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This study systematically investigated the dynamic changes in bacterial communities in roast chicken in normal and modified atmosphere packaging (MAP). The samples were stored under normal atmosphere and 40%/60% CO2 /N2 MAP conditions for 28 days at 4 °C. Changes in the number and type of microorganisms in roast chicken during storage were defined via cultural and 16S rDNA sequencing techniques. More Bacteroides, Chryseobacterium, Lactobacillus, and Acinetobacter than other bacteria were initially found in roast chicken. With normal packaging, Pseudomonas rapidly multiplied and became the main spoilage organism in roast chicken after 7 days, with a relative abundance of >90% of the entire bacterial flora. With MAP, due to the high salt content, Halomonas became the main spoilage organism in roast chicken by the middle of the storage period (14 days). Between days 21 and 28 of storage, Pseudomonas gradually became the main spoilage organism in roast chicken, but its relative abundance was much lower in MAP than in normal packaging, followed by Lachnospiraceae (NK4A136 group) and Altererythrobacter. Our research shows that the microbes in roast chicken mainly originated from the processing environment and operators. The combination of MAP with a low storage temperature could effectively improve the quality and safety of roast chicken meat. PRACTICAL APPLICATIONS This research showed the dynamic changes in the bacterial community of roast chicken stored under normal and modified atmosphere packaging (MAP). Microorganisms in roast chicken are mainly obtained from the processing environment and operators. Combining MAP with storage at low temperatures can effectively improve the quality and safety of roast chicken. © 2020 Institute of Food Technologists®.Samples of Pithophoraceae, collected in diverse freshwater and damp terrestrial habitats from tropical and subtropical China, were characterized morphologically and ultrastructurally, and their phylogenetic position determined based on nuclear ribosomal DNA sequences. Our phylogenetic analysis resolved a novel lineage of Pithophoraceae, sister to Aegagropilopsis. Based on our phylogenetic results, morphological observations and comparative rDNA ITS2 secondary structure analysis, we propose Chlorocladiella gen. nov., characterized by a well-developed system of prostrate filaments, and describe four new species, C. Mycophenolic cochlea sp. nov., C. erecta sp. nov., C. medogensis sp. nov., and C. pisformis sp. nov. Two species were found growing on damp soil, which is an unusual habitat for cladophoralean green algae, indicating that the diversity of Cladophorales in terrestrial habitats may be greater than currently recognized. This article is protected by copyright. All rights reserved.Variations in the taste quality of no-added-nitrite Chinese bacon (unsmoked) during processing were investigated using 1 H-NMR and multivariate data analysis. The results showed that 21 metabolites were dominant during processing, which involved marinating, air-drying, fermentation, and baking, including amino acids, sugars, organic acids, nucleic acids and their derivatives, and alkaloids. The contents of isoleucine, leucine, valine, alanine, acetate, glutamate, succinate, glycine, sucrose, tyrosine, and phenylalanine increased continuously throughout the process. The lactate, creatine, carnosine, betaine, taurine, hypoxanthine, and AMP contents all significantly increased after baking; the inosine content significantly increased after fermentation and then decreased; the histamine content significantly increased after air-drying and then decreased; and the histidine content decreased. Each processing treatment promoted taste formation in no-added-nitrite Chinese bacon (unsmoked), especially baking. The bakistudy determined the main taste components of no-added-nitrite Chinese bacon (unsmoked) and its formation process, which provides new insight into the production and characteristics of flavor in Chinese bacon (unsmoked). © 2020 Institute of Food Technologists®.Thermal processing of pulse crops influences the type and levels of prebiotic carbohydrates present. Pulses such as common bean and chickpea are rich sources of prebiotic carbohydrates, including sugar alcohols (SAs), raffinose family oligosaccharides (RFOs), fructooligosaccharides (FOSs), resistant starch (RS), and amylose. This study determined the changes in prebiotic carbohydrate concentrations of seven common bean and two chickpea market classes after thermal processing (cooking, cooling, and reheating). A 100-g serving of common bean provides 0.7 to 10.6 mg of SAs, 3.9 to 5.2 g of RFOs, 57 to 143 mg of FOSs, 2.6 to 3.9 g of RS, and 25 to 33 g of amylose; cooling and reheating reduced RFOs but increased SAs, FOSs, and RS in many cases. A 100-g serving of chickpea (cooked at 90 °C for 4 hr) provides 1.2 to 1.7 g of SAs, 2.5 to 3.2 g of RFOs, 26 to 43 mg of FOSs, 3.6 to 5.3 g of RS, and 24 to 30 g of amylose; cooling and reheating reduced SAs and RFOs but increased FOSs, RS, and amylose concentrations. Processing methods change the nutritional quality of pulse crops by changing the type and quantity of prebiotic carbohydrates. © 2020 Institute of Food Technologists®.INTRODUCTION Endometrial cancer (EC) is the most common gynecological malignancy in the developed world. The prognosis of EC strongly depends on tumor stage, hence the importance of improving diagnosis. Metabolomics has recently appeared as a promising test for a non-invasive diagnosis of several diseases. Nevertheless, no metabolic marker has been approved for use in the routine practice. We aimed to provide an overview of metabolomics findings in the diagnosis of EC. MATERIAL AND METHODS A systematic review was performed by searching 8 electronic databases from their inception to October 2019 for studies assessing metabolomics in EC diagnosis. Extracted data included characteristics of patients and EC, serum concentration of metabolites in women with and without EC and its association with EC diagnosis, tumor behavior and pathological characteristics. RESULTS Six studies with 732 women (356 cases and 376 controls) were included. Several metabolites were found able to predict the presence of EC, tumor behavior (progression and recurrence) and pathological characteristics (histotype, myometrial invasion, lymph vascular space invasion).
Homepage: https://www.selleckchem.com/products/Mycophenolic-acid(Mycophenolate).html
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