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The ongoing global pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a public health emergency of international concern. Although SARS-CoV-2 is considered to be mainly transmitted by inhalation of contaminated droplets and aerosols, SARS-CoV-2 is also detected in human feces and to a less extent in urine, and in raw wastewaters (to date viral RNA only) suggesting that other routes of infection may exist. see more Monitoring SARS-CoV-2 genomes in wastewaters has been proposed as a complementary approach for tracing the dynamics of virus transmission within human population connected to wastewater network. The understanding on SARS-CoV-2 transmission through wastewater surveillance, the development of epidemic modeling and the evaluation of SARS-CoV-2 transmission from contaminated wastewater are largely limited by our knowledge on viral RNA genome persistence and virus infectivity preservation in such an environment. Using an integrity based RT-qPCR assay this study led to the discovery that SARS-CoV-2 RNA can persist under several forms in wastewaters, which provides important information on the presence of SARS-CoV-2 in raw wastewaters and associated risk assessment.UV254 irradiation disinfection is a commonly used method to inactivate pathogenic viruses in water and wastewater treatment. Model prediction method can serve as a pre-screening tool to quickly estimate the effectiveness of UV254 irradiation on emerging or unculturable viruses. In this study, an improved prediction model was applied to estimate UV254 photolysis kinetics of viral genomes (kpred, genome) based on the genome sequences and their photoreactivity and to correlate with the experimental virus infectivity loss kinetics (kexp, infectivity). The UV254 inactivation data of 102 viruses (including 2 dsRNA, 65 ssRNA, 33 dsDNA and 2 ssDNA viruses) were collected from the published experimental data with kexp, infectivity ranging from 0.016 to 3.49 cm2 mJ-1. The model had fairly good performance in predicting the virus susceptibility to UV254 irradiation except dsRNA viruses (Pearson's correlation coefficient = 0.64) and 70% of kpred, genome fell in the range of 1/2 to 2 times of kexp, infectivity. The positive deviation of the model often occurred for photoresistant viruses with low kexp, infectivity less than 0.20 cm2 mJ-1 (e.g., Adenovirus, Papovaviridae and Retroviridae). We also applied this model to predict the UV254 inactivation rate of SARS-CoV-2 (kpred, genome = 3.168 cm2 mJ-1) and a UV dose of 3 mJ cm-2 seemed to be able to achieve a 2-log removal by conservative calculation using 1/2kpred, genome value. This prediction method can be used as a prescreening tool to assess the effectiveness of UV254 irradiation for emerging/unculturable viruses in water or wastewater treatment.Many lowland regions are afflicted with high phosphorus (P) peaks in rivers during the summer months. Static incubations of sediments have shown that reductive dissolution of ferric iron (Fe(III)) minerals in the sediment explain these P peaks. This study was set up to identify if that mechanism also dominates in a dynamic system, thereby testing the roles of water flow velocity and sediment Fe/P ratio. Decreasing flow velocity was suspected to lower the flux of dissolved oxygen (DO) towards the sediment. The role of the Fe(III)/P ratio was tested by amending iron-rich glauconite sand (GS) to the sediment, in this manner testing possible remediation techniques. Eight flumes (1.80 m long) were constructed with duplicates of four treatments of two laminar flow velocities over the sediment (0.05 m s-1 or 0.15 m s-1) that was either or not amended with GS (10% w/w). In all flumes a daily dose of sodium glutamate was added as a carbon source to mimic wastewater with high BOD, the flumes were operated for 28 days. A decreased velocity lowered the steady-state DO concentration and enhanced the sediment-water release of P by a factor 3. Sediment amendment with GS reduced solution P by factors 3 (low flow velocity) and 2 (high flow velocity). This effect is related to a combination of increasing binding sites for P and of lowering the DO consumption. These experimental data suggest that previously unexplained summer peaks of P in lowland rivers are related to low flow events that limit the DO flux. The internal loading of P requires management of DO in water and can be mitigated by enhancing sediment Fe.Extracellular Polymeric Substances (EPS) existent in anaerobic sludge proves to be a barrier for sludge liquefaction and biomass lysis efficiency. Hence EPS deaggregation heightens the surface area for the subsequent pretreatment thereby uplifting the sludge disintegration and biomethanation rate. This review documents the role of EPS and its components which inhibits sludge hydrolysis and also the various phase separated pretreatment methods available with its disintegration mechanism to enhance the biomass lysis and methane production rate. It also illustrates the effects of phase separated pretreatment on the sludge disintegration rate which embodies two phases-floc disruption and cell lysis accompanied by their computation through biomethane potential assay and fermentation analysis comprehensively. Additionally, energy balance study and cost analysis requisite for successful implementation of a proposed phase separated pretreatment on a pilot scale level and their challenges are also reviewed. Overall this paper documents the potency of phase separated pretreatment for full scale approach.The nitrous oxides emission was measured over 7 months in the full-scale aerobic granular sludge plant in Dinxperlo, the Netherlands. Nitrous oxide concentrations were measured in the bulk liquid and the off-gas of the Nereda® reactor. Combined with the batch wise operation of the reactor, this gave a high information density and a better insight into N2O emission in general. The average emission factor was 0.33% based on the total nitrogen concentration in the influent. The yearly average emission factor was estimated to be between 0.25% and 0.30%. The average emission factor is comparable to continuous activated sludge plants, using flocculent sludge, and it is low compared to other sequencing batch systems. The variability in the emission factor increased when the reactor temperature was below 14 °C, showing higher emission factors during the winter period. A change in the process control in the winter period reduced the variability, reducing the emission factors to a level comparable to the summer period.
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