Notes

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6 ± 2.7) mg kg-1. Bespoke arsenic speciation of edible algae samples has revealed the presence of some known inorganic and simple organic As compounds such as As(III) from less then LOD to (8.97 ± 0.59) mg kg-1, As(V) from less then LOD to (5.95 ± 0.29) mg kg-1 and DMA from less then LOD to (0.766 ± 0.040) mg kg-1. Mass balance calculation carried out on the basis of tAs and bespoke As speciation results has shown that the amount of unknown As species in edible algae samples varied from 28% to 100% of extracted tAs. Identification of uAs species in edible algae samples has shown the presence of a high variety of As-sugars (12 compounds) and confirmed the presence of simple inorganic and organic As species such as As(V) and DMA along with 8 more simple organic As compounds. The results obtained in this study have confirmed that the high amounts of tAs do not correspond to the toxicity of algae based food due to the lack of the inorganic As in the tested samples.Upconversion materials have been the focus of a large body of research in analytical and clinical fields in the last two decades owing to their ability to convert light between various spectral regions and their particular photophysical features. They emit efficient and sharp ultraviolet (UV) or visible luminescence after excitation with near-infrared (NIR) light. These features overcome some of the disadvantages reported for conventional fluorescent materials and provide opportunities for high sensitivity chemo-and bio-sensing. Here, we review studies that used upconversion materials as sensors for the determination of pharmaceuticals and biomolecules in the last two decades. The articles included in this review were retrieved from the SCOPUS database using the search phrases "upconversion nanoparticles for determination of pharmaceutical compounds", and "upconversion nanoparticles for determination of biomolecules". Details of each developed upconversion nanoparticles based sensor along with their relevant analytical parameters are reported and carefully explained.Cell signalling is tightly regulated by post-translational modification of proteins. Among them, phosphorylation is one of the most interesting and important. Identifying phosphorylation sites on proteins is challenging and requires strategies for pre-separation and enrichment of the phosphorylated species. We applied four different methods for phospho-enrichment involving TiO2 and IMAC matrix to human melanoma cell lysates of starved A375 induced for 1 h with 1% FBS. Comparison of protocol efficiency was evaluated through peptide concentration, sulphur and phosphorus content and peptide analysis by LC-MS in the collected fractions. Our results underlined that each single method is not sufficient for a comprehensive phosphoproteome analysis. In fact, each methodology permits to identify only a fraction of the phosphoproteome contained in a whole cell lysate. The selection of the most efficient protocols and a combination of two phospho-enrichment methods allowed the assessment of this workflow able to pinpoint the main actors in the phospho-proteome cascade of A375 human melanoma cells treated with Vemurafenib.The wood of the Cembran pine (Pinus cembra L.), also known as Swiss pine, Arolla pine or Stone pine, has a prominent and long-lasting odor which has been reported to relieve stress in humans. However, the odorants responsible for this are unknown and it is also unclear whether the odor profile depends on the origin of the trees, namely different geographical regions and pedoclimatic conditions. Therefore, in this study human sensory analyses together with isolation and analysis of the target odorants via solvent-assisted flavor evaporation were performed. Afterwards, comparative odor extract dilution analysis (cOEDA) was applied to investigate the odorous constituents of pinewood samples from forests in Austria and Italy. MIK665 price Specific odorants were thereby identified using one-dimensional and two-dimensional gas chromatography-mass spectrometry/olfactometry using two capillary columns with different polarities (DB-5 and DB-FFAP). In total, 103 odorants were detected with successful identification of 98 of these substances. The identified odorants were predominately monoterpenes and sesquiterpenes as well as organic acids. Several compounds including germacrene D, thymol, carvacrol, rotundone, β-caryophyllene, α-humulene, cinnamaldehyde, and ethyl cinnamate are reported here for the first time as odorous constituents of pinewood. Principal component analysis of the data obtained from sensory evaluation and cOEDA indicated that terpenes and sesquiterpenes were correlated with higher hedonic rates for the wood samples. Moreover, a Mantel test between the geographical distances of the trees and their sensory and cOEDA values demonstrated that the origin of the trees had a significant impact on the sensory characteristics of the wood, in agreement with the differences in the relative proportions of different volatile components. As such, the odor of Cembran pinewood is prone to variation but, yet, unique.Bimodal photoluminescence-magnetic resonance (MR) imaging technique has attracted tremendous attention due to its great potential in biomedical researches and clinical practices. Herein a novel multifunctional magnetoluminescent nanocomposite, FA-Gd-Tb@SiO2, was found to serve as an effective probe for bimodal time-gated luminescence/MR imaging of cancer cells in vitro and in vivo. The nanoprobe was designed by integrating a luminescent Tb3+ complex, a Gd(III)-based contrast agent and a tumor-targeting molecule, folic acid (FA), into a silica nanoparticle. The integration of these functional moieties allows the nanoprobe to be employed for specific imaging of cancer cells with background-free TGL and non-invasive MR imaging modes. In addition, the optical and magnetic properties were dramatically improved after implicating the newly synthesized nanoarchitecture. In vitro cellular TGL imaging demonstrated that the FA-Gd-Tb@SiO2 nanoprobe could recognize and accumulate in cancer cells overexpressing FA receptor.
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