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cells. The excellent performance of the probe makes it applying for the visualization detection of endogenous cysteine in living cells and tissues with obtaining satisfactory results.An alternative optical signal transduction mechanism for ion-selective optodes is proposed. The nanostructural sensors benefit from ion-selective reversing aggregation caused quenching yielding turn-on, bright and highly stable optical signals. Selective incorporation of analyte results in transformation of the polymer dye from aggregate to a micelle structure, affecting spatial arrangement of chromophore groups in the nanostructure. Formation of micelles, induced by ion-selective interactions, is coupled with pronounced increase of emission due to decrease of aggregation caused quenching, characteristic for dispersed phase formation. The formed micelles are highly stable in solution, offering constant in time (days scale) emission signal. DNA chemical The important difference from other known systems is that the analyte binding induced change does not affect the chromophore group, but occurs in distant, terminal position of the side chain of the polymer. As a model system calcium selective optodes have been prepared. Thus obtained probes were characterized with broad analyte concentration range (from 10-7 to 10-3 M) emission signal increase. The turn-on response was observed within broad range of pH (6.3-8.9), with no sign of optical signal deterioration during 5 days contact with the analyte or more than two weeks storage.Dibenzothiophene (DBT) and its derivatives are important constituents of organosulfur compound in fossil fuels, which can result in one type of "acid rain" after burning process. Several technologies and methods have been developed to detect DBT, but they can be sophisticated and expensive. We have developed a two-step in-situ reduction method to fabricate Ag NPs modified glass fiber paper for SERS detection of DBT and its derivatives in a convenient and cheap way. Different from previous reports showing DBT cannot be detected by SERS, the substrate fabricated by our method revealed DBT's characteristic Raman peak at 1600 cm-1. The dense and multilayer Ag NPs on glass fiber provided abundant spatial surface for DBT absorption and chemical interaction with Ag NPs, which led to CHEM enhancement in SERS detection. The mechanism was verified by UV-visible absorption spectrum and calculated Raman spectra. There was a good linear relationship between the SERS intensity at 1600 cm-1 and the concentration of DBT solution between 1 × 10 -5 and 1 × 10-3 mol/L and the limit of detection was 1 × 10-6 mol/L. Spiked petrol sample was detected and the recovery rate of DBT is in the range of 94.53%-107.39%. This method provides a convenient and reliable way to detect DBT and its derivatives.A variety of fluorescence probes have been developed for fluorescence imaging of metals in biological cells. However, accurate quantification of metals with fluorescent approaches is challenging due to the difficulty in establishing a standard calibration curve in living cells. Herein, a fluorescence imaging protocol is developed for imaging intracellular Cu2+ and its correlation with the cellular uptake of copper. The total amount of intracellular Cu is detected by inductively coupled plasma mass spectrometry (ICP-MS) in parallel. Fluorescence imaging of Cu2+ is accomplished with Rhodamine B derivative modified carbon dots (CDs-Rbh) based on fluorescence resonance energy transfer (FRET) from CDs to rhodamine. Intracellular Cu2+ is correlated with fluorescence ratio at λem 500-600 nm (rhodamine) to λem 425-475 nm (CDs) with excitation at λex 405 nm. It is found that Cu2+ is linearly correlated with the total intracellular uptaken copper content, with a linear correlation between the relative fluorescence ratio in fluorescence imaging and intracellular Cu derived from ICP-MS, including both Cu(I) and Cu(II) species. The linear calibration equation is lg(F2/F1) = 0.00148 m[Cu]-0.3622. This approach facilitates further investigation and elucidation of copper transition in live cells and the evaluation of their cytotoxicity.Metal-organic framework materials (MOFs) are highly promising materials for biomedical applications owing to high porosity, adjustable pore structure and high loading capacity. In this paper, we herein reported a novel UiO-66-NH2 MOF-based ratiometric fluorescent probe for the high sensitive detection of dopamine and reduced glutathione. Light-emitting metal-organic framework materials UiO-66-NH2 MOF with a fluorescence emission wavelength of 450 nm was synthesized by a simple hydrothermal synthesis. Dopamine could self-oxidize in polyethyleneimine (PEI) solution to form copolymer (PDA-PEI), which can emit yellow-green fluorescence at 530 nm. PDA-PEI can quench the fluorescence of UiO-66-NH2 MOF via FRET and the fluorescence intensity of PDA-PEI at 530 nm is increasing. Due to the reductive properties of glutathione, the formation of PDA-PEI could be blocked and the fluorescence of the UiO-66-NH2 MOF could be restored. Therefore, dopamine and reduced glutathione could be detected simultaneously via monitoring the ratiometric fluorescence intensity (I530/I450). The ratiometric fluorescent method showed good linearity curve with the concentration of dopamine in the range of 4-50 μM and with the concentration of reduced glutathione in the range of 1-70 μM. Furthermore, the ratiometric fluorescent method had a low detection limit for DA (0.68 μM) and GSH (0.57 μM), and was successfully applied for DA and GSH determination in human serum.In studies on cell therapies, cells often need to be magnetically labeled and then tracked using magnetic resonance imaging (MRI) techniques. To achieve good imaging performance on infused cells, the analysis of the sorted, labeled cells before infusion is necessary. Herein, we developed a microfluidic chip to quantitatively analyze magnetically labeled cells. The chip was equipped with a magnetophoresis-based cell sorting function and an impedance-based cell counting function. Using RAW264.7 macrophages, we confirmed the two functions of the chip, obtained the number and the magnetic loading distribution of the sorted, labeled cells, and ultimately demonstrated the broad applications of the chip in rapidly selecting a proper flow rate for the buffer solution in the cell sorting process, determining the total average magnetic loading of the labeled cells for the cell labeling process, and offering a necessary reference for the processing of the sorted cells for high performance in vivo imaging. This work provides an integrated lab-on-a-chip design for quantitatively analyzing magnetically labeled cells and thus can promote MRI-based cell-tracking studies.
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