (TD), PlayerLoad, metabolic power (MP), high-speed distance >15 km·h (HSD), very high-speed distance >18 km·h (VHSD) and individualized high-speed distance based on each player's vOBLA (iHSD) for the 3 exercise protocols were measured using Micro Electro Mechanical Systems/Global Positioning System technology. Bayesian analysis was used to assess the ratios validity and reliability. Ratios demonstrated large-to-very large associations with vOBLA (Rho = 0.64-0.76), vLT (Rho = 0.63-0.71). Reliability of the ratios ranged from 7.06 to 36.28% (coefficient of variation [CV]%). The results suggest that integrated load ratios from the SIT and SSG protocols provide a measure which is reliable with large-to-very large associations to submaximal aerobic fitness in rugby union.Bagley, JR, Burghardt, KJ, McManus, R, Howlett, B, Costa, PB, Coburn, JW, Arevalo, JA, Malek, MH, and Galpin, AJ. Epigenetic responses to acute resistance exercise in trained vs. sedentary men. J Strength Cond Res 34(6) 1574-1580, 2020-Acute resistance exercise (RE) alters DNA methylation, an epigenetic process that influences gene expression and regulates skeletal muscle adaptation. This aspect of cellular remodeling is poorly understood, especially in resistance-trained (RT) individuals. The study purpose was to examine DNA methylation in response to acute RE in RT and sedentary (SED) young men, specifically targeting genes responsible for metabolic, inflammatory, and hypertrophic muscle adaptations. Vastus lateralis biopsies were performed before (baseline), 30 minutes after, and 4 hours after an acute RE bout (3 × 10 repetitions at 70% 1 repetition maximum [1RM] leg press and leg extension) in 11 RT (mean ± SEM age = 26.1 ± 1.0 years; body mass = 84.3 ± 0.2 kg; leg press 1RM = 412.6 ± 25.9 kg) and 8 SED (age = 22.9 ± 1.1 years; body mass = 75.6 ± 0.3 kg; leg press 1RM = 164.8 ± 22.5 kg) men. DNA methylation was analyzed through methylation sensitive high-resolution melting using real-time polymerase chain reaction. Separate 2 (group) × 3 (time) repeated-measures analyses of variance and analyses of covariance were performed to examine changes in DNA methylation for each target gene. Results showed that acute RE (a) hypomethylated LINE-1 (measure of global methylation) in RT but not SED, (b) hypermethylated metabolic genes (GPAM and SREBF2) in RT, while lowering SREBF2 methylation in SED, and (c) did not affect methylation of genes associated with inflammation (IL-6 and TNF-α) or hypertrophy (mTOR and AKT1). However, basal IL-6 and TNF-α were lower in SED compared with RT. These findings indicate the same RE stimulus can illicit different epigenetic responses in RT vs. SED men and provides a molecular mechanism underpinning the need for differential training stimuli based on subject training backgrounds.Marosek, SEH, Antharam, V, and Dowlatshahi, K. Quantitative analysis of the acetic acid content in substances used by athletes for the possible prevention and alleviation of exercise-associated muscle cramps. J Strength Cond Res 34(6) 1539-1546, 2020-Athletes regularly consume commercially available food and sports shot products, carbohydrate beverages, and water to improve their physical exertion and to possibly prevent or relieve exercise-associated muscle cramps (EAMCs)-often experienced during practice, training, or competition. Acetic acid, a component of interest within these products, has been recognized for its potential role in cramp reduction. Acetic acid is postulated to mitigate cramping by decreasing alpha motor neuron activity through oropharyngeal stimulation and inhibitory neurotransmitter production, while aiding in the role acetylcholine plays in muscle contraction and relaxation. The purpose of this research is to analytically assess the most viable sources of acetic acid from substances that athletes ingest before or when experiencing these cramps. The range of samples investigated were based on their widespread use in the athletic world dill and sweet pickle juices, yellow mustard, sweet relish, apple cider vinegar, Hot Shot, PJ Shot, PJ Sport, E-Lyte Sport, Powerade, Gatorade, Smartwater, and Propel (with electrolytes). As hypothesized, pH and enzymatic assay or spectroscopic analyses revealed that yellow mustard, sweet relish, all pickle juices, and the pickle juice products were composed of moderate amounts of acetic acid. Based on established studies resulting in EAMC relief, acetic acid consumption, and the appropriate serving size, the yellow mustard, PJ Shot, and all pickle juices would be the most practical and palatable sources of acetic acid for strength and conditioning professionals to recommend that athletes consume for the possible prevention or alleviation of muscle cramps.Campbell, PG, Stewart, IB, Sirotic, AC, and Minett, GM. check details Title The effect of overreaching on neuromuscular performance and wellness responses in Australian rules football athletes. J Strength Cond Res 34(6) 1530-1538, 2020-This study seeks to evaluate the effect of periodized fluctuations in training load on wellness and psychological questionnaires, perceived exertion, performance, and neuromuscular measures in team-sport athletes. Thirteen amateur Australian rules football athletes completed 6 weeks of periodized training, consisting of 2-week normal training (NT), intensified training (IT), and taper training (TT). Training sessions were quantified using global positioning system devices, heart-rate, and session rating of perceived exertion (sRPE), with wellness (general soreness, sleep quality/quantity, readiness to train, fatigue, stress, mood, and motivation) questionnaires collected daily. Psychological (Recovery-Stress Questionnaire for Athletes) and physical performance (countermovement jump, cycle ergometer peak power, 30-m sprint, and 2-km time trial) markers were measured after each training period.
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